延边黄牛SCD1基因多态性及其与经济性状的关联分析

doi:10.16431/j.cnki.1671-7236.2019.09.018

摘要/Abstract

摘要： 本研究旨在探索延边黄牛硬脂酰辅酶A去饱和酶1（stearoy-CoA desaturase 1，SCD1）基因多态性及其与经济性状的相关性。以157头36月龄健康无病、体重相近的延边黄牛为研究对象，颈静脉采血提取基因组DNA，根据GenBank中牛SCD1基因序列（登录号：AC_000181.1），利用Oligo 6.0软件设计特异性引物，应用PCR扩增SCD1全长基因后直接测序，分析其多态位点，并应用生物信息学软件分析其蛋白结构；屠宰前测定活体重、胴体重、屠宰率、背膘厚等胴体性状，屠宰后采集背最长肌，测定眼肌面积、蒸煮损失、滴水损失、嫩度、系水力、pH、脂肪酸含量等肉质性状，并通过一般线性模型分析方法对延边黄牛SCD1基因SNPs与肉质性状的相关性进行了分析。结果发现，延边黄牛SCD1基因846 bp处存在A→G突变（g846 A→G）、1 022 bp处存在C→T突变（g1022 C→T），其中g1022 C→T突变导致丙氨酸（A）突变为缬氨酸（V），但未引起三级结构改变。关联分析结果表明，g846 A→G多态位点与延边黄牛宰前活重、胴体重、滴水损失及硬脂酸、亚油酸含量存在显著相关（P<0.05）；g1022 C→T多态位点与延边黄牛宰前活重、胴体重及硬脂酸含量存在显著相关（P<0.05），说明上述2个位点与延边黄牛相关肉质性状存在显著相关，该突变位点可作为潜在的分子标记。

关键词: 延边黄牛; SCD1基因; 单核苷酸多态性(SNP); 胴体性状; 肉质性状

Abstract: This study was aimed to explore the association between the polymorphism of stearoy-CoA desaturase 1 (SCD1) gene and economic traits in Yanbian Yellow cattle.157 healthy 36-month-old Yanbian Yellow cattle of similar weight were selected,and the blood genomic DNA were extracted from the jugular vein.According to the sequence of bovine SCD1 gene in GenBank (accession No.:AC_000181.1),specific primers were designed by Oligo 6.0 software.The full-length SCD1 gene was amplified by PCR and sequenced directly,the polymorphic was analyzed,and the protein structure was analyzed by bioinformatics software.The carcass traits such as live weight,carcass weight,slaughter rate and backfat thickness were measured before slaughter,the longissimus dorsi muscle was collected and the meat traits such as eye muscle area,cooking loss,drip loss,tenderness,hydraulic power,pH and fatty acid content were measured after slaughter.The correlation between the polymorphism of SCD1 gene and meat quality traits in Yanbian Yellow cattle was analyzed by general linear model analysis.The results showed that there were A→G mutation at 846 bp (g846 A→G) and C→T mutation at 1 022 bp of SCD1 gene (g1022 C→T).g1022 C→T mutation resulted in the mutation of alanie (A) to valine (V),but did not cause tertiary structural changes.Correlation analysis results showed that there was a significant correlation between g846 A→G site and the live weight,carcass weight,drip loss,and the content of stearic acid and linoleic acid (P<0.05),and there was a significant correlation between g1022 C→T site and the live weight,carcass weight and stearic acid content (P<0.05).The results indicated that the two polymorphic sites were significantly correlated with the meat quality traits of Yanbian Yellow cattle,which could be used as a potential molecular marker.

Key words: Yanbian Yellow cattle; SCD1 gene; SNPs; carcass traits; meat quality traits

中图分类号:

S813.3

朴政玉, 夏广军, 严昌国, 冯健. 延边黄牛SCD1基因多态性及其与经济性状的关联分析[J]. 中国畜牧兽医, 2019, 46(9): 2642-2650.

PIAO Zhengyu, XIA Guangjun, YAN Changguo, FENG Jian. Association Analysis Between the Polymorphism of SCD1 Gene and Economic Traits in Yanbian Yellow Cattles[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2642-2650.

