肌肉生长抑制素在小鼠不同发育阶段心肌组织中的表达

doi:10.16431/j.cnki.1671-7236.2018.06.007

Abstract

Abstract:

The aim of this experiment was to investigate the expression of myostatin (MSTN) gene in mouse myocardium at different developmental stages.12 healthy mice at different developmental stages were chosen,including young (7 days old),sexual mature (21 to 28 days old), somatic mature (42 to 56 days old) and aged mice (84 days old or older),with three mice per developmental stage.The expression of MSTN gene in mice myocardium and myocardial development were detected by quantitative Real-time PCR,HE staining and immunohistochemistry.The results of quantitative Real-time PCR showed that MSTN gene could express in myocardium tissue of mice at all different developmental stages.And it was the highest in aged mice and the lowest in somatic mature mice.Compared with the aged mice,the expression of MSTN gene in juvenile mice and sexually mature mice was significantly decreased (P<0.05), however,it was extremely significantly decreased in somatic mature mice (P<0.01).The results of HE staining showed that the growth of myocardial nuclei and muscle fibers were differences in different ages.Among them,the nucleus was the most and the muscle fiber arrangement was relatively close in juvenile mice,but the indexes of myocardial development in sexually mature mice,somatic mature mice and aged mice were decreased in turn.Immunohistochemical results showed that MSTN was mainly expressed in the nucleus of myocardium,and the level of its expression in each stage was consistent with the results of quantitative Real-time PCR.

Key words: myostatin (MSTN); myocardium; expression distribution

CLC Number:

XUE Linli, LI Yilei, HOU Yaqin, JI Yunyan, REN Huawei, GENG Jianjun, WANG Haidong, HE Xiaoyan, XING Haiyun. Expression of Myostatin in Myocardium of Mice at Different Developmental Stages[J]. , 2018, 45(6): 1479-1485.

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Expression of Myostatin in Myocardium of Mice at Different Developmental Stages

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