›› 2011, Vol. 38 ›› Issue (1): 81-87.

• 生物技术 • Previous Articles     Next Articles

Isolation of Porcine Encephalomyocarditis Virus GXLC Strain and Analysis of the Molecular Characteristics of its 3D Gene

CHEN Jin-xi1,SHI Kai-chuang2,HUANG Sheng-bin2,LU Wen-jun2,QU Su-jie2,ZHENG Min2,LI Jun2   

  1. (1. Qinzhou Center for Animal Disease Control and Prevention,Qinzhou 535000,China;2. Guangxi Center for Animal Disease Control and Prevention,Nanning 530001,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01
  • Contact: SHI Kai-chuang


The clinical tissue samples collected from piglets died of suspected encephalomyocarditis were homogenated and the supernatant was inoculated into baby hamster kidney (BHK-21) cell lines to isolate encephalomyocarditis virus (EMCV). According to the cytopathic effect (CPE) and the results of RT-PCR and indirect fluorescent antibody test (IFA),one strain of EMCV was successfully isolated and designated as GXLC strain. The 3D gene of GXLC strain was amplified by RT-PCR,cloned into pMD18-T vector,sequenced and analyzed. The sequence analysis showed that the 3D gene of GXLC strain consisted of 1380 nucleotides encoding 460 amino acids and contained 7 epitopes. The homology analysis indicated that the homology of 3D gene of GXLC strain and other EMCV strains available in GenBank were from 84.7% to 99.7% and from 96.1% to 99.6% based on the nucleotide and deduced amino acide sequences,respectively. The phylogenetic tree based on the 3D gene nucleotide sequences revealed that all the EMCV strains could be divided into two groups: group Ⅰ and group Ⅱ,and group Ⅰ could be subdivided into subgroup Ⅰa and subgroup Ⅰb. The strains from pig distributed in group I and group Ⅱ,while the strains from mouse distributed in group I and the strains from human distributed in group Ⅱ. GXLC strain,and other EMCV isolates from China belonged to subgroup Ⅰa.

Key words: swine; encephalomyocarditis virus; isolation; 3D gene; molecular characteristic

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