内蒙古鼠疫自然疫源地鼠疫菌耐药相关基因检测及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2023.11.021

Abstract

Abstract: 【Objective】 This study was aimed to detect the antibiotic and disinfectant resistance-related genes of Yersinia pestis in Inner Mongolia natural plague foci, and carry out bioinformatics analysis, so as to provide scientific basis for plague prevention and control in this region.【Method】 PCR method was used to detect the streptomycin resistance genes (StrA and StrB), beta-lactam resistance genes (Tem and Ctx-m), sulfanilamide resistance gene (Sul1 and Sul2) and quaternary ammonium salt resistance gene (QacEdelta) in 60 strains of Yersinia pestis isolated from Inner Mongolia natural plague foci.At the same time, the structure, function and antigenic epitopes of StrA, Sul1 and QacEdelta proteins were predicted.【Result】 PCR amplification results showed that the negative and positive controls were established, and the amplification results of 60 strains of Yersinia pestis were negative.No genes related to streptomycin resistance, sulfonamides resistance, beta-lactam resistance and disinfectant resistance were found in the plague strains isolated from Inner Mongolia natural plague foci.Bioinformatics analysis showed that StrA, Sul1 and QacEdelta proteins were all hydrophobic outer membrane proteins that did not contain transmembrane regions, and were secreted proteins.The secondary structure of the three proteins was dominated by alpha helix, which accounted for 37.83%, 45.88% and 46.09%, respectively.Antigen epitope prediction showed that the three proteins contained 9, 8 and 3 B cell antigenic epitopes, and 9, 16 and 14 T cell antigenic epitopes, respectively.【Conclusion】 60 strains of Yersinia pestis isolated from Inner Mongolia plague foci did not have the characteristics of drug resistance and disinfectant resistance related genes.StrA, Sul1 and QacEdelta proteins contained more B and T cell antigenic epitopes and had good antigenicity.The results provided a reference for the rational use of antibiotics and disinfectants in plague prevention and control in these foci.

Key words: Inner Mongolia; Yersinia pestis; disinfectant and drug resistance genes; bioinformatics

CLC Number:

S816.6

WANG Shuyi, LI Jianyun, LIU Fang, FENG Yilan, HAN Bing, FAN Mengguang. Detection and Bioinformatics Analysis of Genes Related to Drug Resistance of Yersinia pestis in Inner Mongolia Natural Plague Foci[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4526-4535.

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Detection and Bioinformatics Analysis of Genes Related to Drug Resistance of Yersinia pestis in Inner Mongolia Natural Plague Foci

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