›› 2011, Vol. 38 ›› Issue (1): 88-91.

• 生物技术 • Previous Articles     Next Articles

Establishment and Rudiment Application of Duplex PCR for Goatpox Virus and Orf Virus

XIANG Zhi-long1，CHENG Zhen-tao1，ZHUO Jian-hua3，ZHOU Bi-jun2，OU De-yuan2，XIAN Si-mei1，LIU Fang1，RAN Guang-xin1   

  1. (1.College of Animal Scence,Guizhou University，Guiyang 550025,China；2.Insitute of Animal Disease，Guizhou Province，Guiyang,550025, China；3.The Bureau of Livestock and Veterinary of Jingshan Country，Hubei Province，Hubei 431800,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01
  • Contact: OU De-yuan;XIAN Si-mei

Abstract: Two pairs of primers were designed according to the sequences of ITR gene of Goatpox virus (GPV) and H2L gene orf virus(ORFV) available in GenBank，respectively.Nucleic acids of GPV and ORFV were mixed as template, a duplex PCR assay was developed based on optimization of reaction conditions. Using the duplex PCR assay，the specific fragments 289 bp and 507 bp were amplified respectively from DNA samples of GPV and ORFV，but not amplified from foot-mouth disease virus，fowl pox virus，bluetongue virus and skin from normal healthy goat. The sensitivity of the duplex PCR reached 4 pg for GPV DNA and 0.4 pg for ORFV DNA，respectively. 12 clinical material were amplified by the duplex PCR assay，detection rate of GPV was 25%(3/12)，detection rate of ORFV was 75%(9/12).The results showed that this duplex PCR assay was rapid，sensitive and specific for simultaneous diagnosis or differentiation of GPV and/or from ORFV infections.

Key words: goatpox virus; orf virus; duplex PCR; detection

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