静原鸡ELOVL2基因遗传多态性研究

doi:10.16431/j.cnki.1671-7236.2018.03.022

Abstract

Abstract:

In order to study the genetic characteristics and nucleotide polymorphism sites of elongation of very-long-chain fatty acids 2 (ELOVL2) gene in Jingyuan chicken,the polymorphisms of ELOVL2 gene were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequence.The results showed that there was no polymorphism of ELOVL2 gene exon 6,there were moderate polymorphism of introns 2 and 6 in Jingyuan chicken.The total of 5 genotypes(A₂A₂,B₂B₂,C₂C₂,D₂D₂ and A₂C₂),4 alleles(A₂,B₂,C₂ and D₂) and 3 SNPs loci were detected on the intron 2 amplified fragment of ELOVL2 gene.The 3 SNPs were g.63372228+4918G > A transformation,g.63372228+5024T > C transformation and g.63372228+4928C > A transversion.A₂ was the dominant allele (0.62),and A₂A₂ was the dominant genotype (0.54).The total of 3 genotypes (A₁A₁,B₁B₁ and A₁B₁) and 2 alleles (A₁ and B₁) were detected on E2e6I6 amplified fragments.A₁ was the dominant allele (0.67),A₁A₁ was the dominant genotype (0.54),and only allele B₁ had a g.63388000+748G > C transversion.Population genetics analysis showed that genetic homozygosity of ELOVL2 gene introns 2 and 6 in Jingyuan chicken were higher and deviation from Hardy-Weinberg equilibrium (P < 0.05).The results showed that the mutations of the ELOVL2 gene introns 2 and 6 in the Jingyuan chicken were high,which were moderate polymorphism,showing the homozygous advantage and no mutation on the exon 6.

Key words: Jingyuan chicken; ELOVL2 gene; polymorphism

CLC Number:

S813.3

MU Tong, GU Yaling, ZHAO Ping, FENG Xiaofang, CHEN Jiaping, CHEN Yafei, LIU Liyuan, JIANG Qiufei, ZHANG Juan. Study on Polymorphism of ELOVL2 Gene in Jingyuan Chicken[J]. , 2018, 45(3): 730-737.

