响应面法优化链霉菌产支链蒽醌发酵培养基

doi:10.16431/j.cnki.1671-7236.2024.07.038

Abstract

Abstract: 【Objective】 This study was aimed to optimize the fermentation conditions and medium composition of Streptomyces sp. WS-13394 by response surface methodology,so as to improve the production level of alkylated anthraquinones produced by the strain,and provide technical support for the application of this compound in animal husbandry.【Method】 The optimal shake-flask fermentation conditions and the proper combination of carbon and nitrogen sources and mineral salts for the yield of alkylated anthraquinone produced by Streptomyces sp.WS-13394 were achieved through single-factor experiments.The significant factors affecting the yield of alkylated anthraquinone were fixed through Plackett-Burman design.The optimized medium for the yield of alkylated anthraquinone was achieved through the steepest ascending experiment,response surface design by fitting the significant factors with the yield of alkylated anthraquinone.【Result】 The composition of the optimized medium for the yield of alkylated anthraquinone produced by Streptomyces sp. WS-13394 was as followings:Soluble starch 28.12 g/L,fish meal 10.00 g/L,yeast extract 6.34 g/L,CaCO₃ 0.71 g/L,MgSO₄ 0.50 g/L,and K₂HPO₄ 0.50 g/L. The theoretical yield of alkylated anthraquinone based on the nonlinear regression formula was 408.35 mg/L.The optimal combination was applied to shake bottle fermentation test,and the yield of alkylated anthraquinone was 411.73 mg/L,which was 3.59 times higher than that before optimization (89.70 mg/L).【Conclusion】 Response surface optimization was used to obtain the shaking flask fermentation medium formulation that significantly improved the yield of alkylated anthraquinone produced by Streptomyces sp.WS-13394.The results provided technical support for the studies of the antibacterial activity mechanism of this compound and the evaluation of its effect in animal models.

Key words: Streptomyces sp.; fermentation medium; optimization; response surface methodology; alkylated anthraquinone

CLC Number:

S816.7

LIU Fang, QUAN Tingting, ZHANG Fei, SHI Liqiao, LIU Manli, WU Zhaoyuan. Optimization of Streptomyces sp. Fermentation Medium for Production of Alkylated Anthraquinone by Response Surface Methodology[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3142-3154.

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Optimization of Streptomyces sp. Fermentation Medium for Production of Alkylated Anthraquinone by Response Surface Methodology

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