伊犁马MyoG基因外显子1多态性与肉质性状及肌纤维性状关联分析

doi:10.16431/j.cnki.1671-7236.2021.07.026

摘要/Abstract

摘要： 为寻找伊犁马肉质性能的分子标记，试验以38匹伊犁马为材料，测定肉质性状（失水率、熟肉率、剪切力）和肌纤维性状（肌纤维横截面积、肌纤维直径、肌纤维密度），利用PCR直接测序法检测肌细胞生成素（meyogenin，MyoG）基因外显子1在伊犁马群体中的多态性，并对MyoG基因SNPs不同基因型与肉质、肌纤维性状进行关联分析。结果表明，MyoG基因外显子1检测出5个突变位点，分别为SNP1（g.31187343 A>C）、SNP2（g.31187333 G>A）、SNP3（g.31187132 C>T）、SNP4（g.31187105 C>G）和SNP5（g.31187099 C>T），其中SNP1为错义突变，碱基A突变为C使得氨基酸由苏氨酸突变为脯氨酸，其他位点均为无义突变。SNP3和SNP4为中度多态位点，SNP1和SNP2为低度多态位点，这4个位点均处于Hardy-Weinberg平衡状态。MyoG基因外显子1中SNP1和SNP4不同基因型个体失水率、熟肉率、肌纤维横截面积、肌纤维直径、肌纤维密度差异显著（P<0.05）；SNP3不同基因型个体熟肉率、肌纤维横截面积、肌纤维密度差异显著（P<0.05）；SNP2不同基因型个体各指标差异均不显著（P>0.05）。综上，伊犁马MyoG基因外显子1检测到5个多态位点，其中SNP1（g.31187343 A>C）、SNP3（g.31187132 C>T）和SNP4（g.31187105 C>G）位点不同基因型对肉质及肌纤维性状有显著影响，这些位点可作为伊犁马肉质性能潜在分子标记。

关键词: 伊犁马; MyoG基因; 肉质性状; 肌纤维性状; 关联性

Abstract: In order to find molecular markers for meat quality of Yili horses,38 Yili horses were used to determine meat quality traits and muscle fiber traits (including water loss rate,cooked meat rate,shear force,muscle fiber cross-sectional area,muscle fiber diameter and muscle fiber density).The polymorphism of myogenin (MyoG) gene exon 1 in Yili horse population was detected by PCR direct sequencing,and the relationship between different genotypes of MyoG gene SNPs and meat quality,muscle fiber traits were analyzed.The results showed that five SNPs were detected in exon 1 of MyoG gene,including SNP1 (g.31187343 A>C),SNP2 (g.31187333 G>A),SNP3 (g.31187132 C>T),SNP4 (g.31187105 C>G) and SNP5 (g.31187099 C>T).The SNP1 was found to be a missense mutation,and the mutation of base A to C occurs,which maked the amino acid mutation from threonine to proline.The other sites were found to be nonsense mutations.SNP3 and SNP4 were found to be moderately polymorphic,while SNP1 and SNP2 were low polymorphic,the four SNPs were in Hardy-Weinberg equilibrium.Association analysis showed that:SNP1 and SNP4 genotypes in exon 1 of MyoG gene were significantly associated with water loss rate,cooked meat rate,muscle fiber cross-sectional area,muscle fiber diameter and muscle fiber density (P<0.05).SNP3 genotypes were significantly associated with cooked meat rate,muscle fiber cross-sectional area and muscle fiber density (P<0.05).There was no significant correlation between SNP2 genotypes and each index (P>0.05).In conclusion,five SNPs were detected in exon 1 of MyoG gene in Yili horse,among which SNP1 (g.31187343 A>C),SNP3 (g.31187132 C>T) and SNP4 (g.31187105 C>G) had significant effects on meat quality and muscle fiber traits.These SNPs might become molecular markers of meat quality in Yili horse.

Key words: Yili horse; MyoG gene; meat quality traits; muscle fiber traits; correlation

中图分类号:

S821.2

李云霞, 姚新奎, 孟军, 曾亚琦, 王建文, 郑国鹏, 张月, 袁鑫鑫, 王彤亮, 李云涛. 伊犁马MyoG基因外显子1多态性与肉质性状及肌纤维性状关联分析[J]. 中国畜牧兽医, 2021, 48(7): 2504-2511.

LI Yunxia, YAO Xinkui, MENG Jun, ZENG Yaqi, WANG Jianwen, ZHENG Guopeng, ZHANG Yue, YUAN Xinxin, WANG Tongliang, LI Yuntao. Correlation Analysis Between Polymorphism of MyoG Gene Exon 1 With Meat Quality Traits and Muscle Fiber Traits in Yili Horse[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2504-2511.

