Abstract: CAPN1 gene as the major gene which controls beef tenderization was concerned by researchers. In this study，we reported the 4685 bp loci genotype frequency and allele frequency in Chinese Red Steppe，Yanbian Yellow cattle, Red Angus×Simmental, Limousin×Simmental, Germany Yellow cattle×Simmental and Charolais×Simmental cross. The results showed that genotype frequency and allele frequency were significant different in these population. As the predominant genotype in Yanbian Yellow cattle，the frequency of CT genotype was 0.53，the frequency of CC and TT genotype just were 0.27 and 0.20 respectively. The TT and CT genotype had very high frequency in Germany Yellow cattle×Simmental cross, reached 0.50 and 0.40 respectively, the CC genotype frequency only was 0.10, the T allele frequency reached 0.70, and the C allele frequency only was 0.30. The others cross cattle had similar genotype and allele frequency to Yanbian Yellow cattle. The most interesting result in this study was the genotype distribution in Chinese Red cattle，we only found one genotype TT which suggested C allele was not exist in Chinese Red cattle. The complement of genotype frequency test of 4685 bp loci in CAPN1 gene in triple cattle laid a foundation to research the relationship of 4685 bp loci to meat tenderness.

Key words: Chinese Red cattle; Yanbian Yellow cattle; cross cattle; CAPN1; PCR-SSCP