China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (6): 1765-1772.doi: 10.16431/j.cnki.1671-7236.2020.06.015

• Animal Nutrition and Feed Science • Previous Articles     Next Articles

Optimization of Steam Explosion Pretreated Wheat Straw Using Response Surface Methodology

DU Chunmei, XIONG Benhai   

  1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agriculture Sciences, Beijing 100193, China
  • Received:2019-09-16 Online:2020-06-20 Published:2020-06-20

Abstract: The purpose of this study was to optimize the parameters of steam explosion pretreatment of wheat straw by Box-Behnken response surface method,in order to solve the problem of its low utilization rate.The steam pressure (1.5-2.5 MPa),moisture content (10%-50%) and treatment time (90-210 s) were choosed as the independent variables,the rate of gas production in the in vitro rumen fermentation was the response value,and then the regression model were established and fitted.The results demonstrated that the influence of the treatment time on the rate of gas production in the in vitro rumen fermentation was extremely significant (P<0.01).The effect of steam pressure and moisture content on the rate of gas production in the in vitro rumen fermentation was not significant (P>0.05).According to the F value,the effect of three factors on the rate of gas production in the in vitro rumen fermentation was as treatment time > steam pressure > moisture content.The optimal conditions for steam explosion pretreatment by response surface methodology were steam pressure 1.77 MPa,moisture content 49.91% and treatment time 209.98 s.Under this condition,the rate of gas production in the in vitro rumen fermentation could reach 0.0794 mL/h.The average rate of gas in vitro was 0.0786 mL/h and the relative error was only 1.02%.The wheat straw was treated under the optimal steam explosion condition and subjected to in vitro rumen fermentation.Compared with untreated wheat straw,steam explosion extremely significantly decreased the content of NDF,ADF and hemicellulose in wheat straw (P<0.01),and extremely significantly increased the yield of VFA and NH3-N in vitro rumen incubation (P<0.01).In conclusion,the operational parameter of steam explosion was optimized using the response surface method,which was reasonable and reliable.The steam explosion condition optimized in the present study could improve the nutritional value of wheat straw as forage.

Key words: Box-Behnken design; steam explosion; wheat straw; gas production rate

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