References

[1] 李维,祖盘玉,李洪林,等.赤水乌骨鸡肌肉生长抑制素基因多态性及其与肉质性状的关联分析[J].中国畜牧兽医,2017,44(4):1115-1121. LI W,ZU P Y,LI H L,et al.Polymorphisms of MSTN gene and its association with meat quality traits in Chishui Black-bone chicken[J].China Animal Husbandry & Veterinary Medicine,2017,44(4):1115-1121.(in Chinese)
[2] 顾亚玲,张娟,何生虎.静原鸡品种选育与饲养管理[M].宁夏:阳光出版社,2017. GU Y L,ZHANG J,HE S H.Breed Selection and Feeding Management in Jingyuan Chicken[M].Ningxia:Sunshine Press,2017.(in Chinese)
[3] ZHANG J,ZHAO S,LEI Z,et al.Identification of polymorphisms in the bovine paraoxonase 1 gene[J].Journal of Animal & Veterinary Advances,2013,12(2):229-233.
[4] NAGANUMA T,SATO Y,SASSA T,et al.Biochemical characterization of the very long-chain fatty acid elongase ELOVL7[J].FEBS Letters,2011,585(20):3337-3341.
[5] LEONARD A E,PEREIRA S L,SPRECHER H,et al.Elongation of long-chain fatty acids[J]. Progress in Lipid Research, 2004,43(1):36.
[6] JAKOBSSON A,WESTERBERG R,JACOBSSON A.Fatty acid elongases in mammals:Their regulation and roles in metabolism[J].Progress in Lipid Research,2006,45(3):237-249.
[7] GUILLOU H,ZADRAVEC D,MARTIN P G,et al.The key roles of elongases and desaturases in mammalian fatty acid metabolism:Insights from transgenic mice[J].Progress in Lipid Research,2010,49(2):186-199.
[8] ÓSCAR MONROIG,ROTLLANT J,CERDÁ-REVERTER J M,et al.Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio,early embryonic development[J]. Biochimica et Biophysica Acta,2010,1801(10):1145.
[9] LEONARD A E,KELDER B,BOBIK E G,et al.Identification and expression of mammalian long-chain PUFA elongation enzymes[J].Lipids,2002,37(8):733-740.
[10] KOBAYASHI T,ZADRAVEC D,JACOBSSON A.ELOVL2 overexpression enhances triacylglycerol synthesis in 3T3-L1 and F442A cells[J].Febs Letters,2007,581(17):3157.
[11] WANG Y,BOTOLIN D,CHRISTIAN B,et al.Tissue-specific,nutritional,and developmental regulation of rat fatty acid elongases[J].Journal of Lipid Research,2005,46(4):706-715.
[12] BOTOLIN D,WANG Y,CHRISTIAN B,et al.Docosahexaneoic acid (22:6,n-3) regulates rat hepatocyte SREBP-1 nuclear abundance by Erk and 26S proteasome-dependent pathways[J].Journal of Lipid Research,2006,47(1):181-92.
[13] 李响,丁珍,吴义霞,等.ELOVL2基因多态性与乳母乳汁DHA水平的关联性分析[J].吉林大学学报(医学版),2015,41(6):1230-1234. LI X,DING Z,WU Y X,et al.Association analysis on polymorphisms of ELOVL2 gene and DHA level in breast milk of lactating mothers[J].Journal of Jilin University (Medical Edition),2015,41(6):1230-1234.(in Chinese)
[14] 郭鹏程,戴珊珊,杨浩然,等. ELOVL5基因遗传变异与中国荷斯坦奶牛乳质性状的关联分析及功能验证[J].中国兽医学报,2017,37(4):741-745. GUO P C,DAI S S,YANG H R,et al.elovl5 gene variants reveal significant effects of milk quality in holstein cows[J].Chinese Journal of Veterinary Science,2017(4):741-745.(in Chinese)
[15] TANAKA T,SHEN J,ABECASIS G R,et al.Genome-wide association study of plasma polyunsaturated fatty acids in the InCHIANTI study[J].PLoS Genetics,2009,5(1):e1000338.
[16] ILLIG T,GIEGER C,ZHAI G,et al.A genome-wide perspective of genetic variation in human metabo-lism[J].Nature Genetics,2010,42(2):137-141.
[17] LEMAITRE R N,TANAKA T,TANG W,et al.Genetic loci associated with plasma phospholipid n-3 fatty acids:A meta-analysis of genome-wide association studies from the CHARGE consortium[J].PLoS Genetics,2011,7(7):e1002193.
[18] 邵丹,王来娣,张蕊,等.鹅肥肝形成相关基因的研究进展[J].中国畜牧兽医,2012,39(9):42-46. SHAO D,WANG L D,ZHANG R,et al.Research progress of genes related to fatty liver formation in goose[J].China Animal Husbandry & Veterinary Medicine,2012,39(9):42-46.(in Chinese)
[19] 孙晓艳.小鼠elovl2与△6 desaturase及线虫sfat1三基因共转促进EPA向DHA转化的研究[D].西安:陕西师范大学,2012. SUN X Y.Co transfection of mouse elovl2 and △6 desaturase and sfat1 of nematode promote the transformation of EPA to DHA[D].Xi'an:Shaanxi Normal University,2012.(in Chinese)
[20] 母童,张娟,赵平,等.静原鸡ELOVL2和ELOVL5基因表达的组织特异性研究[J].浙江农业学报,2017,29(8):1290-1296. MU T,ZHANG J,ZHAO P,et al.Tissue-specific expression analysis of ELOVL2 and ELOVL5 genes in Jingyuan chicken[J].Acta Agriculturae Zhejiangensis,2017,29(8):1290-1296.(in Chinese)
[21] 夏德佳,孙琳,史杰萍,等.多不饱和脂肪酸代谢限速酶基因多态性与吉林省汉族偏执型精神分裂症的病例-对照研究[J].卫生研究,2011,40(2):154-157. XIA D J,SUN L,SHI J P,et al.A case-control study between gene polymorphisms of polyunsaturated fatty acids metabolic rate-limiting enzymes and paranoid schizophrenia of Han ethnicity in Jilin province[J].Journal of Hygiene Research,2011,40(2):154-157.(in Chinese)
[22] 邢华苑,王颜刚.ELOVL6基因rs9997926位点多态性与2型糖尿病病人胰岛素抵抗的相关性[J].青岛大学医学院学报,2017,53(1):84-86. XING H Y,WANG Y G.Correlation between ELOVL6 gene rs9997926 polymorphism and insulin resistance in type 2 diabetes[J].Acta Academiae Medicinae Qingdao Universitatis,2017,53(1):84-86.(in Chinese)
[23] 尉阳,王蕾蕾,金镇.ELOVL6基因单核苷酸多态性与妊娠期糖尿病的相关性[J].解剖科学进展,2015,21(2):171-173. WEI Y,WANG L L,JIN Z.Association between single nucleotide polymorphism of ELOVL6and gestational diabetes mellitus[J].Progress of Anatomical Sciences,2015,21(2):171-173.(in Chinese)
[24] LI X,GAN Z W,DING Z,et al.Genetic variants in the ELOVL5 but not ELOVL2 gene associated with polyunsaturated fatty acids in Han Chinese breast milk[J].Biomedical and Environmental Sciences,2017,30(1):64-67.

Study on Polymorphism of ELOVL2 Gene in Jingyuan Chicken

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.
[2]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[3]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[4]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[5]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[6]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[7]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[8]	DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.
[9]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[10]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[11]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[12]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[13]	ZHANG Yonghao, XIAO Yi, GUO Lijun, Tuerxun·Abudoureheman, WANG Yutao. Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 962-972.
[14]	LI Mingming, WU Qingwei, DENG Yuting, JIN Shengwei, HE Na, ZHANG Junxia. Correlation Analysis of BMPRⅡ Gene Polymorphism and Its Haplotype with Lambing Traits in Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 587-597.
[15]	WANG Yingtong, YANG Suan, TAN Yuancheng, WANG Chunyuan, ZHANG Yiyu. Polymorphism of SLC8A1 Gene and Its Effect on Eggshell Quality in Changshun Blue-eggshell Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4688-4696.