Abstract: With the constant variation and spread of viruses, the research on virus detection methods is also developing and deepening.When viral diseases break out, it is particularly important to detect viruses timely and accurately.Traditional virus detection methods include virus isolation and identification, PCR and ELISA, but these methods all have problems such as complicated operation, long detection time and low sensitivity.In recent years, some emerging technologies and optimized methods have been applied to the detection of various viruses, which have brought more accurate and efficient virus detection.For example, the sensitivity of virus detection based on droplet digital PCR can be as much as 16 times higher than Real-time quantitative PCR.There is also a virus detection method based on gene sequencing technology, which attracts more and more attention, it can not only detect the types and quantities of viruses, but also directly and accurately obtain virus gene sequences, and even discover new viruses.In addition, detection technologies based on CRISPR/Cas, biosensors and flow cytometry have also been widely used in the field of virus detection.Although these new technologies have obvious advantages in specificity and sensitivity, they still face some challenges, such as high detection cost and the need for laboratory environment, which limit the popularization and promotion of virus detection technology.Therefore, the future research will focus on improving the accuracy and practicability of detection and reducing the cost, and developing more perfect virus detection technology.The author compares the optimized and emerging detection technology with the traditional detection technology, and summarizes its advantages and disadvantages, in order to provide basis and reference for virus detection and virus disease prevention and control.

Key words: nucleic acid detection; immunological detection; biosensor; point of care testing