禽源肠炎沙门菌aroA基因缺失株的构建及其生物学特性研究

doi:10.16431/j.cnki.1671-7236.2021.06.039

Abstract

Abstract: The puopose of the study was to analyze the effect of aroA gene on the biological characteristics and virulence of Salmonella Enteritidis.The aroA gene of Salmonella Enteritis isolated from poultry in the field was knocked out using λ-Red homologous recombination.The genetic stability of the gene deletion strain was tested by continuous passages.The differences between the wild strain and the gene deletion strain were compared by bacterial growth curve,the utilization of different biochemical reaction tubes,the ability of biofilm formation,the resistance to environmental stress,virulence to 1-day-old chicks.The results showed that the gene deletion strain was successfully constructed,and no revert mutation was found in the target gene after 30 successive generations.The deletion strains entered the logarithmic growth phase and stable phase at the same time compared with the wild strains under the same culture conditions,but the growth rate of the deletion strains was lower than that of the parent strains.The biochemical characteristics of the deletion strains were not changed compared with the wild strains.The ability of biofilm formation and resistance to acid,alkali,high osmolar and heat stress of the deletion strain were significantly lower than those of the wild strain (P<0.05).1-day-old chicks were inoculated through the mouth with the deletion strain and the wild strain respectively.All the chickens in the wild strain group were died after 14 days of observation,while none in the deletion strain group.In conclusion,the aroA gene deletion strain of Salmonella Enteritis with good genetic stability was successfully constructed and obtained in this experiment.The biochemical characteristics and in vitro growth trend of the deletion strain were not significantly changed,but the biofilm formation ability,resistance to environmental stress and virulence were significantly decreased.This study laid a foundation for the further study of the characteristics and immunogenicity of aroA gene in Salmonella Enteritis.

Key words: Salmonella Enteritis; aroA gene; immunogenicity

CLC Number:

S852.61

CUI Yi, WANG Zhenhua, ZHAO Sujuan, XU Jinpeng, YANG Kai, MA Xingshu. Construction and Biological Characteristics of aro A Gene Deletion Strain of Salmonella Enteritis from Poultry[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2247-2254.

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Construction and Biological Characteristics of aro A Gene Deletion Strain of Salmonella Enteritis from Poultry

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