China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (2): 506-513.doi: 10.16431/j.cnki.1671-7236.2020.02.022

• Genetics and Breeding • Previous Articles     Next Articles

A Expression Analysis and Target Gene Prediction of miR-206 in Jinmao Black Chickens

HUANG Jinxing1,2, CHEN Lan1,2, ZHANG Tao1,2, CHEN Fuxiang1,2, WU Pengfei1,2, MAO Jing3, GONG Yongshuang3, XIE Kaizhou1,2, WANG Jinyu1,2, DAI Guojun1,2   

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. International Joint Laboratory for Agricultural and Agricultural Safety, Ministry of Education, Yangzhou 225009, China;
    3. Jiangsu Sandeli Animal Husbandry Development Limited Company, Changzhou 213200, China
  • Received:2019-06-06 Published:2020-02-28

Abstract: The objective of this study was to study the tissue expression distribution of miR-206 and its expression pattern in skeletal muscle growth,and predict its possible mechanism of action.Eight tissues of heart,liver,spleen,lung,kidney,breast muscle,leg muscle and abdominal fat were collected from 4-week-old Jinmao Black chickens.Breast muscle and leg muscle were collected from 2,6,10,14 and 16-week-old Jinmao Black chickens.The expression of miR-206 in eight tissues and skeletal muscle at different growth stages using Real-time PCR.The target genes of miR-206 were predicted and conducted to function annotation by bioinformatics methods.The results showed that the expression level of miR-206 in breast muscle and leg muscle was extremely significantly higher than that in other tissues (P < 0.01).miR-206 was lowly expressed in abdominal fat,heart,liver,spleen,lung and kidney,and specifically expressed in skeletal muscle.The expression pattern showed that the expression of miR-206 was the highest in leg muscle and breast muscle at 2-weeks-old,and showed a downward trend in general.Target gene prediction and functional analysis revealed that miR-206 had 356 target genes,21 of which were involved in myogenesis.Multiple target genes were known to be involved in the regulation of myogenesis,including paired box 7 (Pax7),insulin-like growth factor 1 (IGF1),brain-derived neurotrophic factor 1 (BDNF1),retinoic acid receptor beta (RARB) and frizzled class receptor 7 (FZD7) genes.Pathway analysis showed that four pathways involved in myogenesis were significantly enriched,including MAPK signaling pathway,Wnt signaling pathway,regualtion of actin cytoskeleton and focal adhesion pathway.Combined with the expression results,we speculated that miR-206 might participate in the regulation of chicken muscle myogenesis by targeting Pax7、IGF1、BDNF1、RARB, FZD7 genes and MAPK,Wnt,regulation of actin cytoskeleton and focal adhesion pathways.This study revealed the tissue distribution and expression pattern of miR-206 in the growth of chicken and predicted its possible function and mechanism in the regulation of skeletal muscle myogenesis,which was the important foundation and basis for further study of the mechanism of action and expression regulation of miR-206.

Key words: miR-206; Jinmao Black chickens; tissue expression distribution; expression pattern; target gene

CLC Number: