赤水乌骨鸡肌肉生长抑制素基因多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2017.04.025

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 1115-1121.doi: 10.16431/j.cnki.1671-7236.2017.04.025

赤水乌骨鸡肌肉生长抑制素基因多态性及其与肉质性状的关联分析

李维, 祖盘玉, 李洪林, 林家栋, 张福平

贵州大学动物科学学院, 高原山地动物遗传育种与繁殖教育部重点实验室, 贵州省动物遗传育种与繁殖重点实验室, 贵阳 550025

收稿日期:2016-11-18 出版日期:2017-04-20 发布日期:2017-05-03
通讯作者: 张福平 E-mail:zfu-1010@126.com
作者简介:李维(1988-),男,湖南永州人,硕士,研究方向:特种畜禽饲养,E-mail:gzdxliwei@163.com
基金资助:
贵州省科学技术基金（黔科合J字[2012]2147号）

Polymorphisms of MSTN Gene and Its Association with Meat Quality Traits in Chishui Black-bone Chicken

LI Wei, ZU Pan-yu, LI Hong-lin, LIN Jia-dong, ZHANG Fu-ping

Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Sciences, Guizhou University, Guiyang 550025, China

Received:2016-11-18 Online:2017-04-20 Published:2017-05-03

摘要/Abstract

摘要：

本研究旨在探讨肌肉生长抑制素（myostatin，MSTN）基因多态性及其与赤水乌骨鸡肉质的关联性。采用DNA池、PCR产物直接测序方法对赤水乌骨鸡MSTN基因的外显子进行多态性检测，结果发现，在赤水乌骨鸡MSTN基因的外显子1中检测到1个SNP位点C324T，表现为3种基因型，分别命名为CC、CT和TT，而在MSTN基因外显子2、3上没有发现多态位点。对基因型与肉质性状关联分析显示，CT基因型胸肌、腿肌pH均显著高于CC和TT基因型（P<0.05），CT基因型剪切力显著高于TT基因型（P<0.05），其他肉质性状在不同基因型间差异均不显著（P>0.05）。此结果提示，MSTN基因对赤水乌骨鸡pH和剪切力具有较大的遗传效应，初步推断 MSTN是控制这一性状的众多基因之一，且可能是影响赤水乌骨鸡pH和剪切力的一个主效基因或与主效基因相连锁，可作为选育赤水乌骨鸡pH和剪切力的分子标记，用于标记辅助选择意义重大。

关键词: 赤水乌骨鸡; MSTN基因; 肉质

Abstract:

This study was aimed to analyze the association of myostatin (MSTN) gene polymorphisms with meat quality traits in Chishui Black-bone chicken. The DNA pooling was constructed and the direct sequencing technology of PCR product was used to detect the single nucleotide polymorphism (SNP) of MSTN gene. The results showed that C324T were detected in exon 1 of MSTN gene in Chishui Black-bone chicken. However, there was not found SNP site in exon 2 and exon 3 of MSTN gene. The C324T site was devised into three genotypes of CC, CT and TT. The association relationships between the genotype of MSTN gene and meat quality traits revealed that chest muscle pH and leg muscle pH value of Chishui Black-bone chicken with CT genotype were significantly higher than that of CC and TT genotypes (P<0.05), the shear force of CT genotype was significantly higher than that of TT genotype (P<0.05), there was no significant difference in other meat quality traits (P>0.05). This results suggested that the pH value and shear force of MSTN gene had a larger genetic effect in Black-bone chicken,MSTN gene was one of genes which could control the preliminary inference, might affect the pH value and shear force or with the main gene chain in Black-bone chicken, and could be used as pH value and shear force of molecular markers of Black-bone chicken.

Key words: Chishui Black-bone chicken; MSTN gene; meat quality

中图分类号:

S813.3

李维, 祖盘玉, 李洪林, 林家栋, 张福平. 赤水乌骨鸡肌肉生长抑制素基因多态性及其与肉质性状的关联分析[J]. 《中国畜牧兽医》, 2017, 44(4): 1115-1121.

LI Wei, ZU Pan-yu, LI Hong-lin, LIN Jia-dong, ZHANG Fu-ping. Polymorphisms of MSTN Gene and Its Association with Meat Quality Traits in Chishui Black-bone Chicken[J]. , 2017, 44(4): 1115-1121.

