运输对伊犁马肌肉损伤和氧化应激的影响

doi:10.16431/j.cnki.1671-7236.2021.06.012

摘要/Abstract

摘要： 试验旨在研究运输对伊犁马肌肉损伤和氧化应激的影响。选取体重相近的5匹24月龄的伊犁母马，环境适应7 d后开展运输试验，其运输过程中禁食禁水，运输总路程约为400 km，运输时间为8 h。分别于运输0（T1）、4（T2）、8（T3）和卸车后12 h （T4）采集马颈静脉血，分离血清，进行相关生理生化指标的测定。结果显示，与T1相比，肌肉损伤生化标志物肌红蛋白（MYO）和肌钙蛋白（cTnT）在T2时刻显著上升（P<0.05），T3和T4时刻极显著上升（P<0.01）；乳酸脱氢酶（LDH）在T3时刻显著上升（P<0.05）；炎症反应生化标志物C反应蛋白（CRP）和白细胞介素-6（IL-6）在T2、T3和T4时刻极显著上升（P<0.01）；皮质醇（COR）在T2和T3时刻极显著上升（P<0.01），在T4时刻显著上升（P<0.05）；总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）在T2、T3和T4时刻极显著下降（P<0.01）；过氧化氢酶（CAT）在T3和T4时刻显著下降（P<0.05）；活性氧（ROS）在T2和T4时刻显著上升（P<0.05），在T3时刻极显著上升（P<0.01）；丙二醛（MDA）在T3和T4时刻极显著上升（P<0.01）。因此，运输8 h导致了伊犁马肌肉损伤和氧化应激反应的发生，其不良影响在卸车后12 h内不能消除。

关键词: 运输; 伊犁马; 肌肉损伤; 炎症; 氧化应激

Abstract: The purpose of this study was to investigate the effects of transportation on muscle injury and oxidative stress in Ili horses.Five 24-month old Ili mares with similar body weight were selected.The transportation test was carried out after 7 days of environmental adaptation,no food or water was allowed during transportation.The total transportation distance was about 400 km and the transportation time was 8 hours.The blood samples were collected from horse jugular vein at 0 (T1),4 (T2),8 (T3) of transportation and 12 h (T4) after unloading.The serum samples were determined for related physiological and chemical indicators.The results showed that compared with T1,the muscle damage biochemical markers myoglobin (MYO) and cardiac troponin T (cTnT) increased significantly at T2(P<0.05),and increased extremely sinificantly at T3 and T4 (P<0.01).Lactic dehydrogenase(LDH) increased significantly at T3(P<0.05);The biochemical markers of inflammation C-reactive protein (CRP),interleukin-6 (IL-6) increased extremely significantly at T2,T3 and T4 (P<0.01).Cortisol(COR) increased extremely significantly at T2 and T3 (P<0.01),and increased significantly at T4 (P<0.05).Total antioxidant capacity (T-AOC),glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) decreased extremely significantly at T2,T3 and T4 (P<0.01).Catalase (CAT) decreased significantly at T3 and T4 (P<0.05).Reactive oxygen species (ROS) increased significantly at T2 and T4 (P<0.05),and increased extremely significantly at T3 (P<0.01).Malondialdehyde (MDA) increased significantly at T3 and T4 (P<0.01).Therefore,transportation could cause muscle damage and oxidative stress in Ili horses,and the adverse effects could not be eliminated within 12 h.

Key words: transportation; Ili horse; muscle damage; inflammation; oxidative stress

中图分类号:

S852

柳荦, 胡燕, 王子豪, 卢亚宾, 孙亚伟, 王金泉. 运输对伊犁马肌肉损伤和氧化应激的影响[J]. 中国畜牧兽医, 2021, 48(6): 1995-2001.

LIU Luo, HU Yan, WANG Zihao, LU Yabin, SUN Yawei, WANG Jinquan. Effects of Transportation on Muscle Damage and Oxidative Stress of Ili Horses[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1995-2001.

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