3个品种兔微卫星标记的遗传多样性研究

doi:10.16431/j.cnki.1671-7236.2018.12.022

Abstract

Abstract:

This study was aimed to analyze the genetic variance in each breed and the genetic correlation among three rabbit breeds including Fujian Yellow rabbit,New Zealand rabbit and Japanese White rabbit.In this experiment,all blood samples were took from 25 rabbits of each breed,DNA from the blood was extracted by DNA extract kit.The target sequence was got using 15 microsatellite loci,fluorescent PCR and capillary electrophoresis.The genetic diversity of three rabbit breeds was discussed by calculating alleles,effective alleles,heterozygosity,polymorphic information content and genetic distance.The results showed that a total of 110 alleles were detected at 15 microsatellite loci in three rabbit breeds,and the average number of allele was 7.3.The number of alleles of Fujian Yellow rabbit,New Zealand rabbit and Japanese White rabbit were 95,95 and 88,respectively.The number of microsatellite loci with high polymorphism variability in three rabbit breeds were below:Fujian Yellow rabbit was 11,New Zealand rabbit was 12,Japanese White rabbit was 11,so the selected 15 microsatellite loci had rich polymorphic information in three rabbit breeds.The result of Hardy-Weinberg equilibrium test showed that 8 microsatellite loci deviated from the Hardy-Weinberg equilibrium in Fujian Yellow rabbit and New Zealand rabbit,and 6 microsatellite loci in Japanese White rabbit.Nei's genetic distance analysis showed that the genetic distances between Fujian Yellow rabbit and New Zealand rabbit,Fujian Yellow rabbit and Japanese White rabbit,New Zealand rabbit and Japanese White rabbit were 0.1761,0.2347 and 0.0432,respectively.The smaller genetic distance lead to the higher similarity and the closer genetic relationship.In a word,the genetic relationship between New Zealand rabbit and Japanese White rabbit was the most close in three rabbit breeds,and the genetic relationship between Fujian Yellow rabbit and Japanese White rabbit was the most distant in three rabbit breeds.

Key words: rabbit; microsatellite; genetic diversity

CLC Number:

S829.1

YU Chunying, YANG Yanyan, ZHOU Jianhua, LIN Wei, WANG Xunli. Study on Genetic Diversity of Microsatellite Markers in Three Rabbit Breeds[J]. , 2018, 45(12): 3486-3496.