参考文献

[1] Matsakas A. Myostatin tilts the balance between skeletal muscle size,function and metabolism[J]. Experimental Physiology,2014,99(3):469-470.
[2] Wang Y,Wang X,Meng X,et al. Identification of two SNPs in myostatin (MSTN) gene of Takifugu rubripes and their association with growth traits[J]. Molecular & Cellular Probes,2014,28(4):200-203.
[3] Patel A K,Tripathi A K,Patel U A,et al. Myostatin knockdown and its effect on myogenic gene expression program in stably transfected goat myoblasts[J].In vitro Cellular & Developmental Biology-Animal,2014,50(7):587-596.
[4] Bagatoli A,Gasparino E,Soares M A,et al. Expression of calpastatin and myostatin genes associated with lamb meat quality[J].Genetics & Molecular Research Gmr,2013,12(4):6168-6175.
[5] Cao S Z,Yue C H,Li X R,et al. Production of myostatin gene knockout Wuzhishan miniature pig fibroblasts with zinc-finger nucleases[J].Yi Chuan,2013,35(6):778-785.
[6] Proserpio V,Fittipaldi R,Ryall J G,et al. The methyltransferase SMYD3 mediates the recruitment of transcriptional cofactors at the myostatin and c-Met genes and regulates skeletal muscle atrophy[J]. Genes & Development,2013,27(11):1299-1312.
[7] Gao F,Sun B,Xing S,et al. The effect of leader peptide mutations on the biological function of bovine myostatin gene[J].Gene,2014,540(2):171-177.
[8] Xu T S,Gu L H,Zhang X H,et al. Characterization of myostatin gene (MSTN) of Pekin duck and the association of its polymorphism with breast muscle traits[J]. Genetics & Molecular Research Gmr,2013,12(3):532-533.
[9] 赵南南,陈永华,刘倩,等. 高邮鸭肌肉生长抑制素基因(MSTN) 5'调控区多态性与生长及腹脂率的关联分析[J]. 畜牧与兽医,2013,45(7):9-13.
[10] Mcpherron A C,Lawler A M,Lee S J. Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member[J]. Nature,1997,387(6628):83-90.
[11] Clop A,Marcq F,Takeda H,et al. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep[J]. Nature Genetics,2006,38(7):813-818.
[12] 潘英树,张永宏,高妍,等. 朗德鹅MSTN基因外显子3多态性的研究[J]. 吉林农业科学,2008,33(6):30-31.
[13] 胡兰,王娜,胡锐,等. 大骨鸡MSTN基因的表达检测[J]. 中国家禽,2003,7(s1):46-48.
[14] 卢俊清. 北京鸭肌肉生长抑制素基因(MSTN)多态性与骨骼肌和脂肪生长发育性状的关联性分析[D]. 北京:中国农业科学院,2008.
[15] 姚力丹,祖玲玲,马晓燕,等. 新疆5个品种羊不同月龄MSTN基因mRNA表达水平及其与生长性状相关性分析[J]. 中国畜牧兽医,2016,43(2):462-470.
[16] 顾志良,朱大海,李宁,等. 鸡Myostatin基因单核苷酸多态性与骨骼肌和脂肪生长的关系[J]. 中国科学(生命科学),2003,33(3):273-280.
[17] 卢俊清,黄苇,赵楠,等. 北京鸭肌肉生长抑制素基因多态与屠体性状关系分析[J]. 中国畜牧兽医,2008,35(10):61-64.
[18] 朱智,吴登俊,徐宁迎. 鸡Myostatin基因单核苷酸多态性及其对屠体性状的遗传效应分析[J]. 遗传,2007,29(5):593-598.
[19] 顾志良,张海峰,朱大海,等. 鸡Myostatin基因单核苷酸多态性的群体遗传学分析[J]. 遗传学报,2002,29(7):599-606.
[20] Zhang G X,Zhao X H,Wang J Y,et al. Effect of an exon 1 mutation in the myostatin gene on the growth traits of the Bian chicken[J]. Anim Genet,2012,43(4):458-459.
[21] Zhang G,Ding F,Wang J,et al. Polymorphism in exons of the myostatin gene and its relationship with body weight traits in the Bian chicken[J]. Biochem Genet,2011,49(1/2):9-19.
[22] 魏伍川,刘欣,陈耀玲. 湛江三黄鸡2个脂肪性状相关基因的多态性及其与屠宰腹脂性状的相关分析[J]. 广东农业科学,2013,2:95-98.
[23] 胡师金,张淑珍,袁泽湖,等. 绵羊CLPG和MSTN基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医,2016,43(5):1285-1293.
[24] 吴鹏,黄美玲,李尤简,等. 绵羊肌肉生长抑制素(MSTN)双价纳米抗体构建与表达[J]. 黑龙江畜牧兽医,2016,17:42-45.
[25] 张冬杰,刘娣,张旭,等. 利用CRISPR-Cas9系统定点突变猪MSTN基因的研究[J]. 畜牧兽医学报,2016,47(1):207-212.

赤水乌骨鸡肌肉生长抑制素基因多态性及其与肉质性状的关联分析

Polymorphisms of MSTN Gene and Its Association with Meat Quality Traits in Chishui Black-bone Chicken

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