拜城油鸡FSHR基因多态性及其与生产性能的关联分析

doi:10.16431/j.cnki.1671-7236.2022.08.020

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the relationship between the polymorphisms of follicle stimulating hormone receptor (FSHR) gene and egg-laying traits,egg quality and hatching performance of Baicheng You chickens,so as to provide new molecular markers for the breeding of high-performance breeders.【Method】 Taking 129 Baicheng You chickens as the research objects,the SNPs were screened by DNA mixed pool resequencing technology,and the related loci were genotyped by Sequenom time-of-flight mass spectrometry technology,and the correlation between the polymorphism of FSHR gene and production performance of Baicheng You chickens was analyzed by the general linear model of SPSS 26.0 software.【Result】 There were two SNPs in exon 1 of FSHR gene:SNP1 (g.7640519 C>A)、SNP2 (g.7640639 T>C),and one SNP in exons 4 and 5:SNP3(g.7692270 C>T) and SNP4(g.7698478 A>G),there were three SNPs in exon 10:SNP5 (g.7716725 G>C),SNP6(g.7715900 A>G) and SNP7 (g.7716470 T>C).With the exception of SNP6,the detection rates were above 90%.Only two genotypes (CC and CA) were present at SNP1,with CC being the dominant genotype,three genotypes (TT,TC,and CC) were present at SNP2,SNP3 and SNP7,three genotypes (AA,AG and GG) were present at SNP4,three genotypes (CC,CG and GG) were present at SNP5. The results of polymorphism analysis showed that the age of first egg of CC genotype at SNP2 was extremely significantly higher than that of TT genotype (P<0.01), and significantly higher than that of CT genotype (P<0.05); The egg weight of TT genotype was significantly higher than that of CT genotype (P<0.05), and the dead embryo rate of setting eggs was significantly higher than that of CC genotype (P<0.05). The shell thickness of TT genotype at SNP3 was significantly higher than that of CC genotype (P<0.05). The total egg mass at 300 days old of CG genotype at SNP5 was extremely significantly higher than that of CC and GG genotypes (P<0.01), the average egg weight at 300 days old and weiht at first egg were significantly higher than that of GG genotype (P<0.05), the body weight at first egg was significantly higher than that of CC genotype (P<0.05), and the shell thickness of GG genotype was significantly lower than that of CC and CG genotypes (P<0.05). The weight at first egg and individual egg weight of TT genotype at SNP7 were significantly higher than that of CC and CT genotypes (P<0.05), the albumen height and yolk weight were significantly higher than that of CT genotype (P<0.05), and the Haugh unit values were extremely significantly higher than that of CC and CT genotypes (P<0.01).【Conclusion】 The CG genotype at SNP5 (g.7716725 G>C) of FSHR gene could be used as the marker genotype that affected the egg-laying performance of Baicheng You chickens,and TT genotype at SNP7 (g.7716470 T>C) of FSHR gene could be used as the dominant genotype that affected the eggs quality of Baicheng You chickens.

Key words: Baicheng You chickens; FSHR gene; polymorphism; production performance

CLC Number:

S813.3

DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.

References

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Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens

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