香猪FSHR基因外显子10的多态性及其与产仔数间的关联分析

doi:10.16431/j.cnki.1671-7236.2017.03.025

Abstract

Abstract:

Follicle-stimulating hormone (FSH), a glycoprotein secreted from pituitary, can regulate ovarian function through the FSH receptor (FSHR).To evaluate the effects of FSHR gene on the litter size of Xiang pig population, the polymorphisms of FSHR gene exon 10 were detected by polymerase chain reaction (PCR) and sequencing methods, and the effect of polymorphisms in FSHR gene exon 10 on litter size traits in Xiang pigs was analyzed using the least square analysis.In eight candidate sites, there were two polymorphic loci were detected out from Xiang pig population, which were rs322800083 (C276T) and rs332115220 (T601C). Moreover, a new SNP novel 1 (G-92A) was discovered.Four haplotypes were constructed between rs322800083 and rs332115220 sites, in which C-T was the main haplotype (45.0%) and presented closely linkage disequilibrium (r²=0.334) in Xiang pig population. Furthermore, the haplotype C-T corresponded to the highest total number born (TNB) at the second fetus of Xiang pigs, haplotype C-C to the lowest TNB. It was notified that haplotype C-T produced 0.99 TNB of Xiang pigs more than haplotype C-C (P<0.05). And the TNB at the third fetus was also the highest in haplotype C-T, which produced 1.61 and 2.33 TNB more than haplotypes T-C and C-C (P<0.01). The genotypes of locus rs332115220 was weakly correlated with the third fetus of Xiang pig population (ρ=0.429).The results suggested that haplotype C-T could be used as a molecular marker to assist selection for the litter size of Xiang pigs.

Key words: FSHR gene; polymorphism; Xiang pigs; molecular marker

CLC Number:

S813.3

XU Yao, LIU Chang, NIU Xi, RAN Xue-qin, WANG Jia-fu. Polymorphism of FSHR Gene Exon 10 and Its Relationship with the Litter Size of Xiang Pigs[J]. , 2017, 44(3): 799-806.

