江口萝卜猪STAT3基因第16外显子多态性及其与肉质性状、基因表达量的相关性分析

doi:10.16431/j.cnki.1671-7236.2020.01.013

摘要/Abstract

摘要： 为研究江口萝卜猪信号传导及转录激活因子3（signal transducers and activators of transcription 3，STAT3）基因与肉质性状之间的关系，本试验测定了105头江口萝卜猪11个主要肉质性状指标，构建混合DNA池测序筛选SNP位点，单个样本直接测序验证和基因分型，并检测了不同基因型个体背最长肌中STAT3基因的表达量，利用生物统计软件分析了SNP位点与肉质性状和基因表达量之间的相关性。结果显示，在STAT3基因第16外显子上检测出1个SNP位点（Exon16-C+78T），单个样本测序只发现了CT和TT两种基因型，群体遗传参数分析发现T为优势等位基因，多态信息含量表现为中度多态，χ²检验发现该位点显著偏离了Hardy-Weinberg平衡。相关性分析发现，江口萝卜猪TT基因型大理石纹评分和熟肉率均显著高于CT基因型（P<0.05），且TT基因型剪切力显著低于CT基因型（P<0.05）；TT基因型江口萝卜猪背最长肌中STAT3基因表达量显著高于CT基因型（P<0.05）。在一定范围内，江口萝卜猪STAT3基因表达量的提高可能对于肉质性状的改善具有积极作用，TT基因型个体相较于CT基因型个体STAT3基因的表达量更高，肉质性状表现也更好。研究结果证明，STAT3基因可以作为辅助江口萝卜猪选育的候选基因，Exon16-C+78T可能是影响猪肉质性状的重要SNP位点。

关键词: 江口萝卜猪; STAT3基因; SNP; 肉质性状; 表达量; 相关性分析

Abstract: In order to study the relationship between signal transducers and activators of transcription 3 (STAT3) gene and meat quality traits in Jiangkou Luobo pigs,11 main meat quality indexes were determined in 105 Jiangkou Luobo pigs,and SNP locus were screened using mixed DNA pool sequencing.Direct sequencing and genotyping of individual samples were carried,the expression of STAT3 gene in longissimus dorsi muscle of individuals with different genotypes was detected.The correlation between SNP and meat quality and gene expression were analyzed by biostatistical software.The results showed that 1 SNP locus (Exon16-C+78T) was detected in exon 16 of STAT3 gene.Only CT and TT genotypes were found by single sample sequencing.Population genetic parameters analysis result showed that T was the dominant allele,and the polymorphism information content showed moderate polymorphism.χ² test showed that Exon16-C+78T significantly deviated from Hardy-Weinberg equilibrium.The correlation analysis between genotypes and meat quality traits showed that the marbling score and cooked meat percentage of Jiangkou Luobo pigs with TT genotype were significantly higher than that of CT genotype (P<0.05),and the shearing force of TT genotype was significantly lower than that of CT genotype (P<0.05).The expression of STAT3 gene in longissimus dorsi muscle of Jiangkou Luobo pigs with TT genotype was significantly higher than that of CT genotype (P<0.05).The increase of STAT3 gene expression in Jiangkou Luobo pigs might have a positive effect on improving meat quality,TT genotype of STAT3 gene had higher expression level than CT genotype,and the meat quality traits were better.The results indicated that STAT3 gene could be used as a candidate gene for breeding of Jiangkou Luobo pigs,and Exon16-C+78T might be an important SNP locus affecting pork quality traits.

Key words: Jiangkou Luobo pigs; STAT3 gene; SNP; meat quality; gene expression; correlation analysis

中图分类号:

S813.3

张继, 吴雪, 邱淦远, 刘鹏程, 杨茂林, 刘若余. 江口萝卜猪STAT3基因第16外显子多态性及其与肉质性状、基因表达量的相关性分析[J]. 中国畜牧兽医, 2020, 47(1): 107-115.

ZHANG Ji, WU Xue, QIU Ganyuan, LIU Pengcheng, YANG Maolin, LIU Ruoyu. Polymorphism of STAT3 Gene Exon 16 and Its Association Analysis with Meat Quality and Gene Expression in Jiangkou Luobo Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 107-115.

