杜洛克猪HLCS基因多态位点与生长发育性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2020.06.019

Abstract

Abstract: The objective of this study was to clarify the correlation between SNP of holocarboxylase synthetase (HLCS) gene and growth and development traits in Duroc pigs.SNP of HLCS gene was obtained using the sequencing results of Illumina SNP60 chip,the polymorphism information of SNP,the correlation between SNP of HLCS gene and growth and development traits,and the haplotype of SNP were analyzed in this study.The results indicated that 4 SNPs (Ssc13:200455646 T>C,Ssc13:200458655 G>C,Ssc13:200504530 G>T and Ssc13:200551864 T>G) of HLCS gene were detected in Duroc pigs,which were in Hardy-Weinberg equilibrium (P>0.05),showing moderate polymorphism.The correlation analysis results showed that the 100 kg back fat (100 kg BF) and remaining food intake (RFI) of TT genotype in Ssc13:200455646 T>C were significantly higher than that of TC and CC genotypes (P<0.05).The 100 kg BF and RFI of GG genotype in Ssc13:200458655 G>C were significantly higher than that of CG and CC genotypes (P<0.05).The average daily feed intake (ADFI) and average daily gain (ADG) of GT genotype in Ssc13:200504530 G>T were significantly higher than that of TT genotype (P<0.05),the 100 kg age of GT genotype in Ssc13:200504530 G>T were significantly lower than that of TT genotype (P<0.05).The ADFI and ADG of TG genotype in Ssc13:200551864 T>G were significantly higher than that of GG genotype (P<0.05),the 100 kg age of TG genotype in Ssc13:200551864 T>G were significantly lower than that of GG genotype (P<0.05).There was no significant difference of other traits in different genotypes of SNPs (P>0.05).It showed high linkage of Ssc13:200455646 T>C and Ssc13:200458655 G>C,Ssc13:200504530 G>T and Ssc13:200551864 T>G,respectively.The results indicated that there were significant correlation between 4 SNPs of HLCS gene and RFI,ADFI,ADG,100 kg age and 100 kg BF in Duroc pigs,it could lay a foundation for the genetic improvement of growth and development in pigs.

Key words: Duroc pigs; HLCS gene; SNP; growth and development traits

CLC Number:

S813.3

PU Lei, DAI Yuxing, ZHANG Yuebo, ZHANG Longchao, GUO Liang, WANG Lixian. Association Analysis on HLCS Gene Polymorphic Site with Growth and Development Traits in Duroc Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1800-1808.

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Association Analysis on HLCS Gene Polymorphic Site with Growth and Development Traits in Duroc Pigs

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