4个肉牛品种微卫星多态性分析

doi:10.16431/j.cnki.1671-7236.2018.01.021

Abstract

Abstract:

This study was conducted to evaluate the microsatellite polymorphism in the selected 20 microsatellite loci among four beef cattle including Simmental cattle, Limousin cattle, Luxi cattle and Lilu cattle. A total of 144 blood or semen samples from Simmental cattle, Limousin cattle, Luxi cattle and Lilu cattle were collected from different bull stations and the sample numbers were 56, 27, 31 and 30, respectively. The DNA from the blood or semen samples were extracted by Lab-Aid DNA separation kit or salt-extraction method, respectively. The DNA polymorphism of the four beef cattle was analyzed by fluorescence capillary electrophoresis method using the selected 20 microsatellite markers. The genetic variation indices (the number of alleles, polymorphism information content and heterozygosity) and genetic relationship (F-statistic and gene flow) among four beef cattle breeds were analyzed. The results showed that the DNA bands of the beef cattle was neat,and was suitable for the research. A total of 211 alleles were detected in the selected 20 microsatellite loci and the average number of allele in each locus was 10.6. The average observed heterozygosity (Ho) and average expected heterozygosity (He) among the beef cattle breeds were 0.6147 to 0.7176 and 0.6735 to 0.7862, respectively. The polymorphism information content (PIC) of four beef cattle breeds was 0.4853 to 0.8714 in the selected 20 microsatellite loci and the average value of PIC was 0.7284. The average inbreeding coefficient of the four beef cattle was 0.085 and the gene flow in each microsatellite locus was less than 1. In total, the selected 20 microsatellite loci had high polymorphic information, and were effective in the analysis of genetic diversity among different beef cattle breeds.

Key words: beef cattle; microsatellite; polymorphism

CLC Number:

S823.9⁺2

ZHANG Xianglun, YOU Wei, JIN Qing, LIU Xiaomu, TAN Xiuwen, WAN Fachun, CAO Dingguo, LIU Guifen. Analysis of Microsatellite Polymorphism Among Four Beef Cattle Breeds[J]. , 2018, 45(1): 162-170.

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Analysis of Microsatellite Polymorphism Among Four Beef Cattle Breeds

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