参考文献

[1] 叶再华,姚新奎,潘小东,等.伊犁马屠宰性能测定及适宜屠宰年龄研究[J].新疆农业大学学报,2008,3:13-15. YE Z H,YAO X K,PAN X D,et al.A study on the slaughter performance and optimal slaughter ages for Yili horse[J].Journal of Xinjiang Agricultural University,2008,3:13-15.(in Chinese)
[2] FRANCO D,LORENZO J M.Effect of muscle and intensity of finishing diet on meat quality of foals slaughtered at 15 months[J].Meat Science,2014,96(1):327-334.
[3] BOLEMAN S J,BOLEMAN S L,MILLER R K,et al.Consumer evaluation of beef of known categories of tenderness[J].Journal of Animal Science,1997,75(6):1521.
[4] 刘莉敏,郭军,木其尔,等.蒙古马肉常规营养素和脂肪酸分析评价[J].中国食物与营养,2017,23(1):64-68. LIU L M,GUO J,MU Q E,et al.Analysis and evaluation of nutrients and fatty acids of Mongolian horsemeat[J].Food and Nutrition in China,2017,23(1):64-68.(in Chinese)
[5] 周磊.不同品种畜禽肉品质参数的比较研究[D].乌鲁木齐:新疆农业大学,2007. ZHOU L.The comparison study of meat quality parameter on differents domastic livestock and poultry breeds[D].Urumqi:Xinjiang Agricultural University,2007.(in Chinese)
[6] DAVEG R D H C,BROHOLM C,PERFILYEV A,et al.Abnormal epigenetic changes during differentiation of human skeletal muscle stem cells from obese subjects[J].BMC Medicine,2017,15(1):39.
[7] LIU Z,ZHANG X,LEI H,et al.CASZ1 induces skeletal muscle and rhabdomyosarcoma differentiation through a feed-forward loop with MYOD and MYOG[J].Nature Communications,2020,11(1):911.
[8] KLEIN W H,HASTY P,BRADLEY A,et al.Muscle deficiency and neonatal death in mice with a targeted mutation in the myogenin gene[J].Nature,1993,364(6437):501.
[9] FRANCETIC T,QIAO L.Skeletal myogenesis and Myf5 activation[J].Transcription,2011,2(3):109-114.
[10] MORESI V,WILLIAMS A H,MEADOWS E,et al.Myogenin and class Ⅱ HDACs control neurogenic muscle atrophy by inducing E3 ubiquitin ligases[J].Cell,2010,143(1):35-45.
[11] HONG T A,HUEY J,JAKOB S,et al.Analysis of binding and activating functions of the chick muscle acetylcholine receptorγ-subunit upstream sequence[J].Cellular and Molecular Neurobiology,1992,12(3):241-258.
[12] ZHAO X H,LI M Y,XU S S,et al.Expression of Myostatin (MSTN) and Myogenin (MyoG) genes in Zi and Rhine goose and their correlation with carcass traits[J].Brazilian Journal of Poultry Science,2019,21(1):1-6.
[13] 中华人民共和国农业部.畜禽肉质的测定:NY/T 1333-2007[S].北京:中国农业出版社,2007. MINISTRY OF AGRICULTURE OF THE PEOPLE'S REPULIC OF CHINA.Determination of livestock and poultry meat quality:NY/T 1333-2007[S].Beijing:Chinese Agriculture Press,2007.(in Chinese)
[14] 中华人民共和国农业部.肉嫩度的测定剪切力测定法:NY/T 1180-2006[S].北京:中国农业出版社,2006. MINISTRY OF AGRICULTURE OF THE PEOPLE'S REPUBLIC OF CHINA.Determination of meat tenderness shear force method:NY/T 1180-2006[S].Beijing:Chinese Agriculture Press,2006.(in Chinese)
[15] 杨东树.伊犁地区杂交肉用马的体重体尺和屠宰性状及肌肉组织学的研究[D].乌鲁木齐:新疆农业大学,2016. YANG D S.Measurement and analysis of hybrid meat horse on body weight,measurements and muscle histology in Yili area[D].Urumqi:Xinjiang University Agricultural,2016.(in Chinese)
[16] WRIGHT W E,SASSOON D A,LIN V K.Myogenin,a factor regulating myogenesis,has a domain homologous to MyoD[J].Cell,1989,56(4):607-617.
[17] 孙奴奴,刘瑾.猪MyoG基因外显子1的多态性分析[J].运城学院学报,2020,38(3):5-8. SUN N N,LIU J.Polymorphism analysis of exon 1 of porcine MyoG gene[J].Journal of Yuncheng University,2020,38(3):5-8.(in Chinese)
[18] 储明星,何远清,王金玉,等.绵羊肌细胞生成素基因外显子1的PCR-SSCP分析[J].农业生物技术学报,2005,13(1):77-80. CHU M X,HE Y Q,WANG J Y,et al.PCR-SSCP analysis on exon 1 of Myogenin gene in sheep.[J].Journal of Agricultural Biotechnology,2005,13(1):77-80.(in Chinese)
[19] 易恒洁,李辉,杨胜林,等.三穗鸭MyoG基因多态性与屠宰性状的相关性研究[J].中国畜牧杂志,2016,52(9):5-10. YI H J,LI H,YANG S L.et al.Polymorphisms of MyoG gene and its relationship with slaughter traits in Sansui duck[J].Chinese Journal of Animal Science,2016,52(9):5-10.(in Chinese)
[20] 彭邦星,李辉,易恒洁,等.兴义鸭MyoG基因SNPs检测及生物信息学分析[J].黑龙江畜牧兽医,2016,15:72-76. PENG B X,LI H,YI H J,et al.SNPs detection and bioinformatics analysis of MyoG genes in Xingyi ducks[J].Heilongjiang Animal Science and Veterinary Medicine 2016,15:72-76.(in Chinese)
[21] 刘宏祥,徐文娟,宋卫涛,等.高邮鸭群体MSTN、MyoD1和MyoG基因外显子中SNP位点的分析[J].江苏农业学报,2015,31(3):604-612. LIU H X,XU W J,SONG W T,et al.Analysis of single nucleotide polymorphisms(SNPs) in exons of MSTN,MyoD1 and MyoG genes of Gaoyou duck[J].Jiangsu Journal of Agricultural Sciences,2015,31(3):604-612.(in Chinese)
[22] 朱超杰,张立春,秦立红,等.不同品种牛MyoG基因exon 1多态性比较分析[J].中国兽医学报,2016,36(6):1024-1027. ZHU C J,ZHANG L C,QIN L H,et al.Polymorphism analysis on exon 1 of MyoG gene in different cattle population[J].Chinese Journal of Veterinary Science,2016,36(6):1024-1027.(in Chinese)
[23] HESTAND M S,KALBFLEISCH T S,COLEMAN S J,et al.Annotation of the protein coding regions of the equine genome[J].PLoS One,2015,10(6):e0124375.
[24] 张增荣,朱庆,蒋小松,等.钙蛋白酶Ⅰ(CAPN1)基因多态性与鸡肉嫩度和屠体性状的相关研究[J].遗传,2007,29(8):982-988. ZHANG Z R,ZHU Q,JIANG X S.et al.Study on correlation between single nucleotide polymorphism of CAPN1 gene and muscle tenderness and carcass traits in chicken[J].Hereditas,2007,29(8):982-988.(in Chinese)
[25] 白俊艳,杨又兵,王玉琴,等.绵羊MyoG基因外显子1的多态性及其与肉质性状的关联分析[J].中国畜牧兽医,2017,44(6):1778-1783. BAI J Y,YANG Y B,WANG Y Q,et al.Polymorphism of MyoG gene exon 1 and its association analysis with meat quality traits in sheep[J].China Animal Husbandry & Veterinary Medicine,2017,44(6):1778-1783.(in Chinese)
[26] BAI J Y,CAO H,YANG Y B,et al.Analysis on correlation between polymorphism of MyoG gene exon I and body size traits of sheep[J].Indian Journal of Animal Research,2020,54(2):138-142.
[27] 李丰耘,张茹,王坤,等.盐津乌骨鸡MyoG和IGF-1基因表达及其与生长、屠宰性状的相关性分析[J].中国畜牧兽医,2020,47(8):2502-2509. LI F Y,ZHANG R,WANG K,et al.Expression of MyoG and IGF-1 Genes and its correlation analysis with growth and slaughter traits of Yanjin Black-bone chickens[J].China Animal Husbandry & Veterinary Medicine,2020,47(8):2502-2509.(in Chinese)
[28] 牛瑞娟.氨基酸突变对四种蛋白质与其配体相互作用影响的分子动力学模拟研究[D].长春:吉林大学,2019. NIU R J.The Effect of mutations on the interactions of four proteins with ligands studied by molecular dynamics simulations[D].Changchun:Jilin University,2019.(in Chinese)
[29] 岳武成,杨鹤,侯鑫,等.中华绒螯蟹MSTN基因同义突变对基因转录、翻译效率的影响[J].水产学报,2021,45(4):497-504. YUE W C.YANG H.HOU X.et al.Effects of synonymous mutation on transcription and translation efficiency of Es-MSTN gene in Chinese mitten crab (Eriocheir sinensis)[J].Journal of Fisheries of China,2021,45(4):497-504.(in Chinese)
[30] 高勤学,刘梅,杨月琴,等.猪MyoG基因的PCR-RFLP分型及其与生长性能和肌纤维数目的相关性分析[J].中国兽医学报,2005,25(3):330-332. GAO Q X,LIU M,YANG Y Q,et al.Polymorphism analysis of porcine MyoG gene by PCR-RFLP and its association with growth traits and fiber number[J].Chinese Journal of Veterinary Science,2005,25(3):330-332.(in Chinese)
[31] 郭云雁,程笃学,张龙超,等.MyoG和c-fos基因多态性及其合并基因型与猪肌纤维性状的关联分析[J].畜牧兽医学报,2013,44(2):188-196. GUO Y Y,CHENG D X,ZHANG L C,et al.Effects of single and combined genotypes of MyoG and c-fos genes on muscle fiber traits in pig[J].Chinese Journal of Animal and Veterinary Sciences,2013,44(2):188-196.(in Chinese)
[32] HUANG Y,LAI X,HU L,et al.Over-expression of DEC1 inhibits myogenic differentiation by modulating MyoG activity in bovine satellite cell[J].Journal of Cellular Physiology,2018,233(12):9365-9374.