九疑山兔FGF10基因克隆、生物信息学及组织表达分析

doi:10.16431/j.cnki.1671-7236.2025.03.003

Abstract

Abstract: 【Objective】 The aim of this study was to explore the molecular structural characteristics of fibroblast growth factor 10 (FGF10) gene in Jiuyishan rabbits and its expression levels in different tissues of Jiuyishan rabbits at different ages.【Method】 The longissimus dorsi muscle of Jiuyishan rabbit was used as material in the experiment to clone the coding region sequence (CDS) of FGF10 gene in Jiuyishan rabbits.The amino acid sequence similarity of FGF10 was analyzed with MegAlign software，and a phylogenetic tree was constructed using Mega 11.0 software.The physicochemical properties,secondary structure,and tertiary structure of the FGF10 protein were predicted and analyzed using online tools like ProtParam and SOPMA.The expression of FGF10 gene in heart,liver,spleen,lung,kidney and longissimus dorsi muscle of 1,35,84 and 180 days old Jiuyishan rabbits was detected by Real-time quantitative PCR.【Result】 The length of CDS of FGF10 gene in Jiuyishan rabbits was 663 bp,which encoded 220 amino acids.The similarity of its encoded amino acid sequence with Oryctolagus cuniculus，Lepus europaeus，Gallus domestiaus,Pelobates fuscus，Ovis aries，Bos taurus，Equus caballus，Homo sapiens and Mus musculus by 100.0%,99.5%,87.3%,73.1%,97.2%,97.2%,99.1%,99.0% and 93.8%,respectively.The results of the phylogenetic tree showed that the genetic relationship between Jiuyishan rabbits and Equus caballus was relatively close. FGF10 protein of Jiuyishan rabbits was characterized as an hydrophilic unstable protein.It was characterized by the presence of a transmembrane region and a signal peptide.The structural domains of the FGF10 protein included a transmembrane region,a low-complexity region,and a homologous domain that was typical of the FGF family,in addition to two sites designated for glycosylation and 40 sites for phosphorylation.Its secondary structure was predominantly composed of random coils (49.55%),with extended chains making up another 25.91%.The predicted tertiary structure aligned with the secondary structure.Protein interaction predictions revealed that FGF10 protein interacted with KL,FGFR2,FGFR4,and other proteins.Real-time quantitative PCR results revealed that the expression of FGF10 gene at four stages was the highest in lung,followed by kidneys,and the lowest in liver.In addition,the expression of FGF10 gene in longissimus dorsi muscle showed a downward trend with time,and reached the lowest level at 84 days of age.【Conclusion】 FGF10 protein from Jiuyishan rabbits had the highest similarity with Oryctolagus cuniculus and Lepus europaeus and was an unstable hydrophilic protein with transmembrane regions,signal peptides,glycosylation sites,and phosphorylation sites. FGF10 gene was differentially expressed in different ages and tissues of Jiuyishan rabbits.These findings provided important reference for further studying the potential role of FGF10 gene in muscle development.

Key words: Jiuyishan rabbits; FGF10 gene; cloning; molecular characteristic; spatiotemporal expression spectrum

CLC Number:

S829.1

HUANG Nianni, HUANG Zike, LI Congcong, LIU Han, MA Shiyu, SONG Guo, JIANG Liang, PENG Weibo, YANG Lianxi, HUANG Shengqiang. Cloning,Bioinformatics and Tissue Expression Analysis of FGF10 Gene in Jiuyishan Rabbits[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1001-1010.

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Cloning,Bioinformatics and Tissue Expression Analysis of FGF10 Gene in Jiuyishan Rabbits

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