太行鸡CYP19A1基因克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2020.05.023

Abstract

Abstract: This study was aimed to clone and analyze the CYP19A1 gene in laying hens by bioinformatics and investigate its expression in different tissues and egg production.The Taihang chicken in Hebei was taken as the research object.The CYP19A1 gene CDS region was cloned and sequenced by designing primers,and its functional structure was predicted by bioinformatics software.The results showed that the chicken CYP19A1 gene contained a 1 509 bp open reading frame (ORF) encoding 502 amino acids.The results of homology alignment and phylogenetic tree analysis indicated that the sequence of the Taihang chicken CYP19A1 gene was close to that of duck (90.7%).The physicochemical properties of CYP19A1 protein were predicted and analyzed.The structure formula of CYP19A1 protein was C₂₆₀₂H₄₁₀₃N₆₇₅O₇₁₉S₃₆ and the molecular mass was 57.51 ku.The theoretical isoelectric point was 5.99,with leucine being the highest abumdance.The CYP19A1 was hydrophilic protein.There was portions that could form alpha helix (58.95%),extended chain (2.18%),beta sheet (3.93%) and random coils (34.93%).The results of tissue expression profiling showed that the gene was expression in all the tissues,with the highest abundance in ovary.The results of Real-time PCR showed that the expression level of CYP19A1 gene in ovary issure of high-yield group was significantly higher than that of low- yield group (P<0.05).The above results provided important research data for studying the laying performance of the Taihang chicken.

Key words: Taihang chicken; CYP19A1 gene; bioinformation analysis; expression pattern

CLC Number:

S813.3

ZHANG Hui, ZHANG Qingyang, LI Chenchen, YANG Junqi, DU Yunliang, LI Kaiyang, LIU Yufang. Cloning and Bioinformatics Analysis of CYP19A1 Gene in Taihang Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1489-1497.

