摩拉水牛和尼里-拉菲水牛PRKAA2基因SNPs检测及遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2018.05.019

Abstract

Abstract:

In order to study the polymorphism of protein kinase AMP-activated catalytic subunit alpha 2 (PRKAA2),the genome DNA of Murrah and Nili-Ravi buffaloes were used as templates to amplify exon 4 and intron 3 partial sequences of PRKAA2 gene,the SNPs were detected by routine sequencing,and the genetic diversity was analyzed.The results showed that there was 1 SNP (c.462 G>A) in exon 4 and 3 SNPs (IVS3.557 T>C,IVS3.560 C>T and IVS3.565 G>A) in the intron 3.The genetic diversity results showed that wild homozygote and heterozygote were more advantages than mutant homozygote at c.462 G>A,the mutation homozygous genotypes were non-dominant genotypes at IVS3.557 T>C and IVS3.560 C>T,and the heterozygous genotypes were dominant genotypes at IVS3.565 G>A.IVS3.565 G>A in Murrah buffaloes and c.462 G>A in Nili-Ravi buffaloes were in non-equilibrium.All of 4 SNPs in Murrah buffaloes were at moderate polymorphism,c.462 G>A and IVS3.557 T>C in Nili-Ravi buffaloes were at low polymorphism,and IVS3.560 C>T and IVS3.565 G>A were at moderate polymorphism.The heterozygosity of IVS3.557 T>C was lower in two buffalo populations.The genotype frequency and gene frequency of IVS3.565 G>A in Murrah buffalo and c.462 G>A in Nile-Rafi buffalo were imbalanced,the genetic variation of IVS3.557 T>C in Nile-Rafi buffalo was low,without a high selection potential.4 SNPs would be combined into 5 haplotypes,T-C-G-G type was the dominant haplotype in two buffalo populations.In conclusion,4 SNPs of PRKAA2 gene in Murrah and Nili-Rafi buffaloes could provide a reference for marker-assisted selection breeding.

Key words: Murrah buffalo; Nili-Ravi buffalo; PRKAA2 gene; SNP; routine sequencing

CLC Number:

S813.3

HUANG Yuemeng, ZHENG Haiying, YANG Chunyan, HUANG Jiaxiang, YAN Shengfei, LI Shulu, YU Nongqi, LI Mengqi, SHANG Jianghua. SNPs Detection and Genetic Diversity Analysis of PRKAA2 Gene in Murrah and Nili-Ravi Buffaloes[J]. , 2018, 45(5): 1274-1282.

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SNPs Detection and Genetic Diversity Analysis of PRKAA2 Gene in Murrah and Nili-Ravi Buffaloes

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