晋汾白猪ApoA5基因单倍型与体重和肉质性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2020.11.018

摘要/Abstract

摘要： 试验旨在探究晋汾白猪载脂蛋白A5（apolipoprotein A5，ApoA5）基因的单倍型与体重和肉质性状的相关性。选取180头晋汾白猪为研究群体，利用PCR技术检测ApoA5基因的单核苷酸多态性（single nucleotide polymorphism，SNP），并采用Haploview软件分析这些SNP位点的连锁不平衡程度；筛选非完全连锁的SNP位点，构建不同的ApoA5基因单倍型，统计不同单倍型的基因频率，并通过SPSS软件分析不同ApoA5基因单倍型与晋汾白猪体重和肉质性状的相关性，鉴定优良性状的ApoA5基因单倍型。结果显示，晋汾白猪ApoA5基因共检测到21个SNPs位点，其中5个完全连锁单倍型块，分别包含5、2、3、4和2个SNPs位点；另外10个非完全连锁的标签SNP组合成不同的ApoA5基因单倍型，其中Hap 1的基因频率最高，达到46.7%，远高于其他单倍型；ApoA5基因单倍型与晋汾白猪的初生重、断奶重、6月龄体重、肌内脂肪含量、剪切力和滴水损失均具有明显的相关性，而与pH_{24 h}没有明显的相关性；ApoA5基因Hap 11晋汾白猪的初生重、断奶重、6月龄体重和肌内脂肪含量均最高，且剪切力最低，滴水损失也相对较低；Hap 6的初生重最低；Hap 2的断奶重和6月龄体重均最低；Hap 3的肌内脂肪含量最低，剪切力最高；Hap 10的滴水损失最高。晋汾白猪ApoA5基因Hap 11属于优良性状单倍型，是一个具有优良性状的分子标记，可以用于晋汾白猪的分子育种，提高选种效率。

关键词: 晋汾白猪; ApoA5基因; SNP; 单倍型; 体重; 肉质性状

Abstract: This study was aimed to explore the correlation between the haplotypes of apolipoprotein A5 (ApoA5) gene and body weight,meat quality in Jinfen White pigs.180 Jinfen White pigs were selected,PCR was used to detect the single nucleotide polymorphism (SNP) of ApoA5 gene,and the linkage of these SNPs was analyzed by Haploview software.Non-fully linked SNPs were screened to form the haplotypes of ApoA5 gene,and the frequency of different haplotypes of ApoA5 gene were calculated.The correlation between the haplotypes of ApoA5 gene and body weight,meat quality was analyzed by SPSS software to identify the haplotypes of ApoA5 gene with excellent traits.The results showed that ApoA5 gene of Jinfen White pigs contained 21 SNPs and 5 completely linked regions which included 5,2,3,4 and 2 SNPs,respectively.Ten non-completely linked tag SNPs were selected to form the haplotypes of ApoA5 gene,Hap 1 had the highest gene frequency (46.7%).The haplotypes of ApoA5 gene had a significant correlation with the birth weight,weaning weight,6-month-old body weight,intramuscular fat content,shear force and drip loss,but no significant correlation with pH_{24 h} in Jinfen White pigs.Hap 11 of ApoA5 gene in Jinfen White pigs had the highest birth weight,weaning weight,6-month-old body weight and intramuscular fat content,the lowest shear force,and the lower drip loss.Hap 6 had the lowest birth weight.Hap 2 had the lowest weaning and 6-month-old weight.Hap 3 had the lowest intramuscular fat content and the highest shear force.Hap 10 had the highest drip loss.Hap 11 of ApoA5 gene in Jinfen White pigs had excellent traits,which could be used as a molecular marker to use in molecular breeding of Jinfen White pigs and improve the breeding efficiency.

Key words: Jinfen White pigs; ApoA5 gene; SNP; haplotype; body weight; meat quality

中图分类号:

S813.3

蔡春波, 徐凯, 李萌, 杨阳, 赵燕, 高鹏飞, 郭晓红, 曹果清, 李步高. 晋汾白猪ApoA5基因单倍型与体重和肉质性状的相关性分析[J]. 中国畜牧兽医, 2020, 47(11): 3563-3572.

CAI Chunbo, XU Kai, LI Meng, YANG Yang, ZHAO Yan, GAO Pengfei, GUO Xiaohong, CAO Guoqing, LI Bugao. Correlation Analysis Between Haplotypes of ApoA5 Gene on Body Weight and Meat Quality in Jinfen White Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3563-3572.

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