China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (5): 1489-1497.doi: 10.16431/j.cnki.1671-7236.2020.05.023

• Genetics and Breeding • Previous Articles     Next Articles

Cloning and Bioinformatics Analysis of CYP19A1 Gene in Taihang Chickens

ZHANG Hui1, ZHANG Qingyang1, LI Chenchen1, YANG Junqi1, DU Yunliang1, LI Kaiyang2, LIU Yufang1   

  1. 1. College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056001, China;
    2. Beijing General Station of Animal Husbandry, Beijing 100107, China
  • Online:2020-05-20 Published:2020-05-18

Abstract: This study was aimed to clone and analyze the CYP19A1 gene in laying hens by bioinformatics and investigate its expression in different tissues and egg production.The Taihang chicken in Hebei was taken as the research object.The CYP19A1 gene CDS region was cloned and sequenced by designing primers,and its functional structure was predicted by bioinformatics software.The results showed that the chicken CYP19A1 gene contained a 1 509 bp open reading frame (ORF) encoding 502 amino acids.The results of homology alignment and phylogenetic tree analysis indicated that the sequence of the Taihang chicken CYP19A1 gene was close to that of duck (90.7%).The physicochemical properties of CYP19A1 protein were predicted and analyzed.The structure formula of CYP19A1 protein was C2602H4103N675O719S36 and the molecular mass was 57.51 ku.The theoretical isoelectric point was 5.99,with leucine being the highest abumdance.The CYP19A1 was hydrophilic protein.There was portions that could form alpha helix (58.95%),extended chain (2.18%),beta sheet (3.93%) and random coils (34.93%).The results of tissue expression profiling showed that the gene was expression in all the tissues,with the highest abundance in ovary.The results of Real-time PCR showed that the expression level of CYP19A1 gene in ovary issure of high-yield group was significantly higher than that of low- yield group (P<0.05).The above results provided important research data for studying the laying performance of the Taihang chicken.

Key words: Taihang chicken; CYP19A1 gene; bioinformation analysis; expression pattern

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