水貂PRLR基因的克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2019.02.003

Abstract

Abstract:

The aim of this study was to clone the full-length coding sequence of prolactin receptor (PRLR) gene from mink ovary tissues and analyze its biological characteristics to lay a foundation for studying its structure and function.A pair of primers was designed based on the predicted mRNA sequence of the dog PRLR gene in GenBank (accession No.:XM_025436332.1),and the ovary tissue samples of sexually mature healthy mink were collected,and RNA was extracted and then was reverse transcribed to synthesize cDNA,and PRLR gene was amplified by PCR using cDNA as template.The PCR products then were inserted into the pEASY-Blunt Simple Vector.Bioinformatics analysis was carried out after positive clone sequencing.The results showed that the coding region of PRLR gene of mink was 1 878 bp in length and encoded 625 amino acids.The homology of nucleotide sequence of PRLR gene between mink and Enhydra lutris kenyoni was the highest which reached 97.7%.The phylogenetic tree analysis also showed the closest relationship with Enhydra lutris kenyoni.The bioinformatics analysis of the PRLR protein of mink ovary showed that it contained a signal peptide,an extracellular region,a transmembrane region and an intramembranous region.It was a single transmembrane protein,and the D1 domain at the N-terminal contained two pairs of conserved disulfide bonds (Cys36-Cys46 and Cys75-Cys86),the D2 domain contained a conserved WS-motif (WS-E-WS).The PRLR protein of mink had a similar structure to other PRLR proteins.In conclusion,the study provided basic experimental data and theoretical basis for the further study of the structure and function of PRLR protein.

Key words: mink; PRLR gene; cloning; bioinformatics analysis

CLC Number:

WANG Liying, ZHANG Yufei, CAO Manyuan, ZHAO Weigang, XU Baozeng. Cloning and Bioinformatics Analysis of Prolactin Receptor Gene of Mink[J]. , 2019, 46(2): 345-353.

References

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Cloning and Bioinformatics Analysis of Prolactin Receptor Gene of Mink

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