西藏绒山羊WNT4和HOXC13基因对绒毛纤维直径性状的遗传效应分析

doi:10.16431/j.cnki.1671-7236.2020.02.021

Abstract

Abstract: The aim of this study was to explore the effects of WNT4 and HOXC13 genes polymorphisms on the fiber diameter trait of Tibetan cashmere goats,in order to find out molecular markers related to fiber diameter trait.380 one year old Tibetan cashmere goats were selected as experimental animals.The single nucleotide polymorphism (SNP) of WNT4 and HOXC13 genes were detected by pooled DNA sequencing method,and genotyped by Sequenom MassARRAY genotype technology.The SNPs loci associations with cashmere mean fiber diameter (MFD),fiber diameter standard deviation (FDSD),coefficient of variation of fiber diameter (CVFD) traits were analyzed using the least squares method in the GLM procedure of SAS 9.1 software.The results showed that two SNPs (SNP1 and SNP2) were identified in exon 3 of WNT4 gene,and two SNPs (SNP3 and SNP4) were identified in exon 2 of HOXC13 gene,respectively,the genetic variation was moderate polymorphism (0.25 < PIC < 0.50).The χ² test indicated that the genotypic distributions of SNP1 and SNP2 in WNT4 gene were deviated from Hardy-Weinberg equilibrium (P < 0.05).Association analysis results showed that 4 SNPs was extremely significantly associated with MFD (P < 0.01),SNP2 and SNP3 were extremely significantly associated with FDSD (P < 0.01),SNP2 was extremely significantly associated with CVFD (P < 0.01).In conclusion, WNT4 and HOXC13 genes had significant effects on the fiber diameter in Tibet cashmere goats,so it could be tried to use the SNPs as one of the molecular markers that affected the fiber diameter of Tibet cashmere goats,providing theoretical basis for the breeding of super fine Tibetan cashmere goats.

Key words: Tibetan cashmere goats; WNT4 gene; HOXC13 gene; fiber diameter; SNP; genetic effect

CLC Number:

S813.3

WU Yujiang, FU Xuefeng, SUO Langda, BA Gui, DE Ji, CI Rendeji, SONG Tianzeng, TIAN Kechuan. A Genetic Effects Analysis of WNT4 and HOXC13 genes on Fiber Diameter Trait in Tibetan Cashmere Goats[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 497-505.

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A Genetic Effects Analysis of WNT4 and HOXC13 genes on Fiber Diameter Trait in Tibetan Cashmere Goats

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