参考文献

[1] HUANG G M,JIANG Q H,CAI C,et al.SCD1 negatively regulates autophagy-induced cell death in human hepatocellular carcinoma through inactivation of the AMPK signaling pathway[J]. Cancer Letters,2015,358(2):180-190.
[2] RALSTON J C,METHEREL A H,STARK K D,et al.SCD1 mediates the influence of exogenous saturated and monounsaturated fatty acids in adipocytes:Effects on cellular stress,inflammatory markers and fatty acid elongation[J].Journal of Nutritional Biochemistry,2016,27(1):241-248.
[3] MAN W C,MIYAZAKI M,CHU K,et al.Membrane topology of mouse stearoyl-CoA desaturase 1[J].Journal of Biological Chemistry,2006,281(2):1251-1260
[4] WANG J,YU L,SCHMIDT R E,et al.Characterization of HSCD5,a novel human stearoyl-CoA desaturase unique to primates[J].Biochemical and Biophysical Research Communications,2005,332(3):735-742.
[5] LENGI A J,CORL B A.Identification and characterization of a novel bovine stearoyl-CoA desaturase isoform with homology to human SCD5[J].Lipids,2007,42(6):499-508.
[6] JUN R.Isolation and molecular characterization of the stearoyl-CoA desaturase (SCD) gene affecting fat deposition in pigs[D].Germany:Degree of Doctor of Agricultural Sciences of the Faculty of Agricultuial Sciences Georg-Augst-University Gttingen,2003.
[7] CAMPBELL E M G,GALLAGHER D S,DAVIS S K,et al.Rapid communication:Mapping of the bovine stearoyl-CoA desaturase(SCD) gene to BTA26[J].American Society of Animal Science,2001,79(7):1945-1955.
[8] 卢建雄,刘翊中,臧荣鑫,等.大鼠脂肪细胞分化过程中脂代谢相关基因转录表达时序的研究[J].中国兽医科学,2006,36(4):306-310. LU J X,LIU Y Z,ZANG R X,et al.Studies on expression orders of lipid metabolism related genes during differentiation of rat adipocytes[J].Veterinary Science in China,2006,36(4):306-310.(in Chinese)
[9] VONROEMELING C A,MARLOW L A,WEI J J,et al.Stearoyl-CoA desaturase 1 is a novel molecular therapeutic target for clear cell renal cell carcinoma[J].Clinical Cancer Research,2013,19(9):2368-2380.
[10] 张静静,宋玉芹,李桢,等.中国西门塔尔牛SCD1基因多态性与肉质性状的相关分析[J].华北农学报,2013,28(1):54-57. ZHANG J J,SONG Y Q,LI Z,et al.Polymorphisms of SCD1 gene and its association with meat quality traits in Chinese Simmental[J].Acta Agriculturae Boreali-Sinica,2013,28(1):54-57(in Chinese)
[11] 郑云,刘龙,孙晓先,等.朗德鹅填肥期SCD1基因的表达及其相关miRNA的筛选[J].中国家禽,2017,39(2):16-21. ZHENG Y,LIU L,SUN X X,et al.miRNAs targeting against SCD1 gene and its expression in fatty liver formation in Landaise goose[J].China Poultry,2017,39(2):16-21.(in Chinese)
[12] MOIOLI B,CONTARINI G,AVALLI A,et al.Effect of stearoyl-coenzyme A desaturase polymorphism on fatty acid composition of milk[J].Journal of Dairy Science,2007,90(7):3553-3558.
[13] 魏聪.天府肉羊SCD1基因在肌肉中的表达与棕榈油酸和油酸含量的相关性研究[D].雅安:四川农业大学,2016. WEI C.Expression of the SCD1 gene and its relationship with the palmitoleic acid and oliec acid composition of Tianfu goat[D].Ya'an:Sichuan Agricultural University,2016.(in Chinese)
[14] 唐慧,王巍,方东辉,等.不同品种牛SCD1基因表达与肌肉脂肪酸组成的关系研究[J].黑龙江畜牧兽医,2018,1:1-4. TANG H,WANG W,FANG D H,et al.Study on the relationship between SCD1 gene expression level and muscle fatty acid composition in different breeds of cattle[J].Heilongjiang Animal Science and Veterinary Medicine,2018,1:1-4.(in Chinese)
