水貂TYR基因T138A位点多态性及其与毛色性状的关联分析

doi:10.16431/j.cnki.1671-7236.2017.10.020

Abstract

Abstract:

In order to detect the polymorphism of T138A locus in tyrosinase (TYR) gene in mink and analyze the relationship between the genetic polymorphisms and phenotypes of mink hair color, the blood samples of 430 minks with five kinds of different hair color were taken and their genomic DNA were extracted. The T138A locus of TYR gene in mink was detected using PCR-RFLP method. Allele frequency and genotype frequency were calculated. Furthermore, the relationship between the polymorphism of T138A locus and hair color trait were analyzed by the statistical method of Chi-square independence test. The results showed that the T138A locus polymorphism was found with two alleles T and A,and three genotypes of TT, TA and AA. AA genotype was dominant genotype in Jilin White mink (0.9069), TT genotype was dominant genotype in Jinzhou Black mink,Pearl mink,Coffee mink and Silverblue mink, and existed mainly in Jizhou Black mink (1.0000). The association analysis of T138A locus polymorphism with hair color trait indicated that there was extremely significant correlation between TYR gene polymorphism and hair color of mink (P < 0.0001). This results indicated that the T138A locus might affect hair color phenotype, or molecular marker linked with the major gene regulating the white hair phenotype of mink.

Key words: mink; TYR gene; T138A locus; PCR-RFLP; hair color

CLC Number:

S813.3

SONG Xing-chao, XU Chao, LIU Zong-yue, YANG Tong-ao, YUE Zhi-gang, LIU Lin-ling, CONG Bo, YANG Fu-he. Relationship Analysis Between the Polymorphism of T138A Locus in TYR Gene and Hair Color in Mink (Neovison vison)[J]. , 2017, 44(10): 2973-2979.

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Relationship Analysis Between the Polymorphism of T138A Locus in TYR Gene and Hair Color in Mink (Neovison vison)

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