布拉酵母菌海藻糖提取方法的比较研究

›› 2010, Vol. 37 ›› Issue (7): 52-53.

• 生理生化 • Previous Articles Next Articles

The Comparative Study on Methods of Extrating Trehalose from S.boulardii

WANG Zi-hui1,CHU Jie2,WANG Jun-gao1,LIU Ke-chun2

(1.School of Food & Bioengineering, Shandong Institute of Light Industry, Jinan 250353, China;2.Biology Research Institute of Shandong Academy of Science, Jinan 250014, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20
Contact: CHU Jie

Abstract

Abstract: The methods of extracting trehalose from S.boulardii was preliminarily studied in this experiment. Through the contrast between three kinds of extractant and using single and complex treatment，grope the best way to extract trehalose in S.boulardii: extractant is 5% trichloroacetic acid, water bath at 100 ℃ and 30 min after 10 min ultrasonication. Extracting supernatant was hydrolytic and determined glucose with the biosensor and calculate the content of trehalose.

Key words: Saccharomyces boulardii; trehalose; extraction; determination; biosensor

CLC Number:

S881.2

WANG Zi-hui;CHU Jie;WANG Jun-gao;LIU Ke-chun. The Comparative Study on Methods of Extrating Trehalose from S.boulardii[J]. , 2010, 37(7): 52-53.

[1]	JIANG Bowen, LIU Guoshi, ZHANG Lu. Research Progress on the Primary Sex Determination Mechanisms in Mammals [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 614-623.
[2]	LIU Peng, LI Jingjing, QIAO Yanliang, ZHU Lianqin, ZHU Fenghua. Extraction and Structure Characterization of Scutellaria baicalensis Polysaccharide [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2518-2530.
[3]	HE Yue, WANG Hui, CHEN Ruipeng, YU Zhixue, TANG Xiangfang, GUO Yuming, XIONG Benhai. Research Progress on Optical and Electrochemical Biosensors for the Diagnosis of Important Diseases in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 827-837.
[4]	FENG Yanan, YANG Na, MAO Ruoyu, HAO Ya, MA Xuanxuan, TENG Da, WANG Jianhua. Determination of Antimicrobial Peptide NZ2114 in Rat Feed by High Performance Liquid Chromatography [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4463-4472.
[5]	ZHAO Shiyuchen, TANG Siqi, FENG Yali, ZHANG Ying. Research Progress on Virus Detection Methods [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4536-4544.
[6]	PENG Zhiping, ZHANG Jingyan, GUO Zhiting, WANG Lei, ZHANG Kai, ZHANG Kang, WANG Guibo, QIU Zhengying, WANG Xuezhi, LI Jianxi. Extraction and Content Detection of Cordycepin from Cordyceps militaris Residual Medium by Hydrothermal Reflux Method [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3333-3342.
[7]	LI Yipeng, ZHANG Hu, REN Juan, REN Xin, LIU Juxiang, WANG Gengnan. Study on the Extraction Process of Chicoric Acid from Echinacea Root and Its Protective Effects on Oxidative Stress Damaged Intestinal Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2497-2505.
[8]	LI Yahui, LUO Shengjun, TANG Xinggang, YUAN Minggui, WEI Qilin, LOU Hua, XIANG Rong. Study on Optimization of Extraction Process and Antibacterial Activity to Clostridium Perfringens of Total Flavonoids from Vitex negundo L.var. cannabifolia (Sieb.et Zucc.) Hand.-Mazz. [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2406-2413.
[9]	CHEN Yuxiao, LI Huanjuan, XI Xiaohua, HAO Jie, ZHOU Degang, SI Hongbin. Study on Extraction Process of Jiawei Pingwei Oral Liquid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2414-2422.
[10]	LIU Qian, GUO Zhiting, ZHANG Kang, WANG Xuezhi, ZHANG Jingyan, LI Jianxi. Research Progress on Astragalus Polysaccharide on Innate Immunalregulation of Macrophage [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1746-1756.
[11]	LI Xiaoxia, XIAO Hongwei, CAO Pinghua, XU Zhiqian, ZHANG Fang, ZHANG Zhiyang, JING Penghua, YU Xueli, LI Yinghua. Effects of Trehalose on Vitrification of Bovine Immature Oocytes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 224-231.
[12]	CHEN Xuan, LYU Yanqiu, HAN Yue, XU Yichao, JIN Yi. Effect of Trehalose Replacing Partial Glycerol on Frozen-thawed Sperm Quality of Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 256-264.
[13]	YU Jiamin, ZHAO Qian, ZHANG Zhiyan, XU Haiyan, GU Wei. Separation,Purification and Characteristics Analysis of Antibacterial Peptides from Paenibacillus polymyxa [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2830-2837.
[14]	HE Weizhao, ZHANG Huiyan, WANG Hao, ZHAO Qingyu, TANG Chaohua, ZHANG Junmin. Extraction Methods of Yolk Antibody and Its Application in Prevention and Treatment of Bacterial Intestinal Diseases in Livestock and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 640-649.
[15]	GENG Xiang, LIU Xiwang, YANG Yajun, LI Jianyong. Establishment of HPLC Method for Determination of Eprinomectin and Related Substances in Eprinomectin Sustained-release Injection [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4681-4689.

The Comparative Study on Methods of Extrating Trehalose from S.boulardii

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments