澳洲白绵羊美丽臀和肌肉生长抑制素基因基因型的检测

Abstract

Abstract: Callipyge (CLPG) and myostatin (MSTN) are recognized as the growth of muscle-related genes in the world,which produce "beautiful buttocks " and "double-muscle" good traits after mutation. To study whether imported AWF sheep carrying mutations in two genes,we used PCR-RFLP technique to check. The results showed that there was a C→T SNP in CLPG gene,AWF sheep populations had CC and CT genotypes;A G→A SNP locus in the 3'-UTR region of MSTN gene, AWF sheep populations had GG and GA genotypes. Analysis of variance with the R language between different genotypes and growth in different gender,MSTN and CLPG of different genotypes on the AWF rams weight,height,length,rump width,fat thickness and loin area were no significant differences (P>0.05);CLPG CT genotype on ewe weight was significantly higher than CC genotype (P<0.05),in length was very significantly higher than CC genotype (P<0.01),MSTN GA genotype on ewe loin area was very significantly higher than GG genotype (P<0.01). We detected contain CLPG and MSTN mutations in imported AWF sheep from Australia,and the mutation of sheep body traits had significant effects.Therefore,the AWF sheep could used as improved local sheep breeds,gene pyramiding and terminals supporting male in ternary system after the introduction.

Key words: AWF sheep; CLPG; MSTN; PCR-RFLP

CLC Number:

S813.3

ZHANG Shu-zhen, ZHANG Li, ZHAO Fu-ping, WANG Hui-hua, LIU Rui-zao, LIU Zhen, FAN Hong-ying, WEI Cai-hong, DU Li-xin. Detection of CLPG and MSTN Genes Genotypes on AWF Sheep[J]. , 2014, 41(10): 172-178.

References

1 李云龙,杨章平,毛永江,等.DLK1 基因的单核苷酸多态性与绵羊生长发育性状的相关性分析[J]. 中国畜牧杂志,2011,47(1):18~20.
2 刘铮涛,李祥龙,冯敏山,等. MSTN基因内含子2多态性与山羊体重性状相关研究[J].畜牧兽医学报,2006,37(8):745~748.
3 孟详人,马月辉,关伟军,等. 4个绵羊品种双肌臀基因基因型的检测[J]. 中国畜牧兽医,2009,36(4):119~121.
4 Clop A,Marcq F,Takeda H,et al. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep[J]. Nat Genet,2006,38(7):813~818.
5 Cockett N E,Jackson S P,Shay T L,et al. Chromosomal localizationof the callipyge gene in sheep (Ovis aries) using bovine DNA markers[J]. Proc Natl Acad Sci USA,1994,91(8):3019~3023.
6 Cockett N E,Jackson S P,Shay T L,et al. Polar overdominance at the ovine callipyge locus[J]. Science,1996,273(5272):236~238.
7 Dunner S,Miranda M E,Amigues Y,et al. Haplotype diversity of the myostatin gene among beef cattle breeds[J].Genet Sel Evol,2003,35(1):103~108.
8 Freking B A,Murphy S K,Wylie A A,et al. Identification of the single base change causing the callipyge muscle hypertrophy phenotype,the only known example of polar overdominance in mammals[J]. Genome Res,2002,12(10):1496~1506.
9 Gill J L,Bishop S C,McCorquodale C,et al. Associations between the 11-bp deletion in the myostatin gene and carcass quality in angus-sired cattle[J].Animal Genetics,2009,40(1):97~100.
10 Jackson S P,Miller M F,Green R D. Phenotypic characterization of rambouillet sheep expression the callipyge gene:Ⅲ. Muscle weights and muscle weight distribution[J]. Anim Sci,1997,75:133~138.
11 Lee S J,Mcpherron A C. Myostatin and the control of skeletal muscle mass[J]. Curr Opin Genet Dev,1999,9(5):604~607.
12 Leymaster K A,Freking B A. Genetic variations and associations for improving meat production and meat qualities in sheep and goats[A]. The 6th World Congr Genet Appl Livest Prod[C].1998.
13 Mcpherron A C,Lawler A M,Lee S J. Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member[J]. Nature,1997,387(6628):83~90.
14 Meyer H H,Haribaskar S,Abdulkhaliq A M,et al. Callipyge gene effects on lamb growth,carcass traits,muscle weights and meat characteristics[A]. The 6th World Congr Genet Appl Livest Prod[C].1998.
15 Wiener P,Woolliams J A,Frank-Lawale A,et al. The effect of a mutation in the myostatin gene on meat and carcass quality[J]. Meat Science,2009,83(1):127~134.

