›› 2010, Vol. 37 ›› Issue (2): 104-107.

• 遗传繁育 • Previous Articles     Next Articles

SNP Detection of Exon 2 of PRL Gene and its Associationswith Egg-laying and Broodiness Traits in Shiqiza Chicken

CAO Ding-guo，LI Fu-wei，LEI Qiu-xia，HAN Hai-xia，ZHOU Yan，
LI Gui-ming，HUANG Bao-hua, LU Yan   

  1. (Institute of Poultry，Shandong Academy of Agricultural Sciences，Jinan 250023，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

Abstract: Abstract: Exon 2 of prolactin gene were detected by PCR-SSCP and the DNA fragments were sequenced．The correlation analysis between the polymorphism of the exon 2 and egg-laying and broodiness traits were carried out respectively．The results indicated that there were two polymorphic sites in exon 2．A C/T mutation at the position of the 3838 bp was found，which resulted in 3 genotypes(AA，AB and BB)．Furthermore，an amino acid changed from Leucine to Phenylalanine. The frequencies of genotype AA and allele A were the highest．The least square analysis showed that the first broodiness day of BB type chickens was later than AA and AB type chickens (P<0.01)， the times of broody of BB chickens were more than AA and AB type(P<0.05). But there was no significant difference in egg production among the 3 genotypes．

Key words: Key words: prolactin gene; PCR-SSCP; Shiqiza; broodiness traits