References

[1] 卞吉.福建黄兔的品种特性及饲养管理[J].特种经济动植物,2015,2:3-4. BIAN J.Varietal characteristic and management of feeding in Fujian Yellow rabbit[J].Special Economic Animal and Plant,2015,2:3-4.(in Chinese)
[2] 郑秦文,陈思恋,林欣,等.蛋氨酸羟基类似物异丙酯(HMBi)对福建黄兔屠宰性能、肠道黏膜形态和肠道酶活的影响[J].中国畜牧兽医,2015,42(7):1711-1716. ZHENG Q W,CHEN S L,LIN X,et al.Effect of HMBi on slaughter performance,intestinal mucosal morphology and intestinal enzyme activity of Fujian Yellow rabbits[J].China Animal Husbandry &Veterinary Medicine,2015,42(7):1711-1716.(in Chinese)
[3] 陈岩锋,孙世坤,桑雷,等.黑白花兔与福建黄兔生长发育与及肉质特性的比较研究[J].畜牧与兽医,2018,50(1):15-18. CHEN Y F,SUN S K,SANG L,et al.Comparison of growth and meat quality between the Black White rabbit and Fujian Yellow rabbit[J].Animal Husbandry and Veterinary Medicine,2018,50(1):15-18.(in Chinese)
[4] 房晓欢,尹彦开,刘洋洋,等.深县猪遗传多样性监测适宜抽样规模的确定[J].中国畜牧兽医,2017,44(1):3250-3256. FANG X H,YIN Y K,LIU Y Y,et al.Determination of suitable sampling size in genetic diversity monitoring of Shenxian pig's population[J].China Animal Husbandry & Veterinary Medicine,2017,44(1):3250-3256.(in Chinese)
[5] 王思珍,郭闯,郭猛,等.巴马小型猪和贵州小型猪封闭群的遗传结构分析[J].中国兽医学报,2017,37(7):1389-1393. WANG S Z,GUO C,GUO M,et al.Population structure analysis of Bama and Guizhou miniature pig outbred population[J].Chinese Journal of Veterinary Science,2017,37(7):1389-1393.(in Chinese)
[6] 张相伦,游伟,靳青,等.4个肉牛品种微卫星多态性分析[J].中国畜牧兽医,2018,45(1):162-170. ZHANG X L,YOU W,JIN Q,et al.Analysis of microsatellite polymorphism among four beef cattle breeds[J].China Animal Husbandry & Veterinary Medicine,2018,45(1):162-170.(in Chinese)
[7] 娄渊根,刘丑生,刘建学,等.9个绵羊品种微卫星DNA遗传多样性分析[J].中国畜牧兽医,2011,38(10):106-112. LOU Y G,LIU C S,LIU J X,et al.Genetic diversity of microsatellite DNA in nine sheep breeds[J].China Animal Husbandry & Veterinary Medicine,2011,38(10):106-112.(in Chinese)
[8] 柴文琼,成述儒,靳建华,等.5个绵羊群体微卫星多态性分析[J].扬州大学学报(农业与生命科学版),2012,33(1):38-43. CHAI W Q,CHENG S R,JIN J H,et al.Analysis of microsatellite polymorphism in five sheep popula-tions[J].Journal of Yangzhou University (Agricultural and Life Science Edition),2012,33(1):38-43.(in Chinese)
[9] 刘伟,詹凯,郭盼盼,等.基于微卫星标记的安徽省四个地方鸡种群体遗传多样性及其遗传结构分析[J].中国家禽,2017,39(5):8-12. LIU W,ZHAN K,GUO P P,et al.Analysis on genetic diversity and population structure among four kinds of Anhui local chicken breeds using microsatellite markers[J].China Poultry,2017,39(5):8-12.(in Chinese)
[10] 赵东伟,李春苗,黎寿丰,等.13个地方鸡品种6个微卫星座位遗传多样性分析[J].扬州大学学报(农业与生命科学版),2017,38(4):39-45. ZHAO D W,LI C M,LI S F,et al.Analysis of genetic diversity of thirteen indigenous chicken breeds using six microsatellite loci[J].Journal of Yangzhou University (Agricultural and Life Science Edition),2017,38(4):39-45.(in Chinese)
[11] 李银银,吴绍亮,王洪,等.微卫星DNA分析国内24个近交系小鼠遗传状况[J].中国比较医学杂志,2017,27(8):43-49. LI Y Y,WU S L,WANG H,et al.Genetic quality analysis of 24 domestic inbred mouse strains by microsatellite DNA[J].Chinese Journal of Comparative Medicine,2017,27(8):43-49.(in Chinese)
[12] 洪杨,袁明铭,周静,等.应用微卫星标记对两个SD大鼠封闭群的遗传分析[J].实验动物与比较医学,2018,38(1):10-15. HONG Y,YUAN M M,ZHOU J,et al.Genetic analysis on two closed colonies of SD rats using microsatellite DNA markers[J].Laboratory Animal and Comparative Medicine,2018,38(1):10-15.(in Chinese)
[13] 陈民利,朱亮,蔡月琴,等.微卫星技术在大耳白黑眼兔近交系培育中的监测分析[J].动物学研究,2010,31(4):401-407. CHEN M L,ZHU L,CAI Y Q,et al.Monitoring inbreeding of WHBE rabbits using microsatellite[J].Zoological Research,2010,31(4):401-407.(in Chinese)
