水貂ASIP基因单倍型检测及其皮肤组织mRNA差异表达分析

doi:10.16431/j.cnki.1671-7236.2019.10.021

Abstract

Abstract: The aim of this study was to explore the effects of haplotype formed by single nucleotide polymorphisms (SNPs) of agouti signaling protein (ASIP) gene and analyze the association of mRNA differential expression with coat color phenotype in American mink (Neovison vison).The SNPs haplotypes of ASIP gene in Jinzhou Black mink,Red eye White mink and Mingwei Silverblue mink were detected by PCR and Sanger direct sequencing technology.The mRNA expression levels of ASIP gene in skin tissue with three kinds of coat color were detected by Real-time PCR,and then the correlation between haplotypes,mRNA differential expression levels and coat color traits were analyzed.The results showed that a total of 10 SNPs were detected in 301 samples,of which 4 SNPs (G18A,A159G,G235T and C1189T) were located in intron 2,6 SNPs(C252T,A290C,G298C,A340G,T343C and T379C) were located in partial intron 3.10(Hap1-Hap10) and 4 (Hap1-Hap4) haplotypes were formed by 4 and 6 SNPs,respectively,of which Hap1 (GAGC) and Hap2 (GAGT) were shared haplotypes in intron 2,Hap2 (CCCGCC) was the main haplotype in Mingwei Silverblue mink.5 loci (A290C,G298C,A340G,T343C and T379C) were in complete linkage disequilibrium.Real-time PCR results showed that the mRNA expression levels of ASIP gene of Jinzhou Black mink and Mingwei Silverblue mink were 1.25 and 0.95 times,which were higher than that of Red eye White mink,there was no significant difference among three coat colors (P>0.05).The results indicated that the molecular mechanism of ASIP gene regulating the formation of mink coat color phenotype might be different.

Key words: mink; ASIP gene; haplotype; differential expression; coat color

CLC Number:

SONG Xingchao, LIU Linling, WANG Shuming, SONG Shanshan, XU Chao, YANG Tongao, LIU Zongyue, CONG Bo, ZHANG Zhiming, YANG Fuhe, PENG Yinghua. Haplotype Detection and mRNA Expression Level Analysis of ASIP Gene in Skin of American Mink (Neovison vison)[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 2998-3006.

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Haplotype Detection and mRNA Expression Level Analysis of ASIP Gene in Skin of American Mink (Neovison vison)

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