References

[1] GHOSH D,GRISWOLD J,ERMAN M,et al.Structural basis for androgen specificity and oestrogen synthesis in human aromatase[J].Nature,2009,457(7226):219-223.
[2] JING R,GU L,LI J,et al.A transcriptomic comparison of theca and granulosa cells in chicken and cattle follicles reveals ESR2 as a potential regulator of CYP19A1 expression in the theca cells of chicken follicles[J].Comparative Biochemistry and Physiology.Part D,Genomics & Proteomics,2018,27:40-53.
[3] AZCOITIA I,YAGUE J G,GARCIASEGURA L M.Estradiol synthesis within the human brain[J].Neuroscience,2011,191(4):139-147.
[4] BADER M I,REITMAYER C,ARNDT C,et al.Age dependency of estrogen responsiveness in the uterus and adipose tissue of aromatase-knockout (ArKO) mice[J].Journal of Steroid Biochemistry and Molecular Biology,2012,128(1-2):0-37.
[5] HAURI H A,MEYER B M,LANG M M,et al.Aromatase deficiency owing to a functional variant in the placenta promoter and a novel missense mutation in the CYP19A1 gene[J].Clinical Endocrinology,2011,75(1):39-43.
[6] GENNARI L,MERLOTTI D,NUTI R.Aromatase activity and bone loss[J].Advances in Clinical Chemistry,2011,54:129-164.
[7] MAIA H,HADDAD C,CASOY J.Correlation between aromatase expression in the eutopic endometrium of symptomatic patients and the presence of endometriosis[J].International Journal of Women's Health,2012,4(1):61-65.
[8] 景若曦.鸡卵泡体细胞转录组研究及芳香化酶基因调控分析[D].武汉:华中农业大学,2018. JING R X.Transcriptomic analysis of somatic cells in chicken follicle get insight to potential regulator of CYP19A1 expression[D].Wuhan:Huazhong Agricultural University,2018.(in Chinese)
[9] ECHTERNKAMP S E,AAD P Y,EBORN D R,et al.Increased abundance of aromatase and follicle stimulating hormone receptor mRNA and decreased insulin-like growth factor-2 receptor mRNA in small ovarian follicles of cattle selected for twin births[J].Journal of Animal Science,2012,25:1-11.
[10] MONTSERRAT N.Effects of follicle stimulating hormone on estradiol-17beta,production and P-450 aromatase (CYP19) activity and mRNA expression in brown trout vitellogenin ovarian follicles in vitro[J].General and Comparative Endocrinology,2004,137(2):123-131.
[11] RAHAIE A,TOGHYANI M,EGHBALSAIED S.Cotreatment of IGF1 and fadrozole upregulates the expression of RSPO1,SOX9,and AMH in chicken embryos[J].Cells Tissues Organs,2019,255(5043):467-470.
[12] RUOXI J,LANTAO G,JINQIU L,et al.A transcriptomic comparison of theca and granulosa cells in chicken and cattle follicles reveals ESR2,as a potential regulator of CYP19A1,expression in the theca cells of chicken follicles[J].Comparative Biochemistry & Physiology Part D Genomics & Proteomics,2018,27:40.
[13] WANG J,GONG Y.Transcription of CYP19A1 is directly regulated by SF-1 in the theca cells of ovary follicles in chicken[J].General and Comparative Endocrinology,2017,247:1-7.
[14] 孙甜甜,杨祝良,邓继贤,等.广西麻鸡CYP19A1基因的克隆、序列分析和组织表达谱研究[J].中国家禽,2017,39(18):60-62. SUN T T,YANG Z L,DENG J X,et al.Cloning,sequence analysis and tissue expression profile of CYP19A1 gene in Guangxi Ma chicken[J].China Poultry,2017,39(18):60-62.(in chinese)
[15] 包海声,蔡晗,韩伟,等.CYP19A1基因在中华鳖早期卵巢分化中的功能研究[J].中国科学:生命科学,2017,47(6):640-649. BAO H S,CAI H,HAN W,et al.Functional characterization of CYP19A1 in female sexual differentiation in pelodiscus sinensis[J].Scientia Sinica(Vitae),2017,47(6):640-649.(in Chinese)
[16] 赵丹.雌激素和TGF-β1对鸡原始卵泡和生长卵泡发育的调节作用及其机理的研究[D].杭州:浙江大学,2018. ZHAO D.Effects of estrogen and TGF-β1 on development of chicken primordial growing follicles[D].Hangzhou:Zhejiang University,2018.(in Chinese)
[17] SIMPSON E R,MAHENDROO M S,MEANS G D,et al.Aromatase cytochrome P450,the enzyme responsible for estrogen biosynthesis[J].Endocrine Reviews,1994,15(3):342-355.
[18] 何珊,常宏,刘大艳,等.补肾调周法对超排卵周期颗粒细胞CYP19A1 mRNA表达的影响[J].中国医院用药评价与分析,2015,15(7):849-852. HE S,CHANG H,LIU D Y,et al.Effects of reinforcing kidney and menstrual cycle regulation on the expression of CYP19A1 mRNA in granulocytes in controlled ovarian hyperstimulation cycle[J].Evaluation and Analysis of Drug-use in Hospitals of China,2015,15(7):849-852.(in Chinese)
[19] ZHAO H,INNES J,BROOKS D C,et al.A novel promoter controls Cyp19a1 gene expression in mouse adipose tissue[J].Reproductive Biology & Endocrinology,2009,7(1):37.
[20] HALM S,KWON J Y,RANDWEAVER M,et al.Cloning and gene expression of P45017 alpha-hydroxylase,17,20-lyase cDNA in the gonads and brain of the fathead minnow Pimephales promelas[J].General & Comparative Endocrinology,2003,130(3):256-266.
[21] 田晓东,徐日福,车永顺,等.鸭CYP19A1基因mRNA组织表达特性研究[A] 全国畜禽遗传标记研讨会[C].2010. TIAN X D,XU R F,CHE Y S,et al.Study on mRNA expression of duck CYP19A1 gene[A].National Symposium on Animal Genetic Markers[C].2010.(in Chinese)
[22] 唐永凯,李建林,陈文华,等.奥利亚罗非鱼卵巢芳香化酶基因的克隆及其表达[J].中国水产科学,2008,15(5):729-737. TANG Y K,LI J L,CHEN W H,et al.Cloning and expression of P450arom from Oreochromis aureus ovary[J].Journal of Fishery Sciences of China,2008,15(5):729-737.(in Chinese)
[23] 朱晓亮,徐桂云,杨宁,等.利用shRNA真核表达载体干涉雌性鸡胚性腺中芳香化酶基因(CYP19A1)的表达[J].农业生物技术学报,2011,19(6):1150-1156. ZHU X L,XU G Y,YANG N,et al.Interference of polypeptide gene (CYP19A1) expression by shRNA eukaryotic expression vector in female chick embryo gonads[J].Journal of Agricultural Biotechnology,2011,19(6):1150-1156.(in Chinese)

Cloning and Bioinformatics Analysis of CYP19A1 Gene in Taihang Chickens

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WEI Jiarong, ZHANG Beibei, MA Tenghe, LI Mengxiao, LI Xuenan, WANG Bin, FU Guanhua, WANG Hongna, LIU Chao. Cloning and Identification of Splice Isoforms of CCNB2 Gene and Their Expression Patterns in Follicular Development in Taihang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3726-3735.
[2]	TIAN Yuan, LONG Feng, LI Anqi, CHEN Jiayue, YANG Sen, ZAN Linsen, CHENG Gong. Amplification, Sequence Feature and Expression Pattern of Snail2 Gene in Qinchuan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3147-3157.
[3]	HUANG Jinxing, CHEN Lan, ZHANG Tao, CHEN Fuxiang, WU Pengfei, MAO Jing, GONG Yongshuang, XIE Kaizhou, WANG Jinyu, DAI Guojun. A Expression Analysis and Target Gene Prediction of miR-206 in Jinmao Black Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 506-513.
[4]	MU Songyin, XIONG Xianrong, MA Hongcheng, HAI Zhuo, LI Jian. Cloning of L-PGDS Gene and Its Expression Pattern in Different Tissues and Testis at Different Development Stages in Cattle-yak [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3985-3992.
[5]	GUO Xiaohong, LIU Cuicui, LI Meng, ZHANG Wanfeng, QIN Benyuan, LIU Min, CAI Chunbo, GAO Pengfei, CAO Guoqing, LI Bugao. Expression Profile and Patterns of CREB-H Gene in Different Tissues and Liver of Pig [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2543-2550.
[6]	ZHENG Jie, LIU Yufang, MA Lu, XUE Ming. Genetic Structure and Expression Pattern Analysis of TCHH in Tan Sheep [J]. , 2019, 46(6): 1585-1593.
[7]	LI Peiwei, WU Sen, WANG Yaning, WANG Hongbao, ZAN Linsen. Expression Patterns of PLIN2 Gene in Subcutaneous Adipose Tissue and Association Analysis of Single Nucleotide Polymorphisms with Meat Quality Traits in Qinchuan Cattle [J]. , 2018, 45(6): 1580-1589.
[8]	LU Xing-rong, LI Zhi-Peng, SUN Jun-ming, LV Ling-yan, XU Zhong-feng, LIU Qing-you, SHI De-shun, CUI Kui-qing. Effect of DNA Methylation Inhibitor 5-Aza-CdR on Patterns of Pluripotent-related Genes and Methylation Genes in Handmade Cloning Embryo [J]. , 2016, 43(9): 2240-2248.
[9]	DU Feng-jiao, LIU Xiao-lin, WU Zhu-lian, LI Xiao-kai, QU Chun-feng, ZHAO Xin, LEI Chuan, RUAN Qiu-yan, LU Feng-hua, SHI De-shun. Cloning and Sequence Analysis of Buffalo BDNF Gene and Investigation of its Expression Pattern in Different Tissues [J]. , 2015, 42(4): 830-837.
[10]	ZHU Peng, SU Xiao-ping, SU Jie, LIU Jin-feng, LIU Qing-you, SHI De-shun. Cloning and Expression Pattern Investigation of Swamp Buffalo ALK3 Gene [J]. , 2015, 42(3): 516-524.
[11]	JIA Li-li, JIN Feng, FU Shao-yin, ZHENG Zhu-qing, DU Chen, JI Xiao-yang, WANG Zhi-xin, LI Jin-quan, LIU Shao-qing, ZHANG Wen-guang. Comparation of Expression Pattern of Clock Genes in Cashmere Goat Skin [J]. , 2015, 42(2): 251-257.
[12]	LIN Yu-shu, JIANG Heng-jie, YANG Feng-shuo, CUI Kui-qing, SHI De-shun, LIU Qing-you. Cloning,Bioinformatics and Expression Pattern Analysis of Buffalo MC1R Gene [J]. , 2015, 42(10): 2529-2537.
[13]	GOU Hua，HU Shen-qiang，PAN Zhi-xiong，LIU He-he，LI Liang，WANG Ji-wen. Developmental Expression Patterns of VLDLR and ApoER2 Genes in Duck Muscles [J]. , 2014, 41(3): 7-13.
[14]	ZHANG Ran, LIN Yu-shuang . Study on Evolutionary and Expression Pattern of Cardiac α-actin in Cattle [J]. , 2013, 40(8): 46-49.
[15]	ZHANG Xiao-ning, LU Jian, REN Hang-xing, WEI Cai-hong, ZHNAG Li, ZHAO Fu-ping, DU Li-xin. Developmental Expression Patterns of AKT Gene in Fetal Longissimus Dorsi Muscle between Texel and Ujumqin Sheep during the Second Half of Gestation [J]. , 2012, 39(8): 20-25.