[14] 陈川,赵进,陈泓龙,等.微卫星标记在兔遗传育种中的应用[J].安徽农业科学,2011,39(1):279-281. CHEN C,ZHAO J,CHEN H L,et al.Application ofmicrosatellite makers on genetic breeding in rabbits[J].Journal of Anhui Agricultural Science,2011,39(1):279-281.(in Chinese)
[15] 杨国忠,刘芳,杨翠军,等.塞北兔群体遗传变异的微卫星标记[J].中国兽医学报,2016,36(1):172-174. YANG G Z,LIU F,YANG C J,et al.Genetic variability in Saibei rabbits with microsatellite markers[J].Chinese Journal of Veterinary Science,2016,36(1):172-174.(in Chinese)
[16] 黄江南,刘林秀,季华员,等.万载黑兔微卫星标记的遗传多样性分析[J].浙江农业学报,2016,28(4):574-579. HUANG J N,LIU L X,JI H Y,et al.Analysis on genetic diversity of Wanzai Black rabbit based on microsatellite markers[J].Acta Agriculturae Zhejiangensis,2016,28(4):574-579.(in Chinese)
[17] 刘庆华,黄柳英,梁学武.福建黄兔的遗传多态性及分类[J].中国农学通报,2008,24(8):6-10. LIU Q H,HUANG L Y,LIANG X W.Genetic diversity and classification of Fujian Yellow rabbit[J].Chinese Agricultural Science Bulletin,2008,24(8):6-10.(in Chinese)
[18] 谢喜平,陈岩锋,孙世坤,等.福建黄兔微卫星标记的遗传多样性分析[J].福建农业学报,2008,23(3):239-243. XIE X P,CHEN Y F,SUN S K,et al.Determination of genetic characteristics of Fujian Yellow rabbit population using microsatellite markers[J].Fujian Journal of Agricultural Sciences,2008,23(3):239-243.(in Chinese)
[19] KORSTANJE R,GILLISSEN G F,KODDE L P,et al.Mapping of microsatellite loci and association of aorta atherosclerosis with LG VI markers in the rabbit[J].Physiological Genomics,2001,6(1):11-18.
[20] KORSTANJE R,GILLISSEN G F,VERSTEEG S A,et al.Mapping of rabbit microsatellite markers using chromosome-specific libraries[J].Journal of Heredity,2003,94(2):161-169.
[21] RICO C,RICO I,WEBB N,et al.Four polymorphic microsatellite loci for the European wild rabbit,Oryctolagus cuniculus[J].Animal Genetics,1994,25(5):367.
[22] MOUGEL F,MOUNOLOU J C,MONNEROT M.Nine polymorphic microsatellite loci in the rabbit,Oryctolagus cuniculus[J].Animal Genetics,1997,28(1):58-71.
[23] CELINE C D,CELINE U,HELENE H,et al.Construction of a cytogenetically anchored microsatellitemap in rabbit[J].Mammal Genome,2005,16(6):442-459.
[24] SURRIDGE A K,BELL D J,RICO C,et al.Polymorphic microsatellite loci in the European rabbit(Oryctolagus cuniculus)are also amplified in other lagomorph species[J].Animal Genetics,1997,28(4):302-305.
[25] 陈民利,蔡月琴,陶涛,等.微卫星标记对WHBE兔封闭群、日本大耳白兔和新西兰兔的遗传分析[J].中国比较医学杂志,2008,18(9):21-27. CHEN M L,CAI Y Q,TAO T,et al.Genetic analysis of WHBE rabbit,Japanese rabbit and New Zealand rabbit by microsatellite loci[J].Chinese Journal ofComparative Medicine,2008,18(9):21-27.(in Chinese)
[26] 樊兆斌,姜莉莉,杨福合.利用微卫星标记分析5个家兔品种的遗传变异[J].中国农学通报,2011,27(7):326-330. FAN Z B,JIANG L L,YANG F H.Studies on the genetic variability of five rabbits lines by microsatellite DNA[J].Chinese Agricultural Science Bulletin,2011,27(7):326-330.(in Chinese)
[27] 高子淇,刘先菊,荣蓉,等.实验兔三个封闭群微卫星DNA多态性遗传分析[J].中国实验动物学报,2012,20(2):26-31. GAO Z Q,LIU X J,RONG R,et al.Genetic analysis for three closed laboratory rabbit colonies using microsatellite loci[J].Acta Laboratorium Animals Scientia Sinica,2012,20(2):26-31.(in Chinese)
[28] BOTSTEIN D,WHITE R L,SKOLNICK M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].AmericanJournal of Human Genetics,1980,32(3):314-331.
[29] BARKE R.Estimation of average heterozygosity andgenetic distance from a small number of individuals[J].Genetics,1978,89(3):583-590.

