季节对新疆牛源E.coli O157:H7分布的影响研究

doi:10.16431/j.cnki.1671-7236.2018.01.027

Abstract

Abstract:

To study the impact of season to the distribution of bovine E.coli O157:H7,samples of anus swabs (399),feces (68),water (29) and feed (43) were collected in the spring, summer,autumn and winter from A,B and C farms of Xinjiang. After enrichment by EC broth, SMAC and MUG selective culture were then performed. Finally,PCR was used for identification and virulence gene detection of isolated strains. A total of 5 E.coli O157:H7 strains were isolated from 539 samples from three farms (0.93%,5/539), 2 of them were from spring (1.44%,2/139),1 from autumn(0.56%,1/180),2 from winter (1.38%,2/145) and no strains were isolated from summer samples. One strain were isolated from anus swab samples in farm B (0.69%,1/145) and one were isolated from anus swabs (0.66%,1/152) and three strains were isolated from feed samples in farm C (20.00%,3/15),and no target strains were isolated from water samples. The distribution of bovine E. coli O157:H7 had obvious seasonal characteristics.One E.coli O157:H7 strain of farm B was isolated from autumn and four of farm C were from isolated spring (2 strain) and winter (2 strain),and the isolation rate of E. coli O157:H7 in spring and winter were higher than that in summer and autumn. In conclusion,under the special climate characteristics and feeding mode in Xinjiang,to prevent and control the spreading of E. coli O157:H7 of cattle,we must pay great attention on hygiene management of pens at cold season, specially avoiding the feed contaminated by feces.

Key words: bovine; season; E.coli O157:H7; isolation and identification

CLC Number:

S852.61

SU Zhanqiang, WANG Dong, ZHANG Jinyu, ZHANG Yi, LIU Yingyu, MA Kaiqi, GAO Jiaojiao, SUN Xue, YAO Gang. Study on Seasonal Impact to the Distribution of Bovine E.coli O157: H7 in Xinjiang[J]. , 2018, 45(1): 212-218.

