Abstract: In this study, we constructed a phage display antibody library specific for gentamicin (GEN) to get the anti-getamicin single chain antibody(scFv). We used the anti-gentamicin hybridoma (2A3) as a source of gene to produce full-length scFv fragment. The full-length scFv fragment was ligated into the phagemid vector pCANTAB5E. Then the phagemid was transformed to E.coli TGl cells by electroporation. The size of antibody libraries was 6.5×106. The primary phage display library was riched by stabilized antigen immunoaffinity panning with GEN-OVA conjugates. 9 clones congtained insert and showed GEN-positive in phage-ELISA, which lay the foundation for further immunological analysis.

Key words: gentamicin; phage display antibody library; single chain antibody