水貂黑素皮质素受体4基因部分序列的克隆测序

Abstract

Abstract: Abstract：Melanocortin receptor-4(MC4R)gene in mink was cloned and sequenced，in order to provide theoretical foundation for further research of the corelevantion of MC4R gene in mink and fat traits，and location. MC4R gene of mink was amplified using particular primers，cloned by polymerase chain reaction (PCR) method and sequenced，and spliced by using DNAMAN. The length of MC4R gene in mink was 816 bp. The sequences had been sent to GenBank and registered. Comparison of the fragment sequence with that of other mammal animals MC4R gene was carried out through BLAST in GenBank，the results showed that the homologies of the nucleotide sequence with river otter，dog，fox，panda，river horse，boar，goat，human and rat were 89% to 95%，MC4R gene of mink was successfully cloned in this study，indicating it was quite conservative among species.

Key words: Key words：melanocortin receptor-4 gene; cloning and equencing; sequence analysis; mink

LIU Zong-yue;YANG Hong-yan;BAI Xiu-juan. Cloning and Sequencing of Melanocortin Receptor-4 Gene of Mink[J]. , 2010, 37(2): 101-103.

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Cloning and Sequencing of Melanocortin Receptor-4 Gene of Mink

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