猪肉和鸡肉中脱氧雪腐镰刀菌烯醇和T-2毒素酶联免疫吸附检测方法的建立

doi:10.11843/j.issn.1671-7236.2015.03.003

Abstract

Abstract: To protect animal-origin foods,an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to detect deoxynivalenol (DON) and T-2 toxin in pork and chicken,respectively.The results showed that the limit of detection (LOD) of DON in pork and chicken were 34.9 and 43.5 μg/kg,respectively,and the recoveries ranged from 72.7% to 97.1% with the coefficients of variation (CV) lower than 8.7%.Besides,the LOD of T-2 toxin in pork and chicken were 33.7 and 28.7 μg/kg,respectively,and the recoveries ranged from 72.1% to 95.0% with CV less than 11.3%.The developed method had the advantage of being sensitive and accurate,and was suitable for the rapid detection of DON and T-2 residue in pork and chicken.

Key words: enzyme-linked immunosorbent assay (ELISA); deoxynivalenol (DON); T-2 toxin; pork; chicken

CLC Number:

R155.5

SONG Xiao-li, LIU Mei-xuan, LUO Xiang-shu, LI Cheng-long, YANG Shu-peng, WANG Zhan-hui, ZHANG Su-xia. Development of an Enzyme-linked Immunosorbent Assay for the Detection of Deoxynivalenol and T-2 Toxin in Pork and Chicken[J]. , 2015, 42(3): 525-530.

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Development of an Enzyme-linked Immunosorbent Assay for the Detection of Deoxynivalenol and T-2 Toxin in Pork and Chicken

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