Study on Genetic Diversity of Microsatellite Markers in Three Rabbit Breeds

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHONG Hualing, HE Congcong, WU Bangyuan. Effects of Fresh Alfalfa and Full-price Pellet Feed on the Antioxidant Properties and Muscle Structure of Rabbit Stomach [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 143-150.
[2]	ZHAO Fengmiao, GUO Ting, ZHOU Yaping, ZHAO Hongmei, WANG Yuchen, TIAN Guangyuan, SUN Yajie, BIAN Yuchen, YU Jialiang, HAO Yongqing. Pathogenicity Analysis of Bovine Viral Diarrhea Virus and Immune Effect Evaluation of E2 Protein in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3549-3558.
[3]	XIONG Chenyong, YIN Yanwen, SHI Kaichuang, LI Jun, ZHENG Min, WEI Xiankai, FENG Shuping, LONG Feng, QU Sujie, LU Wenjun, ZHOU Hongjin, HUANG Hailian, XIE Shouyu, LI Zongqiang. Genetic Diversity Analysis of Duck Tembusu Virus in Guangxi During 2019-2021 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3099-3111.
[4]	BAI Shaocheng, ZHOU Juan, JIN Rongshuai, WANG Fan, LU Tingting, TANG Xianwei, ZHAO Bohao, WU Xinsheng, CHEN Yang. Cloning and Bioinformatics Analysis of CYP11A1 Gene in New Zealand White Rabbits and Its Effects on Reproduction-related Genes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2484-2496.
[5]	PENG Caichun, LI Binqiang, WANG Yeying, SU Haijun. Genetic Diversity Analysis of Sus scrofa Population in Guizhou Based on Mitochondrial CytbGene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2601-2612.
[6]	TIE Yanan, YANG Xixi, WANG Zhiliang, WANG Xinrong. Morphological and Histological Characteristics of Testis and Epididymis of Hyla Rabbits at Different Ages Before Sexual Maturity [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2645-2653.
[7]	ZHANG Qiang, LUOSANG Dunzhu, BASANG Wangdui, PENG Yangyang, E Guangxin, NIMA Ciren, SUOLANG Duoji, BA Duo, DAN Ba, XIAN Lili, DANBA Jiacan, ZHI Zhang, PINGCUO Zhandui. Estimation of Genetic Diversity and Population Structure of Five Tibet Yak Populations Using Microsatellites Markers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2228-2238.
[8]	WANG Zhiliang, YANG Xixi, TIE Yanan, FU Xiaoyu, WANG Xinrong. Effects of Superovulation on Morphology and Hormone Levels of Reproductive Organs in Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2250-2258.
[9]	GUO Luanying, LI Jialei, JIN Qinqin, MA Jun, LIU Quan. Research Progress on Bovine Hepacivirus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1994-2000.
[10]	LIU Xiaowei, LI Ruixiang, WANG Xinglong, LI Xiao. Genetic Diversity Analysis of Shaanxi Moschus berezovskii Based on mtDNA Cytb Gene and D-loop Region Sequence [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1352-1363.
[11]	BO Xinyu, TAO Xiaozhao, REN Zhanjun, DONG Xianggui, WANG Shuhui. Research Progress on β-NGF in Induced Ovulation in Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4288-4295.
[12]	WANG Yuanlang, DING Haisheng, ZHAO Huiling, HUANG Dongwei. Research Progress on Genetic Breeding and Reproduction of Domestic and International Rabbits in 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3918-3924.
[13]	WANG Yuanlang, DING Haisheng, ZHAO Huiling, HUANG Dongwei. Research Progress on Genetic Breeding and Reproduction of Domestic and International Rabbits in 2020 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 248-255.
[14]	LI Zhongbo, HOU Qianghong. Sequencing and Sequence Analysis of Ribosomal 18S rRNA and Mitochondrial cox1 Gene of Aedes albopictus in Huaihua District,Hunan Province [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2736-2745.
[15]	FANG Ruixin, TIAN Xueqi, ZOU Chen, DONG Yimeng, LI Yang, XING Xiumei, LIU Xin. Analysis of Genetic Diversity and Paternal Type of Red Deer Using SRY Gene in Y Chromosome [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2457-2466.