DNA甲基化酶抑制剂5-Aza-CdR对多能基因及甲基化相关基因在徒手克隆胚胎中表达模式的影响

doi:10.16431/j.cnki.1671-7236.2016.09.004

Abstract

Abstract:

The expression pattern of pluripotent gene Oct4 and Nanog,and methylation related genes Dnmt1 and Tets of handmade cloning (HMC) embryos were studied by Real-time quantitative PCR assay.The effect of genes expression pattern by 5-Aza-CdR on HMC reconstructed embryos was also explored. The results showed that the expression of Oct4,Nanog and Tet3 genes reached peak on 2-cell stage,the expression of Dnmt1 and Tet2 genes declined with the embryo development,while the expression of Tet1 gene increased.The use of 5-Aza-CdR didn't change the expression pattern of Oct4,Tet1 and Tet3 genes,but increased the expression of Nanog gene at the beginning of embryo development,while decreased the expression of Dnmt1 and Tet2 genes.The expression pattern of Oct4,Nanog,Dnmt1 and Tets genes in the development of HMC embryo was established,the use of 5-Aza-CdR could influence the methylation process of HMC embryo.

Key words: HMC; 5-Aza-CdR; gene; expression pattern

CLC Number:

S814.6

LU Xing-rong, LI Zhi-Peng, SUN Jun-ming, LV Ling-yan, XU Zhong-feng, LIU Qing-you, SHI De-shun, CUI Kui-qing. Effect of DNA Methylation Inhibitor 5-Aza-CdR on Patterns of Pluripotent-related Genes and Methylation Genes in Handmade Cloning Embryo[J]. , 2016, 43(9): 2240-2248.

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Effect of DNA Methylation Inhibitor 5-Aza-CdR on Patterns of Pluripotent-related Genes and Methylation Genes in Handmade Cloning Embryo

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