References

[1] DAY N P,SCOTLAND S M,CHEASTY T,et al. Escherichia coli O157:H7 associated with human infections in the United Kingdom[J].The Lancet,1983,1(8328):825.
[2] 王燕,谢贵林, 杜琳.大肠杆菌O157:H7感染流行概况[J].微生物学免疫学进展,2008,36(1):51-58. WANG Y,XIE G L,DU L. The prevalence of Escherichia coli O157:H7 infection[J].Progress in Microbiology and Immunology,2008,36(1):51-58.(in Chinese)
[3] 叶青,史明坤,刘波,等. 河北省O157:H7大肠杆菌病原学和流行病学研究[J].中国公共卫生,2000,16(7):613-614. YE Q,SHI M K,LIU B,et al. Etiology and epidemiology of Escherichia coli O157:H7 in Hebei province[J]. Chinese Journal of Public Health,2000,16(7):613-614.(in Chinese)
[4] 李洪卫,景怀琦,逄波,等.徐州市2000年肠出血性大肠埃希菌O157:H7感染性腹泻的调查[J].中华流行病学杂志,2002,23(2):119-122. LI H W, JING H Q,PANG B,et al. Study on diarrhea disease caused by enterohemorrhagic Escherichia coli O157:H7 in Xuzhou city,Jiangsu province in 2000[J].Chinese Journal of Epidemiology,2002,23(2):119-122.(in Chinese)
[5] 韦小瑜,游旅,田克诚,等. 贵州省首次从腹泻病例中检出肠出血性大肠杆菌O157:H7[J].中国人兽共患病学报,2015,31(2):183-185. WEI X Y,YOU L,TIAN K C,et al.Isolation and identification of the strain of enterohemorrhagic Escherichia coli O157:H7 isolated from a patient with infectious diarrhea in Guizhou province,China[J].Chinese Journal of Zoonoses,2015,31(2):183-185. (in Chinese)
[6] BACH S J,MCALLISTER T A,VEIRA D M,et al. Transmission and control of Escherichia coli O157:H7——A review[J].Canadian Journal of Animal Science,2002,82(4):475-490.
[7] KEEN E C. Paradigms of pathogenesis:Targeting the mobile genetic elements of disease[J]. Frontiers in Cellular & Infection Microbiology,2012,2(2):161.
[8] COBBAUT K,BERKVENS D,HOUF K,et al. Escherichia coli O157 prevalence in different cattle farm types and identification of potential risk factors[J]. Journal of Food Protection,2009,72(9):1848-1853.
[9] 郭维植,林成水,程发稷.福建省家畜、家禽O157大肠杆菌初查[J].中国人兽共患病杂志,1998,2:3-6. GUO W Z,LIN C S,CHENG F J,et al. Preliminary investigation on enteuohemorrhagic Escherichia coli O157 from domestic animals and fowl in Fujian province[J].Chinese Journal of Zoonoses,1998,2:3-6.(in Chinese)
[10] 龚云伟,王平,董伟力.长春地区家禽(家畜)中E.coli O157:H7调查[J].微生物学杂志,2006,26(6):102-104. GONG Y W,WANG P,DONG W L,et al. Investigation on E.coli O157:H7 among poultry and livestock in Changchun area[J]. Journal of Microbiology,2006,26(6):102-104.(in Chinese)
[11] 赵璐. 新疆不同地区牛粪源大肠杆菌O157:H7分离鉴定及毒性研究[D].乌鲁木齐:新疆农业大学,2014. ZHAO L.To detect the dung E.coli O157:H7 and toxicity studies in different regions of Xinjiang[D].Urumqi:Xinjiang Agricultural University,2014. (in Chinese)
[12] BESSER R E,LETT S M,WEBER J T,et al. An outbreak of diarrhea and hemolytic uremic syndrome from Escherichia coli O157:H7 in fresh-pressed apple cider[J].Jama,1993,269(17):2217-2220.
[13] CODY S H,GLYNN M K,FARRAR J A,et al. An outbreak of Escherichia coli O157:H7 infection from unpasteurized commercial apple juice[J].Annals of Internal Medicine,1999,130(3):202-209.
[14] SWERDLOW D L,GRIFFIN P M. Duration of faecal shedding of Escherichia coli O157:H7 among children in day-care centres[J].The Lancet,1997,349(9054):745-746.
[15] MICHINO H,ARAKI K,MINAMI S,et al. Massive outbreak of Escherichia coli O157:H7 infection in schoolchildren in Sakai city,Japan, associated with consumption of white radish sprouts[J]. American Journal of Epidemiology,1999,150(8):787.
[16] ACKMAN A,MARKS B,MACK M,et al. Swimming-associated haemorrhagic colitis due to Escherichia coli O157:H7 infection:Evidence of prolonged contamination of a fresh water lake[J]. Epidemiology & Infection,1997,119(1):1-8.
[17] GRIFFIN P M,TAUXE R V. The epidemiology of infections caused by Escherichia coli O157:H7,other enterohemorrhagic Escherichia coli,and the associated hemolytic uremic syndrome[J]. Epidemiologic Reviews,1991,13(1):60.
[18] 古丽娜·巴克,余雄,黄锡霞,等.新疆奶牛业发展现状调查[J].畜牧与兽医,2016,48(10):66-69. GULINA B,YU X,HUANG X X,et al. Investigation on current situation of dairy production in Xinjiang area[J].Animal Husbandry & Veterinary Medicine,2016,48(10):66-69.(in Chinese)
[19] 张桃.牛源致病性大肠杆菌O157:H7的分离鉴定及对其小鼠疾病模型的中药治疗效果[D].乌鲁木齐:新疆农业大学,2016. ZHANG T.Isolation identification of E.coli O157:H7 strain from cattle and Chinese medicine treatment effect of the disease model of mouse[D]. Urumqi:Xinjiang Agricultural University,2016. (in Chinese)
[20] 丁浩,苏战强,夏利宁,等.免疫磁珠富集法对牛源大肠杆菌O157:H7分离鉴定的影响[J].中国畜牧兽医,2017,44(4):1189-1194. DING H,SU Z Q,XIA L N,et al.Influence of immunomagnetic enrichment method for isolation and identification of E.coli O157:H7 from bovine[J].China Animal Husbandry & Veterinary Medicine,2017,44(4):1189-1194. (in Chinese)
[21] VAN D J,GRAHAM T,GANNON V.The prevalence of verotoxins,Escherichia coli O157:H7 and Salmonella in the feces and rumen of cattle at processing[J].Canadian Veterinary Journal-Revue Veterinaire Canadienne,1999,40(5):332-338.
[22] CHAPMAN P A,SIDDONS C A,GERDAN MALO A T, et al. A 1-year study of Escherichia coli O157 in cattle, sheep pigs and poultry[J]. Epidemiology & Infection,1997,119(2):245-250.
[23] ELDER R O,KEEN J E,SIRAGUSA G R,et al. Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces,hides,and carcasses of beef cattle during processing[J]. Proceedings of the National Academy of Sciences of the United States of America,2000,97(7):2999-3003.
[24] VAN D J,BERG J,POTTER A,et al. Environmental sources and transmission of Escherichia coli O157 in feedlot cattle[J].Canadian Veterinary Journal,2001,42(9):714.
[25] SHERE J A,BARTLETT K J,KASPAR C W. Longitudinal study of Escherichia coli O157:H7 dissemination on four dairy farms in Wisconsin[J]. Applied & Environmental Microbiology,1998,64(4):1390.
[26] KING T,KOCHARUNCHITT C,GOBIUS K,et al. Physiological response of Escherichia coli O157:H7 Sakai to dynamic changes in temperature and water activity as experienced during carcass chilling[J]. Molecular & Cellular Proteomics:Mcp,2016,15(11):63065.
[27] 扈光辉,扈光强,郑鹏,等.不同因素对初产奶牛繁殖力的影响[J].江苏农业科学,2017,45(4):128-130. HU G H,HU G Q,ZHENG P,et al.Effects of different factors on fecundity of first-generation[J].Jiangsu Agricultural Sciences,2017,45(4):128-130.(in Chinese)

