响应面法模拟优化蜡样芽孢杆菌W-3菌株产木聚糖酶发酵条件

doi:10.16431/j.cnki.1671-7236.2024.03.005

Abstract

Abstract: 【Objective】 This experiment was aimed to optimize the production process of xylanase using response surface methodology,so as to provide a reference basis for further large-scale production.【Method】 Employing single-factor experiments,the influence of six factors,including carbon source,nitrogen source,pH,temperature,inoculum size and agitation speed,on xylanase production by Bacillus cereus (B. cereus) W-3 was examined meticulously.The Box-Behnken response surface optimization design in Design Expert 10.0.3 software was applied.The fermentation conditions were systematically optimized by varying combinations of wheat bran,ammonium chloride,pH,temperature,inoculum size and agitation speed.A mathematical model for enzyme production in relation to these factors was established using a quadratic response surface regression method.The impact levels of each factor on enzyme production were determined through regression analysis.【Result】 The addition of wheat bran,ammonium chloride,pH and temperature were the main effect factors affecting fermentation.Through single-factor optimization,it had been determined that B. cereus W-3 ferments to produce xylanase under the following conditions:Wheat bran concentration of 35 g/L,ammonium chloride concentration of 5 g/L,pH 8.0,a temperature of 70 ℃,an inoculum size of 2%,and an agitation speed of 210 r/min. Having built upon the single-factor optimization,further response surface optimization led to the discovery of superior fermentation parameters.The optimized fermentation conditions included 33.0 g/L wheat bran,5.1 g/L ammonium chloride,pH 8.3,a temperature of 73 ℃,an inoculum size of 2%,and an agitation speed of 210 r/min. Under the optimized fermentation conditions,xylanase enzymatic activity produced by B. cereus W-3 reached 523.24 U/mL.【Conclusion】 In this experiment,the B. cereus W-3 fermentation conditions was optimized by response surface methodology.Under the conditions of 33.0 g/L wheat bran,5.1 g/L ammonium chloride,pH 8.3,73 ℃,2% inoculum size and 210 r/min,a significantly higher xylanase activity of 523.24 U/mL could be obtained,representing a 2.32-fold increase compared to pre-optimization level (225.53 U/mL).

Key words: Bacillus cereus W-3; xylanase; Box-Behnken design; response surface methodology

CLC Number:

S852.61⁺6

XIAO Yao, LI Xuxu, YAN Lihuan, FAN Xinfeng, XUE Zhiquan, JING Xiaoyuan, ZHAO Yan, FENG Yan. Simulation Optimization of Fermentation Conditions for Xylanase Production of Bacillus cereus W-3 by Response Surface Methodology[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 936-944.

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Simulation Optimization of Fermentation Conditions for Xylanase Production of Bacillus cereus W-3 by Response Surface Methodology

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