[15] 周恒,曹艳红,朱江江,等.隆林山羊SCD基因的克隆及序列分析[J].黑龙江畜牧兽医,2016,1:80-83. ZHOU H,CAO Y H,ZHU J J,et al.Cloning and sequence analysis of SCD gene in Longlin goat[J].Heilongjiang Animal Science and Veterinary Medicine,2016,1:80-83.(in Chinese)
[16] 曹威荣.猪硬脂酰辅酶A去饱和酶蛋白生物信息学分析[J].畜牧与饲料科学,2014,35(11):5-7. CAO W R.Bioinformatics analysis of the SCD protein in pig[J].Animal Husbandry and Feed Science,2014,35(11):5-7.(in Chinese)
[17] 刘丽霞,张丽,赵生国,等.八眉猪DQA基因编码区多态性及生物信息学分析[J].华北农学报,2015,30(1):103-108. LIU L X,ZHANG L,ZHAO S G,et al.Cloning and bioinformatics analysis on coding regions of DQA gene in Bamei pigs[J]. Acta Agriculturae Boreali-Sinica,2015,30(1):103-108.(in Chinese)
[18] 夏灵敏,马晟杰,吴常文,等.曼氏无针乌贼SCD蛋白结构和功能预测[J].浙江海洋学院学报,2014,2:161-164. XIA L M,MA S J,WU C W,et al.Bioinformatic analysis and functional prognose of Sepiella maindroni de Rochebrune (SCD)[J].Journal of Zhejiang Ocean University (Natural Science),2014,33(2):161-164.(in Chinese)
[19] 周秀敏,李强子,毕英杰,等.牛SCD1基因的生物信息学分析[J].现代畜牧兽医,2016,12:30-34. ZHOU X M,LI Q Z,BI Y J,et al.Bioinformatics analysis of cattle SCD1 gene[J].Morden Journal of Animal Husbandry and Veterinary Medicine,2016,12:30-34.(in Chinese)
[20] 薛丹.G蛋白偶联受体功能位点与功能性SNPs的预测[D].上海:上海大学,2008. XUE D.Prediction of functional sites and SNPs in G protein-coupled receptors[D].Shanghai:Shanghai University,2008.(in Chinese)
[21] MASAAKI T,TAKESHI U,KENJI O,et al.Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle[J].Mammalian Genome,2004,15(2):142-148.
[22] 陶璇,张健,魏学良.牛SCD基因研究进展[J].中国草食动物,2009,4:57-59. TAO X,ZHANG J,WEI X L,et al.Advances in bovine stearoyl-coenzyme a desaturase (SCD) gene[J].China Herbivores,2009,4:57-59.(in Chinese)
[23] BARTON L,KOTT T,BURES D,et al.The polymorphisms of stearoyl-CoA desaturase(SCD1) and sterol regulatory element binding protein-1(SREBP-1) gene and their association with the fatty acid profile of muscle and subcutaneous fat in Fleckvieh bulls[J].Meat Science,2010,85(1):15-20.
[24] ZHANG S.Association of genetic variation to healthfulness of beef[A].Iowa State University Animal Industry Report[C].2005.
[25] ORRU L,CIFUNI G F,PLASENTIER E,et al.Association analyses of single nucleotide polymorphisms in the LEP and SCD1 genes on the fatty acid profile of muscle fat in Simmental bulls[J].Meat Science,2011,87(4):344-348.
[26] 刘自增,阎萍.天祝白牦牛Leptin和SCD1基因单核苷酸多态性与肌肉脂肪酸含量的关联性分析[J].中国畜牧兽医,2011,38(4):154-159. LIU Z Z,YAN P.Association analyses of SNPs in the Lpetin and SCD1 gene on the fatty acid content of intramuscular fat in Tianzhu White yak[J]. China Animal Husbandry & Veterinary Medicine,2011,38(4):154-159.(in Chinese)
[27] 施雪奎.肉牛CAPN1、CAST、GHR、SCD1基因多态性及与胴体、肉质性状的相关性研究[D].扬州:扬州大学,2011. SHI X C.Polymorphisms of CAPN1,CAST,GHR and SCD1 genes in beef cattle and their correlations with carcass and meat quality traits[D].Yangzhou:Yangzhou University,2011.(in Chinese)
[28] JIANG Z,MICHAL J J,TOBEY D J,et al.Significant associations of stearoyl-CoA desaturase (SCD1) gene with fat deposition and composition in skeletal muscle[J].International Journal of Biological Sciences,2008,4(6):345-351.
[29] 陈龙.肉牛A-FABP和SCD1基因多态性及其与肉质性状的相关性研究[D].兰州:甘肃农业大学,2014. CHEN L.Polymorphisms of A-FABP and SCD1 genes in Longdong beef cattle and its relationship with meat quality trait[D].Lanzhou:Gansu Agricultural University,2014.(in Chinese)