利用微卫星标记鉴定德州驴亲子关系

doi:10.16431/j.cnki.1671-7236.2019.07.015

Abstract

Abstract:

This study was aimed to establish the parentage testing system of Dezhou donkey.13 microsatellite loci were selected as markers.A total of 53 blood samples of Dezhou donkey were collected,including 16 offsping,13 candidate fathers and 24 candidate mothers samples.The genomic DNA was obtained with phenol and chloroform method.The microsatellite loci were amplified by PCR and conducted gene scanning,the genotyping result was read using Peak Scanner Software v1.0.The genetic diversity of microsatellite loci were analyzed,the parentage relationship between individuals were identified using the likelihood-based (Cervus 3.0 software)and exclusion-based methods.The results showed that the average of alleles number,observed heterozygosity (Ho),expected heterozygosity (He) and polymorphism information content (PIC) were 6.846,0.689,0.671 and 0.625.The difference between expected heterozygosity and observed heterozygosity varied from 0.002 to 0.088.Their combined exclusion probability reached 0.990.The microsatellite loci showed high polymorphic and high exclusion probabilities,which made them suitable for genetic analysis and parentage testing.Most similar parents of 16 offspring donkeys were identified using Cervus 3.0 software.The genotypes of the 16 donkeys and their most likely parents were compared to exclude unrelated individuals.The parents of 11 donkeys were identified.In this experiment,13 microsatellite loci were used as the core markers,and the likelihood-based and exclusion-based methods were used as the main analytical method for the parentage testing system of Dezhou donkey,which provided the useful information for breeding work.

Key words: Dezhou donkey; microsatellite; parentage testing

CLC Number:

S813.3

WANG Min, ZHANG Xinhao, CUI Ran, LIU Yu, YANG Li, LI Haijing, ZHAO Chunjiang. Parentage Testing of Dezhou Donkey with Microsatellite Marker[J]. , 2019, 46(7): 2003-2011.

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Parentage Testing of Dezhou Donkey with Microsatellite Marker

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