关木通中马兜铃酸A的提取及含量测定

Abstract

Abstract: Extracted aristolochic con-acid by dehydrate alcohol as the solvent from 150 g manchurian dutchmanspipe stem powder,get crude extract after rotary evaporation and vacuum dehydration，and dissolved extractum with NaHCO₃ saturated solution，adjusted the pH to 3 after delamination.Used silica gel G chromatographic colum to laminar analysis. The eluent was made up of chloroform∶acetidin∶methylbenzol(2∶1∶1).Then get flav-crystal 0.389g after recrystallization, identified and assayed the content with R-HPLC. the result showed that the flav-crystal was aristolochic acid A monomer, and the content reached 99.2%. The methold of extraction could get high purity aristolochic acid A,and it was a high performance way.

Key words: manchurian dutchmanspipe stem; aristolochic acid A; extraction; assaying

CLC Number:

R927.2

WANG Dian-ren;WANG Yan-ying;LU Yan;SHEN Hong;ZHANG Zhong-wen;WU Guo-juan. The Aristolochic Acid A’s Abstraction in Manchurian Dutchmanspipe Stem and Contet Assaying[J]. , 2008, 1(4): 147-149.

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The Aristolochic Acid A’s Abstraction in Manchurian Dutchmanspipe Stem and Contet Assaying

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