响应面法优化珠芽蓼多糖提取工艺研究

doi:10.16431/j.cnki.1671-7236.2025.03.042

Abstract

Abstract: 【Objective】 The objective of this experiment was to extract Polygonum viviparum L.polysaccharide by water extraction and alcohol precipitation,and optimize the extraction parameters by response surface methodology,and further analyze the composition of monosaccharides.【Method】 The effects of extraction temperature,liquid-solid ratio,extraction time and ultrasonic power on the extraction yield of Polygonum viviparum L.polysaccharide were investigated by single factor test.With the extraction rate of polysaccharide as the response value,the Box-Behnken experimental design method was used to design the 4-factor and 3-level test and optimize the response surface to determine the relationship between the influencing factors and the extraction rate of polysaccharide.The polysaccharide was deproteinized and purified by Sevag method,and the monosaccharide composition of Polygonum viviparum L.polysaccharide was determined by gas chromatography-mass spectrometry (GC-MS).【Result】 The influencing factors on the extraction rate of Polygonum viviparum L.polysaccharide was extraction temperature ＞ ultrasonic power ＞ extraction time ＞ liquid-solid ratio.The optimal extraction conditions were as follows:Extraction temperature was 50 ℃,liquid-solid ratio was 8∶1,extraction time was 90 min and ultrasonic power was 500 W.Under the optimized conditions,the extraction rate of Polygonum viviparum L.polysaccharide was 6.619%.The results of GC-MS assay showed that the Polygonum viviparum L.polysaccharide was mainly composed of D-arabinose, D-mannose,glucose and D-galactose with a molar ratio of 0.63∶0.57∶6.3∶2.5.【Conclusion】 Response surface methodology was used to optimize the extraction process of Polygonum viviparum L.polysaccharide,and the monosaccharide composition was preliminarily determined.The results provided technical support for the industrial extraction of Polygonum viviparum L.polysaccharide.

Key words: Polygonum viviparum L.; polysaccharides; response surface methodology; extraction

CLC Number:

S853.72

MAN Jingyuan, LIU Yu, XING Xiaoyong, YANG Zhen, HAO Baocheng, LABA Cidan, HE Junfeng, WUJIN Cuomu. Optimization of Extraction Process of Polysaccharides from Polygonum viviparum L.by Response Surface Methodology[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1416-1427.

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Optimization of Extraction Process of Polysaccharides from Polygonum viviparum L.by Response Surface Methodology

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