›› 2011, Vol. 38 ›› Issue (1): 76-80.
• 生物技术 • Previous Articles Next Articles
MENG Hui,WEN Kai,SHEN Jian-zhong,WANG Zhan-hui,ZHANG Su-xia
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Abstract: In this study, we constructed a phage display antibody library specific for gentamicin (GEN) to get the anti-getamicin single chain antibody(scFv). We used the anti-gentamicin hybridoma (2A3) as a source of gene to produce full-length scFv fragment. The full-length scFv fragment was ligated into the phagemid vector pCANTAB5E. Then the phagemid was transformed to E.coli TGl cells by electroporation. The size of antibody libraries was 6.5×106. The primary phage display library was riched by stabilized antigen immunoaffinity panning with GEN-OVA conjugates. 9 clones congtained insert and showed GEN-positive in phage-ELISA, which lay the foundation for further immunological analysis.
Key words: gentamicin; phage display antibody library; single chain antibody
CLC Number:
R392
MENG Hui;WEN Kai;SHEN Jian-zhong;WANG Zhan-hui;ZHANG Su-xia. Construction of Anti-gentamicin Phage Display scFv Libraries,Selection and Identification[J]. , 2011, 38(1): 76-80.
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