microRNA-425-5p对3T3-L1前体脂肪细胞增殖、分化的影响

doi:10.16431/j.cnki.1671-7236.2019.02.004

Abstract

Abstract:

In order to explore the effect of miR-425-5p on the proliferation and differentiation of 3T3-L1 preadipocyte,the expression of miR-425-5p in tissues and cells were measured by Real-time PCR,the effect of miR-425-5p on the proliferation and differentiation of preadipocyte were measured by CCK8,EdU,oil red O staining and triglyceride detection methods,respectively.The target genes of miR-425-5p regulation preadipocyte differentiation were predicted and identified using bioinformatics softwares and dual luciferase reporter assays,respectively.The results showed that miR-425-5p was expressed at lower levels in adipose tissues of obese mice,and dynamically expressed during preadipocyte proliferation and differentiation.Compared with negative control,miR-425-5p overexpression promoted preadipocyte proliferation,inhibited marker genes related to adipocyte differentiation such as PPARγ,C/EBPα and FAS genes,and reduced lipid droplets and triglyceride accumulation.Inhibition of miR-425-5p expression inhibited the proliferation and prevented the differentiation of preadipocyte.Further analysis found that overexpression or inhibition of miR-425-5p decreased or increased IGF1 gene expression in preadipocyte differentiation,respectively.Compared with negative control,miR-425-5p overexpression inhibited the fluorescence activity of IGF1 gene 3'-UTR,whereas mutation of the miR-425-5p binding site in IGF1 gene 3'-UTR completely abolished this response.This results indicated that miR-425-5p might promote 3T3-L1 preadipocyte proliferation,whereas negatively regulates its differentiation by targeting IGF1.

Key words: miRNA-425-5p; preadipocyte; proliferation; differentation; IGF1 gene

CLC Number:

Q591.5

YANG Qiong, GU Hao, DU Jingjing, LIU Jinyuan, ZHANG Shunhua, ZHU Li. Effect of miR-425-5p on Proliferation and Differentiation of 3T3-L1 Preadipocyte[J]. , 2019, 46(2): 354-364.

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Effect of miR-425-5p on Proliferation and Differentiation of 3T3-L1 Preadipocyte

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