References

[1] Fan Q R, Hendrickson W A. Structure of human follicle-stimulating hormone in complex with its receptor[J]. Nature, 2005, 433(7023):269-277.
[2] Simoni M, Gromoll J, Nieschlag E. The follicle-stimulating hormone receptor:Biochemistry, molecular biology, physiology, and pathophysiology[J]. Endocrine Reviews, 1997, 18(6):739-773.
[3] Jiang X, Liu H, Chen X, et al. Structure of follicle-stimulating hormone in complex with the entire ectodomain of its receptor[J]. Proceedings of the National Academy of Sciences, 2012, 109(31):12491-12496.
[4] Daelemans C, Smits G, De M V, et al. Prediction of severity of symptoms in iatrogenic ovarian hyperstimulation syndrome by follicle-stimulating hormone receptor Ser680Asn polymorphism[J]. Journal of Clinical Endocrinology & Metabolism, 2004, 89(12):6310-6315.
[5] Gu B H, Park J M, Baek K H. Genetic variations of follicle stimulating hormone receptor are associated with polycystic ovary syndrome[J]. International Journal of Molecular Medicine, 2010, 26(1):107-112.
[6] 张爱萍, 张学红, 贾学玲,等. 卵泡刺激素受体基因多态性与西北地区汉族妇女卵巢反应性的关系[J]. 中国优生与遗传杂志, 2014,8:12-14.
[7] 李雯, 罗虹, 柴志欣,等. 牦牛FSHR基因5'端部分序列克隆及生物信息学分析[J]. 家畜生态学报, 2014, 35(1):15-20.
[8] 刘成武, 杨敏. 德国牧羊犬FSHR基因第1外显子SNP T89C与产仔数关系研究[J]. 黑龙江畜牧兽医, 2014,17:66-68.
[9] 郭宗义. 从江香猪产业可持续发展对策探讨[J]. 猪业科学, 2013,7:125-127.
[10] 杨廷模, 刘畅, 赵恒鑫,等. 高产从江香猪资源调查及其FSH基因型分析[J]. 畜牧与兽医, 2014, 46(8):5-8.
[11] 谢健, 喻昌燕, 许瑶,等. 可乐猪乳头数性状全基因组关联分析[J]. 中国畜牧兽医, 2016, 43(11):2866-2872.
[12] Zhang X D, Zhu H Y, Zhou J, et al. Relationship between polymorphisms in exon 10 of FSHR gene and litter size in swine[J]. Genetics & Molecular Research Gmr, 2015, 14(3):8252-8261.
[13] Demars J, Fabre S, Sarry J, et al. Genome-wide association studies identify two novel BMP15 mutations responsible for an atypical hyperprolificacy phenotype in sheep[J]. PLoS Genetics, 2013, 9(4):269-271.
[14] 李俊, 张勇, 杨红,等. 从江香猪BMP15基因多态性研究及单倍型构建[J]. 中国畜牧杂志, 2016, 52(7):5-11.
[15] Achrekar S K, Modi D N, Meherji P K, et al. Follicle stimulating hormone receptor gene variants in women with primary and secondary amenorrhea[J]. Journal of Assisted Reproduction and Genetics, 2010, 27(6):317-326.
[16] Grigorova M, Punab M, Poolamets O, et al. Study in 1790 Baltic men:FSHR, Asn680Ser polymorphism affects total testes volume[J]. Andrology, 2013, 1(2):293-300.
[17] 谭广梅, 李拥军, 张丽. 海门山羊FSHR基因外显子10的PCR-SSCP分析[J]. 中国草食动物科学, 2008, 28(4):12-14.
[18] Rahal P. Polymorphisms in the bovine follicle-stimulating hormone receptor gene[J]. Animal Genetics, 2000, 31(4):280-281.
[19] 于艳, 李建平,侯磊,等. 山羊FSHR基因第10外显子序列的克隆与比较研究[J]. 家畜生态学报, 2007, 28(1):70-74.
[20] 吴井生, 王金玉. FSHR基因第10外显子多态性及其与小梅山猪产仔数的相关性[J]. 中国农业科学, 2012, 45(13):2728-2736.
[21] 王玉金, 刘杏. 贵州黑山羊FSHR基因与繁殖性状相关性研究[J]. 中国畜禽种业, 2015,12:78-79.
[22] 雷雪芹, 陈宏, 袁志发,等. 牛FSHR基因第10外显子单核苷酸多态性及其与双胎性状的关系[J]. 中国生物化学与分子生物学报, 2004, 20(1):34-37.
[23] 余新沛, 吕成伟, 葛争鸣,等. 中国白族人群MBL基因SNP及其单倍型与基因型的研究[J]. 中国免疫学杂志, 2006, 22(8):738-742.
[24] Nejentsev S, Godfrey L, Snook H, et al. Comparative high-resolution analysis of linkage disequilibrium and tag single nucleotide polymorphisms between populations in the vitamin D receptor gene[J]. Human Molecular Genetics, 2004, 13(15):1633-1639.

Polymorphism of FSHR Gene Exon 10 and Its Relationship with the Litter Size of Xiang Pigs

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[2]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[3]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[4]	SUN Yanyong, MA Fengying, GUO Lili, LIU Zaixia, LIU Bin, ZHANG Wenguang, LIU Yongbin. Advances on the Application of Animal Blood Transcriptome [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 460-468.
[5]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[6]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[7]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[8]	HUANG Yueli, RAN Xueqin, NIU Xi, LI Sheng, HUANG Shihui, WANG Jiafu. Study on 264 bp Structural Variation of 3'-flanking Region of CHD3 Gene in Xiang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3026-3035.
[9]	DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.
[10]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[11]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[12]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[13]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[14]	ZHANG Yonghao, XIAO Yi, GUO Lijun, Tuerxun·Abudoureheman, WANG Yutao. Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 962-972.
[15]	FANG Qianhai, CHEN Hongbo, BI Yanzhen. Research Progress on Important Breeding Value Genes and Screening Techniques of Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 992-1004.