猪德尔塔冠状病毒TJ1株的分离鉴定及生物学特性分析

doi:10.16431/j.cnki.1671-7236.2018.01.028

Abstract

Abstract:

The aim of this study was to obtain porcine deltacoronavirus (PDCoV) strain in China and study its biological characteristics. Porcine kidney (PK-15) cell monolayers were inoculated with the samples that were positive for PDCoV only by RT-PCR. PDCoV from the first passage was further serially passed in PK-15 cells. These cell cultures infected with PDCoV were confirmed by cytopathic effect (CPE), sequencing and analysis of PDCoV M gene, acid test; TCID₅₀ was confirmed by Reed-Muench method; The 11-day-old piglets were inoculated with 5×10^4.0 TCID₅₀/mL virus suspension and observed for clinical symptoms. The results indicated that the PDCoV was successfully isolated in cell culture and designated TJ₁, and the infectious titer of the 5th passage of TJ₁ strain was 10^4.5 TCID₅₀/mL; The isolated virus was resistant to chloroform and ether, it was stable at pH 3.0, and the virus was inactivated at 50℃ for 1 h; The TJ₁ M gene shared a homology of 94% to 99% with PDCoV M gene sequences published previously in GenBank; The animal pathogenicity test showed that diarrhea occurred in all piglets by TJ₁. This study successfully isolated a PDCoV TJ₁ strain, and these biological characteristics of the strain were studied preliminarily, which provided a foundation for further research on PDCoV.

Key words: porcine deltacoronavirus (PDCoV); isolation; identification; biological characteristics

CLC Number:

S858.28

ZHENG Li, LI Xiuli, YAN Minghua, REN Weike, ZHANG Lei, LU Chao, TIAN Xiangxue, HAN Wei. Isolation, Identification and Biological Characteristics Analysis of Porcine Deltacoronavirus TJ₁[J]. , 2018, 45(1): 219-224.

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Isolation, Identification and Biological Characteristics Analysis of Porcine Deltacoronavirus TJ₁

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Isolation, Identification and Biological Characteristics Analysis of Porcine Deltacoronavirus TJ1

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Isolation, Identification and Biological Characteristics Analysis of Porcine Deltacoronavirus TJ₁