表达铜绿假单胞菌保护性抗原基因F1I2重组鼠伤寒沙门菌在水貂上的免疫原性研究

doi:10.16431/j.cnki.1671-7236.2020.04.021

Abstract

Abstract: The purpose of this study was to investigate the immunogenicity of recombinant Salmonella Typhimurium strain Δasdlh430(pYA-F1I2) carrying Pseudomonas aeruginosa protective antigen genes F_190-342(F1) and I_21-83(I2) in mink.Nine 7-month-old healthy minks were randomly divided into three groups,three in each group:Control group,hypodermic injection of sterile normal saline;LH430 strain immunization group,hypodermic injection of 2.0×10⁸ CFU per mink;Δasdlh430(pYA-F1I2) strain immunization group,hypodermic injection of 2.0×10⁸ CFU per mink.Every group was immunized once on the 15th day after the first immunization.On the 15th,30th and 45th day after the first immunization,the blood of mink was collected and the level of IgG in serum was measured.Peripheral blood monocytes were isolated on the 45th day after the first immunization.The number of F1I2 specific antibody secreting cells and Salmonella specific antibody secreting cells were detected by Eli-Spot.At the same time,lymphocytes were isolated from the spleen,and the specific lymphocyte proliferation of F1I2 and Salmonella was detected by MTT method.The results showed that the titers of F1I2 specific IgG antibody and Salmonella specific IgG antibody in serum of Δasdlh430(pYA-F1I2) group increased gradually at the sampling point,and reached the maximum value at the 45th day.On the 45th day after immunization,the number of F1I2 specific IgG secretory cells in Δasdlh430(pYA-F1I2) group was extremely significantly higher than that in control and LH430 groups (P < 0.01),and the number of Salmonella specific antibody secretory cells was extremely significantly higher than that of control group (P < 0.01),but there was no significant difference with LH430 group (P > 0.05).At the same time,F1I2 antigen and Salmonella antigen stimulated the proliferation of mink spleen lymphocytes significantly (P < 0.01).This study could provide a theoretical basis for the development of a new bivalent genetic engineering vaccine against mink hemorrhagic pneumonia and Salmonella infection.

Key words: Pseudomonas aeruginosa; mink; hemorrhagic pneumonia; immunogenicity

CLC Number:

S852.61

ZHANG Mingliang, SUN Changjiang, GU Jingmin, CUI Ziyin, HAN Wenyu. Immunogenicity of Recombinant Salmonella Typhimurium Expressing Protective Antigen Gene F1I2 of Pseudomonas aeruginosa in Mink[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1156-1162.

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Immunogenicity of Recombinant Salmonella Typhimurium Expressing Protective Antigen Gene F1I2 of Pseudomonas aeruginosa in Mink

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