参考文献

[1] DARNELL J,KERR I,STARK G.Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins[J].Science,1994,264(5164):1415-1421.
[2] LIONQUE C,WARD A C.Evolution of the JAK-STAT pathway[J].JAK-STAT,2013,2(1):e22756.
[3] RICHARD A J,STEPHENS J M.Emerging roles of JAK-STAT signaling pathways in adipocytes[J].Trends in Endocrinology & Metabolism,2011,22(8):325.
[4] 吴淑芳,潘洁,孟晓芳,等.STAT3信号通路在胚胎神经系统发育中作用的研究进展[J].解剖学杂志,2018,41(4):99-101. WU S F,PAN J,MENG X F,et al.Research progress on the role of STAT3 signaling pathway in the development of embryonic nervous system[J].Chinese Journal of Anatomy,2018,41(4):99-101.(in Chinese)
[5] 牛艳珍,陈显久,王军.STAT3通路与肿瘤关系的研究进展[J].医学研究杂志,2018,3:9-11. NIU Y Z,CHEN X J,WANG J,et al.Advances in the study of the relationship between STAT3 pathway and tumor[J].Journal of Medical Research,2018,3:9-11.(in Chinese)
[6] KHATIB H,HUANG W,MIKHEIL D,et al.Effects of signal transducer and activator of transcription (STAT) genes STAT1 and STAT3 genotypic combinations on fertilization and embryonic survival rates in Holstein cattle[J].Journal of Dairy Science,2009,92(12):6186-6191.
[7] WANG Y H,HUANG M L.Organogenesis and tumorigenesis:Insight from the JAK/STAT pathway in the Drosophila eye[J].Developmental Dynamics,2010,239(10):2522.
[8] SHUAI K,STARK G,KERR I,et al.A single phosphotyrosine residue of STAT91 required for gene activation by interferon-gamma[J].Science,1993,261(5129):1744-1746.
[9] RIKU F,MELEN K,LEENA K,et al.Arginine/lysine-rich nuclear localization signals mediate interactions between dimeric STATs and importin alpha 5[J].Journal of Biological Chemistry,2002,277(33):30072-30078.
[10] KAWASHIMA T,BAO Y C,MINOSHIMA Y,et al.A rac GTPase-activating protein,MgcRacGAP,is a nuclear localizing signal-containing nuclear chaperone in the activation of STAT transcription factors[J].Molecular and Cellular Biology,2009,29(7):1796-1813.
[11] BUETTNER R,MORA L B,JOVE R.Activated STAT signaling in human tumors provides novel molecular targets for therapeutic intervention[J].Clinical Cancer Research,2002,8(4):945-954.
[12] HERRINGTON J,SMIT L S,SCHWARTZ J,et al.The role of STAT proteins in growth hormone signaling[J].Oncogene,2000,19(21):2585-2597.
[13] WANG D,ZHOU Y,LEI W,et al.Signal transducer and activator of transcription 3(STAT3) regulates adipocyte differentiation via peroxisome-proliferator-activated receptor γ (PPARγ)[J].Biology of the Cell,2003,102(1):1-12.
[14] ZHANG K,GUO W,YANG Y,et al.JAK2/STAT3 pathway is involved in the early stage the of adipognesis through regulating C/EBPP transcriptio[J].Journal of Cellular Biochemistry,2011,112(2):488-497.
[15] LANNING N J,CARTER S C.Recent advances in growth hormone signaling[J].Reviews in Endocrine and Metabolic Disorders,2006,7(4):225-235.
[16] XU D,YIN C,WANG S,et al.JAK-STAT in lipid metabolism of adipocytes[J].JAK-STAT,2013,2(4):e27203.
[17] 陈栋.IL-6通过激活JAK-STAT3通路诱导心外膜脂肪组织白色化对冠状动脉粥样硬化病变的影响[D].南京:南京大学,2015. CHEN D.The influence of whiting epicardial brown adipose tissue on coronary atherosclerosis induced by activation of JAK-STAT3 pathway[D].Guangzhou:Nanjing University,2015.(in Chinese)
[18] 杨永强,焦仁刚,龚俞,等.务川黑牛STAT3基因启动子区SNPs与生长相关性状的关联分析[J].畜牧兽医学报,2013,44(8):1205-1212. YANG Y Q,JIAO R G,GONG Y,et al.Associations of polymorphisms of promoter region of STAT3 gene with growth traits in Wuchuan Black cattle[J].Acta Veterinaria et Zootechnica Sinica,2013,44(8):1205-1212.(in Chinese)
[19] 吴贤锋.山羊STAT3和STAT5A基因SNPs、mRNA表达及甲基化对生产性能的影响[D].杨凌:西北农林科技大学,2015. WU X F.SNPs,mRNA expression and methylation of STAT3 and STAT5A genes and their influences on production traits in goats[D].Yangling:Northwest A & F University,2015.(in Chinese)
[20] 龚建军.德国猪肉品质测定技术[J].中国畜牧兽医,2005,32(9):23-26. GONG J J.German pork quality testing technology[J].China Animal Husbandry & Veterinary Medicine,2005,32(9):23-26.(in Chinese)
[21] 刘园峰,王桂芝,李秋梅,等.崂山奶山羊LEP和STAT5A基因对泌乳和生长性状的影响[J].中国农业科学,2013,46(18):3946-3954. LIU Y F,WANG G Z,LI Q M,et al.the effect of LEP and STAT5A genes on milk and growth traits of Laoshan dairy goat[J].Scientia Agriculture Sinica,2013,46(18):3946-3954.(in Chinese)
[22] 谢海强,孙岩岩,龚俞,等.贵州黑山羊STAT5A基因多态性及其与生长性状关联分析[J].生物技术,2014,4:79-83. XIE H Q,SUN Y Y,GONG Y,et al.association analysis between polymorphism of STAT5A gene and growth traits in Guizhou black goat[J].Biotechnology,2014,4:79-83.(in Chinese)
[23] 谢海强,龚俞,焦仁刚,等.贵州本地山羊STAT5A基因多态性及其与生长性状关联分析[J].基因组学与应用生物学,2014,33(4):775-781. XIE H Q,GONG Y,JIAO R G,et al.association analysis between polymorphism of STAT5A gene and growth traits in Guizhou native goat[J].Genomics and Applied Biology,2014,33(4):775-781.(in Chinese)
[24] 翟梦星.荷斯坦奶牛BRCA2基因、STAT3基因SNPs检测及其与乳房炎和产奶性状的关联分析[D].杨凌:西北农林科技大学,2015. ZHAI M X.SNPs detection of BRCA2 gene and STAT3 gene and association with mastitis and milk performances in Holstein[D].Yanglin:Northwest A & F University,2015.(in Chinese)
[25] SAUNA Z E,KIMCHISARFATY C.Understanding the contribution of synonymous mutations to human disease[J].Nature Reviews Genetics,2011,12(10):683-691.
[26] BREST P,LAPAQUETTE P,SOUIDI M,et al.A synonymous variant in IRGM alters a binding site for miR-196 and causes deregulation of IRGM-dependent xenophagy in Crohn’s disease[J].Autophagy,2011,43(7):242-245.