蛋鸡OCX-32、OVA基因多态性及其与蛋品质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2020.02.023

Abstract

Abstract: This study was aimed to investigate the association between ovocalyxin-32 (OCX-32) and ovalbumin (OVA) genes polymorphism and egg quality traits,and find the molecular genetic markers associated with egg quality traits in laying hens.PCR-SSCP method was used to detect the polymorphism of OCX-32 and OVA genes in the parents’generation of Hyline Brown laying hens.The correlation between different genotypes and the egg quality traits was analyzed by SPSS 22.0 software,and further verified in the offspring to find the dominant genotype.The results showed that three genotypes (AA,BB and CC),two mutation sites located at 7 018 and 7 116 bp were detected in exon 6 of OCX-32 gene,and three genotypes (AA,BC and BB),two mutation sites located at 4 296 and 4 323 bp were detected in exon 5 of OVA gene.The results of association analysis showed that the egg yolk weight of OCX-32 gene AA genotype of parents was extremely significantly higher than BB and CC genotypes (P < 0.01).The Haugh unit and protein height of OVA gene AA genotype of parents were extremely significantly higher than BC genotype (P < 0.01),which was significantly higher than BB genotype (P < 0.05),the albumen weight of AA genotype was significantly higher than BC genotype (P < 0.05).Verification in the offspring confirmed that AA genotype of OCX-32 gene was the dominant genotype for regulating egg yolk weight,and AA genotype of OVA gene was the dominant genotype for regulating Haugh unit,protein height and protein weight.In summary,OCX-32 and OVA genes had certain effects on egg quality traits,which could be used as molecular breeding markers for laying hens.

Key words: Hyline Brown laying hens; OCX-32 gene; OVA gene; polymorphism; egg quality traits

CLC Number:

S813.3

ZHANG Lei, REN Song, ZHANG Lu, SUN Jie. A Polymorphisms of OCX-32 and OVA Genes and Its Association with Egg Quality Traits in Laying Hens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 514-523.

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A Polymorphisms of OCX-32 and OVA Genes and Its Association with Egg Quality Traits in Laying Hens

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