Study on Seasonal Impact to the Distribution of Bovine E.coli O157: H7 in Xinjiang

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	SONG Chenglei, ZHANG Zhe, WEI Yilin, YANG Songbai, DOU Yaqing, LI Chenlei, LIU Yingke, LI Xinjian, YANG Feng, LI Xiuling, WANG Kejun, QIAO Ruimin, HAN Xuelei. Effects of Different Breeds,Seasons and Month Ages on Semen Quality of Boars [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1938-1946.
[3]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[4]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[5]	HE Lin, HAN Diangang, LI Jing, DU Xuan, ZHANG Jiaxiang, XIN Jige. The Progress of the Detection Methods of Bovine Viral Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1593-1602.
[6]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[7]	ZHEN Zhen, WANG Mei, WANG Yimin, HU Debao, ZHANG Linlin, LI Xin, GUO Yiwen, GUO Hong, DING Xiangbin. Effect of RNA Methylation Transfer Enzyme METTL3 on the Proliferation and Myogenic Differentiation of Bovine Skeletal Muscle Satellite Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1025-1036.
[8]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.
[9]	XIANG Decai, LI Shuiying, ZHANG Bin, ZHANG Yan, LIANG Jiachong, LYU Chunrong, HONG Qionghua, QUAN Guobo, WU Guoquan. The Study on the Role of Fetal Bovine Serum Supplementation During Postwarming Culture on Vitrified Porcine Parthenogenetic Blastocysts [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 606-615.
[10]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[11]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[12]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[13]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[14]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.
[15]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.