甘氨酸对水貂GV期卵母细胞冷冻保存效率的影响

doi:10.16431/j.cnki.1671-7236.2019.07.014

Abstract

Abstract:

The purpose of this study was to investigate the effects of glycine (Gly) supplement on the survival rate,nuclear development,mitochondrial distribution and cortical granules distribution in mink oocytes during vitrification and in vitro maturation at germinal vesicle (GV) stage.The experiment was divided into three groups:Control group without vitrification,vitrification group and Gly supplement (1 mmol/L) vitrification group.Mink GV oocytes were cultured for 3 h after vitrification and in vitro maturation,respectively.Immunofluorescence labeling was used to detect the differences of mitochondrial distribution in GV oocytes and the changes of cortical granules distribution in MⅡ oocytes.The results showed that there was no significant difference in the survival rate of oocyte in Gly supplement vitrification group at 3 h after vitrification compared with vitrification group (P>0.05),but it was significantly lower than control group (P<0.05).The meiotic recovery rate of oocytes in Gly supplement vitrification group was significantly higher than vitrification group (P<0.05),but there was no significant difference with control group(P>0.05).The results of immunofluorescence showed that the normal distribution rate of mitochondria in GV oocytes of Gly supplement vitrification group was significantly higher than vitrification group (P<0.05),but the normal mitochondria distribution rate in GV oocytes of Gly supplement vitrification and vitrification groups were significantly lower than control group (P<0.05).The results of cortical granules distribution showed that when mink GV oocytes matured in vitro after vitrification to MⅡ stage,the proportion of cortical granules in Gly supplement vitrification group was significantly higher than vitrification group (P<0.05),but the proportion of cortical granules in Gly supplement vitrification and vitrification grous were significantly lower than control group (P<0.05).The results showed that the Gly supplement in mink oocytes could increase the meiotic recovery rate,and reduce the loss of mitochondria and cortical granules after vitrification.

Key words: glycine; mink; oocyte; vitrification

CLC Number:

S865.2

CAO Junguo, LI Wen, HAN Yuping, LI Xiaoxia, WANG Shiyong, DIAO Yunfei, YANG Yifeng, MENG Qingjiang, CAO Manyuan, CHANG Tong, WEI Haijun, XU Baozeng. Effects of Glycine on the Efficiency of Cryopreservation of Mink Oocytes at Germinal Vesicle Stage[J]. , 2019, 46(7): 1994-2002.

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Effects of Glycine on the Efficiency of Cryopreservation of Mink Oocytes at Germinal Vesicle Stage

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