噬菌体展示系统的构建及其在疾病防控领域应用研究进展

doi:10.16431/j.cnki.1671-7236.2022.05.010

Abstract

Abstract: As the most widely used antibody display technology,phage display technology has gradually become an important tool for the production of genetically engineered antibodies.Phage display technology is a molecular biology technology that uses phage or phagemid as a carrier to integrate exogenous polypeptide genes into phage genes and display the exogenous protein on the surface of the phage in the form of fusion expression.In recent years,phage display technology has been used more and more widely in the field of antibody screening.Compared with traditional methods for preparing antibodies,phage display technology has the characteristics of high throughput,low cost,and simple operation.And the antibodies screened by this technology can not only be selected and purified with the assistance of the tag protein,but also the complete gene sequence of the single-chain antibody can be obtained by the method of gene sequencing.This review first classified the phage antibody library.According to the source of the antibody,the phage antibody library could be divided into natural antibody library and immune antibody library.The specificity and positive rate of antibody libraries prepared from immunized animals were significantly higher than those of natural antibody libraries.Then the construction process of the phage antibody library was briefly described.The selection of the phage expression vector was the key to the display technology.The helper phage integrated with the phagemid gene infected its specific strains,so that the system could be selectively screened through antibiotic plates.Finally,the research progress of the application of phage display technology in the field of disease prevention and control was discussed.The first humanization of antibodies had made it possible for homologous antibodies to be used in clinical applications.Subsequent research and development of animal homologous antibodies for the prevention and treatment of viral diseases had proved the ability of phage display technology to produce diagnostic or potential therapeutic reagents.This review mainly focused on the construction of phage display system and its application in the field of disease prevention and control,in order to guide the subsequent screening of single-chain antibodies through this technology.

Key words: phage display technology; fusion expression; single chain antibody; phagemid; disease prevention and control

CLC Number:

HOU Wei. Research Progress on Construction of Phage Display System and Its Application in the Field of Disease Prevention and Control[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1688-1696.

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Research Progress on Construction of Phage Display System and Its Application in the Field of Disease Prevention and Control

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