边鸡IGF-ⅠR基因AluⅠ位点多态性及其对生长性状和繁殖性状的遗传效应分析

doi:10.16431/j.cnki.1671-7236.2017.03.027

Abstract

Abstract:

Single nucleotide polymorphisms (SNPs) in exon 2 of insulin-like growth factor Ⅰ receptor (IGF-ⅠR) gene were detected by PCR-RFLP in Bian chicken,which was to find the correlation markers of growth and reproduction traits in Bian chicken.The results showed that AluⅠsite displayed polymorphic, and a G→A mutation at the position of the 376 bp in exon 2 was occurred. There were three genotypes (GG, GA and AA), the genotypes frequencies were 0.687, 0.296 and 0.017, respectively, GG genotype was the dominant genotype, the frequency of G allele was 0.835, which was the dominant allele. This site was in Hardy-Weinberg equilibrium (P>0.05).Correlation analysis showed that GG genotype had significantly higher body weight than those of GA genotype at the age of 8, 14, 16 and 18 weeks (P<0.05);The GG geno type had extremely significantly higher body weight at first egg than those of GA genotype (P<0.01),but it didn't mean that this locus could be used as a genetic marker for reproduction traits in Bian chicken. This results suggested that the AluⅠsite (G→A) in exon 2 of IGF-ⅠR gene could be used as a genetic marker for growth traits in Bian chicken.

Key words: Bian chicken; IGF-ⅠR gene; growth traits; reproduction traits; PCR-RFLP

CLC Number:

S813.3

WU Peng-fei, WANG Dong, ZHANG Xiang-qian, ZHANG Li, DIGN Fu-xiang, ZHANG Gen-xi. Study on the Polymorphisms of IGF-ⅠR Gene Alu Ⅰ Locus and Genetic Effect on Growth and Reproduction Traits in Bian Chicken[J]. , 2017, 44(3): 813-818.

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Study on the Polymorphisms of IGF-ⅠR Gene Alu Ⅰ Locus and Genetic Effect on Growth and Reproduction Traits in Bian Chicken

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