沼泽型水牛ALK3基因克隆分析与表达模式研究

doi:10.11843/j.issn.1671-7236.2015.03.002

Abstract

Abstract: Clone,sequence bioinformatics analysis and expression pattern determination were performed of ALK3 gene in swamp buffalo.A pair of special primers was designed according to the released sequence of bovine ALK3 in GenBank.ALK3 gene was amplified by RT-PCR,its gene sequence character and protein structure was systemically analyzed by bio-informatics techniques.The expression pattern of buffalo ALK3 in different tissues was also assayed with QRT-PCR.The results showed that buffalo ALK3 gene fragment including a 1 599 bp whole length CDS (coded 532 amino acids) was cloned and sequenced.The sequence multi-aligned results showed that buffalo ALK3 gene shared 98%,96%,95%,93%,94% and 91% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa,Equus caballus,Homo sapiens and mice,respectively.ALK3 protein sequence analysis showed that it was weakly alkaline,had signal peptide,positioned on the membrane,and with the presence of S_TKc and GS (GS motif) structure.In addition,the expression pattern analysis results showed that buffalo ALK3 expressed in all 15 tested samples,with the most abundant expression in ovary,followed by hypophysis,lung and testis,the minimal expression in cumulus cells was observed.The cloning and analysis of ALK3 gene provided an important foundation for further study regulation mechanism of ALK3 gene in buffalo in the future.

Key words: buffalo; ALK3; cloning analysis; expression pattern

CLC Number:

S832.8⁺3

ZHU Peng, SU Xiao-ping, SU Jie, LIU Jin-feng, LIU Qing-you, SHI De-shun. Cloning and Expression Pattern Investigation of Swamp Buffalo ALK3 Gene[J]. , 2015, 42(3): 516-524.

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Cloning and Expression Pattern Investigation of Swamp Buffalo ALK3 Gene

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