[1]	WANG Jing, ZHU Zhe, ZHANG Peng, BI Yanzhen. Site-directed Mutagenesis of Porcine Myostatin Gene Using Single Base Editor [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2880-2887.
[2]	FU Yongzhi, MA Xiaojun, WANG Maohan, LI Zhe, CHEN Zhiyuan, QUAN Wei, ZHAO Han, SU Yongmei, WU Jianhua, ZHU Wenjin. Bioinformatics Analysis and Tissue Expression of MSTN Gene in Yangyuan Donkeys [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2910-2919.
[3]	GAO Beiyao, LIU Yanguang, JIA Qi, LIU Jianqiang, LUO Xinhui, ZHANG Lichun. Study on Polymorphism and Differential Expression of PBD-124 Gene in Different Breeds of Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2208-2215.
[4]	ZHANG Junxia, HE Na, LI Mingming, LYU Cailing, CUI Yajie, LANG Xia, WANG Cailian. Cloning,Bioinformatics and Tissue Expression Analysis of MSTN Gene in Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4307-4317.
[5]	ZHANG Lei, LI Danni, WANG Yuchen, YANG Lichun, HE Jing, SONG Yuxuan. Polymorphism of MSTN Gene and Its Association with Growth Traits in East Friensian Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4537-4544.
[6]	JIANG Yixuan, WANG Yaling, XING Shanshan, SONG Guohua, XU Huifen, LI Ming. Cloning, Bioinformatics Analysis and Expression Profile Construction of MSTN Gene in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3501-3511.
[7]	SHENG Hui, GUO Yiwen, ZHANG Linlin, TAN Haoyun, HU Debao, LI Xin, DING Xiangbin, GUO Hong. Effect of MSTN Gene on Actin Cytoskeleton Regulatory Pathway [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3554-3564.
[8]	ZHU Zhe, ZHANG Jianying, HUA Zaidong, REN Hongyan, XIAO Hongwei, ZHANG Liping, ZHENG Xinmin, BI Yanzhen, LU Shengsheng. A Effects of Myostatin on the Expression of MMP-2/7/9 in PK15 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 345-351.
[9]	SHENG Hui, ZHANG Linlin, GUO Yiwen, LI Xin, DING Xiangbin, GUO Hong. A Selection of Reference Genes for Real-Time Quantitative PCR in Bovine Skeletal Muscle Satellite Cells Interfering with MSTN [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 381-391.
[10]	ZHU Feifei, ZHANG Junxing, ZHANG Linlin, LI Xin, LIU Xinfeng, GUO Yiwen, DING Xiangbin. A Effect of Interfering with MSTN on the Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 479-487.
[11]	XUE Linli, LI Yilei, HOU Yaqin, JI Yunyan, REN Huawei, GENG Jianjun, WANG Haidong, HE Xiaoyan, XING Haiyun. Expression of Myostatin in Myocardium of Mice at Different Developmental Stages [J]. , 2018, 45(6): 1479-1485.
[12]	GUO Ziru, ZHANG Li, MA Yunlong, SU Xiaohu, ZHOU Huanmin, ZHANG Yanru, LIU Xiao. Establishment of MSTN Gene Knockout in Mongolia Bovine Cell Lines Mediated by CRISPR-Cas9 System [J]. , 2018, 45(4): 841-849.
[13]	WANG Yan-qin, SHAO Yong-gang. Study on the Association Relationship Between the Polymorphism of MSTN Gene and Production Performance in Baicheng Fatty Chicken [J]. , 2017, 44(4): 1054-1060.
[14]	YI Heng-jie, LI Hui, ZHAO Zhong-hai, YANG Hua-ting, PENG Bang-xing, WANG Yu. The Polymorphisms of MSTN Gene and Its Relationship with Meat Quanlity Traits in Gaopo Pig [J]. , 2017, 44(4): 1102-1107.
[15]	LI Wei, ZU Pan-yu, LI Hong-lin, LIN Jia-dong, ZHANG Fu-ping. Polymorphisms of MSTN Gene and Its Association with Meat Quality Traits in Chishui Black-bone Chicken [J]. , 2017, 44(4): 1115-1121.

Detection of CLPG and MSTN Genes Genotypes on AWF Sheep

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments